Identification of Mosquito Bloodmeals Collected in Diverse Habitats in Malaysian Borneo Using COI Barcoding
Land cover and land use change (LCLUC) acts as a catalyst for spillover of arthropod-borne pathogens into novel hosts by shifting host and vector diversity, abundance, and distribution, ultimately reshaping host–vector interactions. Identification of bloodmeals from wild-caught mosquitoes provides i...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | Tropical Medicine and Infectious Disease |
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| Online Access: | https://www.mdpi.com/2414-6366/5/2/51 |
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| author | Katherine I. Young Joseph T. Medwid Sasha R. Azar Robert M. Huff Hannah Drumm Lark L. Coffey R. Jason Pitts Michaela Buenemann Nikos Vasilakis David Perera Kathryn A. Hanley |
| author_facet | Katherine I. Young Joseph T. Medwid Sasha R. Azar Robert M. Huff Hannah Drumm Lark L. Coffey R. Jason Pitts Michaela Buenemann Nikos Vasilakis David Perera Kathryn A. Hanley |
| author_sort | Katherine I. Young |
| collection | DOAJ |
| description | Land cover and land use change (LCLUC) acts as a catalyst for spillover of arthropod-borne pathogens into novel hosts by shifting host and vector diversity, abundance, and distribution, ultimately reshaping host–vector interactions. Identification of bloodmeals from wild-caught mosquitoes provides insight into host utilization of particular species in particular land cover types, and hence their potential role in pathogen maintenance and spillover. Here, we collected 134 blood-engorged mosquitoes comprising 10 taxa across 9 land cover types in Sarawak, Malaysian Borneo, a region experiencing intense LCLUC and concomitant spillover of arthropod-borne pathogens. Host sources of blood were successfully identified for 116 (87%) mosquitoes using cytochrome oxidase subunit I (COI) barcoding. A diverse range of hosts were identified, including reptiles, amphibians, birds, and mammals. Sixteen engorged <i>Aedes albopictus</i>, a major vector of dengue virus, were collected from seven land cover types and found to feed exclusively on humans (73%) and boar (27%). <i>Culex tritaeniohynchus</i> (n = 2), <i>Cx. gelidus</i> (n = 3), and <i>Cx. quiquefasciatus</i> (n = 3), vectors of Japanese encephalitis virus, fed on humans and pigs in the rural built-up land cover, creating potential transmission networks between these species. Our data support the use of COI barcoding to characterize mosquito–host networks in a biodiversity hotspot. |
| first_indexed | 2024-03-10T20:44:44Z |
| format | Article |
| id | doaj.art-38318e65cc7541de87d3da5ab81fe2aa |
| institution | Directory Open Access Journal |
| issn | 2414-6366 |
| language | English |
| last_indexed | 2024-03-10T20:44:44Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Tropical Medicine and Infectious Disease |
| spelling | doaj.art-38318e65cc7541de87d3da5ab81fe2aa2023-11-19T20:23:20ZengMDPI AGTropical Medicine and Infectious Disease2414-63662020-04-01525110.3390/tropicalmed5020051Identification of Mosquito Bloodmeals Collected in Diverse Habitats in Malaysian Borneo Using COI BarcodingKatherine I. Young0Joseph T. Medwid1Sasha R. Azar2Robert M. Huff3Hannah Drumm4Lark L. Coffey5R. Jason Pitts6Michaela Buenemann7Nikos Vasilakis8David Perera9Kathryn A. Hanley10Department of Biology, New Mexico State University, Las Cruces NM 88003, USADepartment of Biology, New Mexico State University, Las Cruces NM 88003, USADepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USADepartment of Biology, Baylor University, Waco, TX 76706, USASchool of Veterinary Medicine, University of California Davis, Davis, CA 95616, USASchool of Veterinary Medicine, University of California Davis, Davis, CA 95616, USADepartment of Biology, Baylor University, Waco, TX 76706, USADepartment of Geography, New Mexico State University, Las Cruces, NM 88003, USADepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USAInstitute of Health and Communiti Medicine, Universiti of Malaysia Sarawak, Sarawak 94300, MalaysiaDepartment of Biology, New Mexico State University, Las Cruces NM 88003, USALand cover and land use change (LCLUC) acts as a catalyst for spillover of arthropod-borne pathogens into novel hosts by shifting host and vector diversity, abundance, and distribution, ultimately reshaping host–vector interactions. Identification of bloodmeals from wild-caught mosquitoes provides insight into host utilization of particular species in particular land cover types, and hence their potential role in pathogen maintenance and spillover. Here, we collected 134 blood-engorged mosquitoes comprising 10 taxa across 9 land cover types in Sarawak, Malaysian Borneo, a region experiencing intense LCLUC and concomitant spillover of arthropod-borne pathogens. Host sources of blood were successfully identified for 116 (87%) mosquitoes using cytochrome oxidase subunit I (COI) barcoding. A diverse range of hosts were identified, including reptiles, amphibians, birds, and mammals. Sixteen engorged <i>Aedes albopictus</i>, a major vector of dengue virus, were collected from seven land cover types and found to feed exclusively on humans (73%) and boar (27%). <i>Culex tritaeniohynchus</i> (n = 2), <i>Cx. gelidus</i> (n = 3), and <i>Cx. quiquefasciatus</i> (n = 3), vectors of Japanese encephalitis virus, fed on humans and pigs in the rural built-up land cover, creating potential transmission networks between these species. Our data support the use of COI barcoding to characterize mosquito–host networks in a biodiversity hotspot.https://www.mdpi.com/2414-6366/5/2/51mosquitovectorhostbloodmealarbovirusBorneo |
| spellingShingle | Katherine I. Young Joseph T. Medwid Sasha R. Azar Robert M. Huff Hannah Drumm Lark L. Coffey R. Jason Pitts Michaela Buenemann Nikos Vasilakis David Perera Kathryn A. Hanley Identification of Mosquito Bloodmeals Collected in Diverse Habitats in Malaysian Borneo Using COI Barcoding Tropical Medicine and Infectious Disease mosquito vector host bloodmeal arbovirus Borneo |
| title | Identification of Mosquito Bloodmeals Collected in Diverse Habitats in Malaysian Borneo Using COI Barcoding |
| title_full | Identification of Mosquito Bloodmeals Collected in Diverse Habitats in Malaysian Borneo Using COI Barcoding |
| title_fullStr | Identification of Mosquito Bloodmeals Collected in Diverse Habitats in Malaysian Borneo Using COI Barcoding |
| title_full_unstemmed | Identification of Mosquito Bloodmeals Collected in Diverse Habitats in Malaysian Borneo Using COI Barcoding |
| title_short | Identification of Mosquito Bloodmeals Collected in Diverse Habitats in Malaysian Borneo Using COI Barcoding |
| title_sort | identification of mosquito bloodmeals collected in diverse habitats in malaysian borneo using coi barcoding |
| topic | mosquito vector host bloodmeal arbovirus Borneo |
| url | https://www.mdpi.com/2414-6366/5/2/51 |
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