Metagenomic survey of bacteria associated with the invasive ladybird Harmonia axyridis (Coleoptera: Coccinellidae)
The Asian ladybird Harmonia axyridis is an invasive insect in Europe and the Americas and is a great threat to the environment in invaded areas. The situation is exacerbated by the fact that non native species are resistant to many groups of parasites that attack native insects. However, very little...
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| Format: | Article |
| Language: | English |
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Institute of Entomology, Biology Centre, Czech Academy of Science
2017-06-01
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| Series: | European Journal of Entomology |
| Subjects: | |
| Online Access: | https://www.eje.cz/artkey/eje-201701-0038_Metagenomic_survey_of_bacteria_associated_with_the_invasive_ladybird_Harmonia_axyridis_Coleoptera_Coccinellid.php |
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| author | Krzysztof DUDEK Kinga HUMIŃSKA Jacek WOJCIECHOWICZ Piotr TRYJANOWSKI |
| author_facet | Krzysztof DUDEK Kinga HUMIŃSKA Jacek WOJCIECHOWICZ Piotr TRYJANOWSKI |
| author_sort | Krzysztof DUDEK |
| collection | DOAJ |
| description | The Asian ladybird Harmonia axyridis is an invasive insect in Europe and the Americas and is a great threat to the environment in invaded areas. The situation is exacerbated by the fact that non native species are resistant to many groups of parasites that attack native insects. However, very little is known about the complex microbial community associated with this insect. This study based on sequencing 16S rRNA genes in extracted metagenomic DNA is the first research on the bacterial flora associated with H. axyridis. Lady beetles were collected during hibernation from wind turbines in Poland. A mean ± SD of 114 ± 35 species of bacteria were identified. The dominant phyla of bacteria recorded associated with H. axyridis were Actinobacteria, Proteobacteria, Firmicutes and Bacteroidetes. Representatives of these phyla are common in the environment, e.g. in the soil, and are often identified as the dominant bacteria associated with arthropods. We also identified animal pathogenic bacteria, such as Burkholderia, Rhodococcus, Chlamydiae and Anaplasmataceae spp. (Neorickettsia helminthoeca and Ehrlichia ovina). We also identified Wolbachia pipientis in a single beetle. This bacterium is a causative agent of reproductive alterations in arthropods. These results support the enemy release hypothesis in the case of this ladybird invasion. Pathogenic bacteria were recorded in only a few samples. Moreover, male-killing bacteria such as Spiroplasma spp., Wolbachia spp. and Rickettsia spp. were only recorded in single insects so they cannot be responsible for the observed alterations in the sex-ratio of the ladybird population studied. |
| first_indexed | 2024-12-14T21:10:20Z |
| format | Article |
| id | doaj.art-382fd3c3ce30421781ea548936393405 |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-14T21:10:20Z |
| publishDate | 2017-06-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-382fd3c3ce30421781ea5489363934052022-12-21T22:47:17ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292017-06-01114131231610.14411/eje.2017.038eje-201701-0038Metagenomic survey of bacteria associated with the invasive ladybird Harmonia axyridis (Coleoptera: Coccinellidae)Krzysztof DUDEK0Kinga HUMIŃSKA1Jacek WOJCIECHOWICZ2Piotr TRYJANOWSKI3Department of Zoology, Institute of Zoology, Poznań University of Life Sciences, Wojska Polskiego 71 C, 60-625 Poznań, Poland; e-mails: dudeekk@gmail.com, piotr.tryjanowski@gmail.comDNA Research Center, Rubież 46, 61-612 Poznań, Poland; e-mails: k.huminska@cbdna.pl, j.wojciechowicz@cbdna.plDNA Research Center, Rubież 46, 61-612 Poznań, Poland; e-mails: k.huminska@cbdna.pl, j.wojciechowicz@cbdna.plDepartment of Zoology, Institute of Zoology, Poznań University of Life Sciences, Wojska Polskiego 71 C, 60-625 Poznań, Poland; e-mails: dudeekk@gmail.com, piotr.tryjanowski@gmail.comThe Asian ladybird Harmonia axyridis is an invasive insect in Europe and the Americas and is a great threat to the environment in invaded areas. The situation is exacerbated by the fact that non native species are resistant to many groups of parasites that attack native insects. However, very little is known about the complex microbial community associated with this insect. This study based on sequencing 16S rRNA genes in extracted metagenomic DNA is the first research on the bacterial flora associated with H. axyridis. Lady beetles were collected during hibernation from wind turbines in Poland. A mean ± SD of 114 ± 35 species of bacteria were identified. The dominant phyla of bacteria recorded associated with H. axyridis were Actinobacteria, Proteobacteria, Firmicutes and Bacteroidetes. Representatives of these phyla are common in the environment, e.g. in the soil, and are often identified as the dominant bacteria associated with arthropods. We also identified animal pathogenic bacteria, such as Burkholderia, Rhodococcus, Chlamydiae and Anaplasmataceae spp. (Neorickettsia helminthoeca and Ehrlichia ovina). We also identified Wolbachia pipientis in a single beetle. This bacterium is a causative agent of reproductive alterations in arthropods. These results support the enemy release hypothesis in the case of this ladybird invasion. Pathogenic bacteria were recorded in only a few samples. Moreover, male-killing bacteria such as Spiroplasma spp., Wolbachia spp. and Rickettsia spp. were only recorded in single insects so they cannot be responsible for the observed alterations in the sex-ratio of the ladybird population studied.https://www.eje.cz/artkey/eje-201701-0038_Metagenomic_survey_of_bacteria_associated_with_the_invasive_ladybird_Harmonia_axyridis_Coleoptera_Coccinellid.phpcoleopteracoccinellidaeharmonia axyridismicrobiotabacteria community16s rnainsect symbionts |
| spellingShingle | Krzysztof DUDEK Kinga HUMIŃSKA Jacek WOJCIECHOWICZ Piotr TRYJANOWSKI Metagenomic survey of bacteria associated with the invasive ladybird Harmonia axyridis (Coleoptera: Coccinellidae) European Journal of Entomology coleoptera coccinellidae harmonia axyridis microbiota bacteria community 16s rna insect symbionts |
| title | Metagenomic survey of bacteria associated with the invasive ladybird Harmonia axyridis (Coleoptera: Coccinellidae) |
| title_full | Metagenomic survey of bacteria associated with the invasive ladybird Harmonia axyridis (Coleoptera: Coccinellidae) |
| title_fullStr | Metagenomic survey of bacteria associated with the invasive ladybird Harmonia axyridis (Coleoptera: Coccinellidae) |
| title_full_unstemmed | Metagenomic survey of bacteria associated with the invasive ladybird Harmonia axyridis (Coleoptera: Coccinellidae) |
| title_short | Metagenomic survey of bacteria associated with the invasive ladybird Harmonia axyridis (Coleoptera: Coccinellidae) |
| title_sort | metagenomic survey of bacteria associated with the invasive ladybird harmonia axyridis coleoptera coccinellidae |
| topic | coleoptera coccinellidae harmonia axyridis microbiota bacteria community 16s rna insect symbionts |
| url | https://www.eje.cz/artkey/eje-201701-0038_Metagenomic_survey_of_bacteria_associated_with_the_invasive_ladybird_Harmonia_axyridis_Coleoptera_Coccinellid.php |
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