Immediate Insulin Treatment Prevents Diabetes-Induced Gut Region-Specific Increase in the Number of Myenteric Serotonergic Neurons
To evaluate the effects of hyperglycemia and insulin treatment on the proportion of serotonin-immunoreactive (5-HT-IR) myenteric neurons, samples were taken from the duodenum, ileum, and colon of diabetic, insulin-treated diabetic, and control rats 10 weeks after the onset of streptozotocin-induced...
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MDPI AG
2021-06-01
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| Online Access: | https://www.mdpi.com/2076-3417/11/13/5949 |
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| author | Diána Mezei Nikolett Bódi Zita Szalai Zsuzsanna Márton János Balázs Mária Bagyánszki |
| author_facet | Diána Mezei Nikolett Bódi Zita Szalai Zsuzsanna Márton János Balázs Mária Bagyánszki |
| author_sort | Diána Mezei |
| collection | DOAJ |
| description | To evaluate the effects of hyperglycemia and insulin treatment on the proportion of serotonin-immunoreactive (5-HT-IR) myenteric neurons, samples were taken from the duodenum, ileum, and colon of diabetic, insulin-treated diabetic, and control rats 10 weeks after the onset of streptozotocin-induced hyperglycemia. Myenteric whole-mount preparations were immunostained with anti-5-HT and pan-neuronal anti-HuCD markers. In controls, the 5-HT-IR myenteric neurons represent a small proportion (~2.5%) of the total neuronal number in the investigated gut segments. The proportion of 5-HT-IR myenteric neurons was significantly higher in the duodenum (<i>p</i> < 0.01) and colon (<i>p</i> < 0.0001) of diabetic rats compared to the controls but exhibited a slight increase in the ileum. Immediate insulin treatment resulted in a significantly lower proportion of myenteric 5-HT-IR neurons in each segment (duodenum <i>p</i> < 0.0001; ileum <i>p</i> < 0.01; and colon <i>p</i> < 0.0001) compared to the untreated diabetics. Our study demonstrates that the proportion of 5-HT-IR myenteric neurons was enhanced in type 1 diabetes in a region-specific manner. Immediate insulin treatment prevents a higher hyperglycemia-induced amount of 5-HT-IR neurons and restores it to the control level in each investigated gut segment. Despite the low proportion of 5-HT-IR myenteric neurons, hyperglycemia-related changes of these neurons may play a crucial role in gastrointestinal symptoms in type 1 diabetes. |
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| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T10:01:27Z |
| publishDate | 2021-06-01 |
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| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-382f2e7aab0b465bb6d566bef223686b2023-11-22T01:53:05ZengMDPI AGApplied Sciences2076-34172021-06-011113594910.3390/app11135949Immediate Insulin Treatment Prevents Diabetes-Induced Gut Region-Specific Increase in the Number of Myenteric Serotonergic NeuronsDiána Mezei0Nikolett Bódi1Zita Szalai2Zsuzsanna Márton3János Balázs4Mária Bagyánszki5Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, HungaryDepartment of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, HungaryDepartment of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, HungaryDepartment of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, HungaryDepartment of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, HungaryDepartment of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, HungaryTo evaluate the effects of hyperglycemia and insulin treatment on the proportion of serotonin-immunoreactive (5-HT-IR) myenteric neurons, samples were taken from the duodenum, ileum, and colon of diabetic, insulin-treated diabetic, and control rats 10 weeks after the onset of streptozotocin-induced hyperglycemia. Myenteric whole-mount preparations were immunostained with anti-5-HT and pan-neuronal anti-HuCD markers. In controls, the 5-HT-IR myenteric neurons represent a small proportion (~2.5%) of the total neuronal number in the investigated gut segments. The proportion of 5-HT-IR myenteric neurons was significantly higher in the duodenum (<i>p</i> < 0.01) and colon (<i>p</i> < 0.0001) of diabetic rats compared to the controls but exhibited a slight increase in the ileum. Immediate insulin treatment resulted in a significantly lower proportion of myenteric 5-HT-IR neurons in each segment (duodenum <i>p</i> < 0.0001; ileum <i>p</i> < 0.01; and colon <i>p</i> < 0.0001) compared to the untreated diabetics. Our study demonstrates that the proportion of 5-HT-IR myenteric neurons was enhanced in type 1 diabetes in a region-specific manner. Immediate insulin treatment prevents a higher hyperglycemia-induced amount of 5-HT-IR neurons and restores it to the control level in each investigated gut segment. Despite the low proportion of 5-HT-IR myenteric neurons, hyperglycemia-related changes of these neurons may play a crucial role in gastrointestinal symptoms in type 1 diabetes.https://www.mdpi.com/2076-3417/11/13/5949diabetic enteropathygut segment specificitymyenteric neuronsserotonintype 1 diabetesenteric nervous system |
| spellingShingle | Diána Mezei Nikolett Bódi Zita Szalai Zsuzsanna Márton János Balázs Mária Bagyánszki Immediate Insulin Treatment Prevents Diabetes-Induced Gut Region-Specific Increase in the Number of Myenteric Serotonergic Neurons Applied Sciences diabetic enteropathy gut segment specificity myenteric neurons serotonin type 1 diabetes enteric nervous system |
| title | Immediate Insulin Treatment Prevents Diabetes-Induced Gut Region-Specific Increase in the Number of Myenteric Serotonergic Neurons |
| title_full | Immediate Insulin Treatment Prevents Diabetes-Induced Gut Region-Specific Increase in the Number of Myenteric Serotonergic Neurons |
| title_fullStr | Immediate Insulin Treatment Prevents Diabetes-Induced Gut Region-Specific Increase in the Number of Myenteric Serotonergic Neurons |
| title_full_unstemmed | Immediate Insulin Treatment Prevents Diabetes-Induced Gut Region-Specific Increase in the Number of Myenteric Serotonergic Neurons |
| title_short | Immediate Insulin Treatment Prevents Diabetes-Induced Gut Region-Specific Increase in the Number of Myenteric Serotonergic Neurons |
| title_sort | immediate insulin treatment prevents diabetes induced gut region specific increase in the number of myenteric serotonergic neurons |
| topic | diabetic enteropathy gut segment specificity myenteric neurons serotonin type 1 diabetes enteric nervous system |
| url | https://www.mdpi.com/2076-3417/11/13/5949 |
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