Galanin-Mediated Behavioural Hyperalgesia from the Dorsomedial Nucleus of the Hypothalamus Involves Two Independent Descending Pronociceptive Pathways.
Activation of the dorsomedial nucleus of the hypothalamus (DMH) by galanin (GAL) induces behavioural hyperalgesia. Since DMH neurones do not project directly to the spinal cord, we hypothesized that the medullary dorsal reticular nucleus (DRt), a pronociceptive region projecting to the spinal dorsal...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4643915?pdf=render |
| _version_ | 1811320307382747136 |
|---|---|
| author | Diana Amorim Hanna Viisanen Hong Wei Armando Almeida Antti Pertovaara Filipa Pinto-Ribeiro |
| author_facet | Diana Amorim Hanna Viisanen Hong Wei Armando Almeida Antti Pertovaara Filipa Pinto-Ribeiro |
| author_sort | Diana Amorim |
| collection | DOAJ |
| description | Activation of the dorsomedial nucleus of the hypothalamus (DMH) by galanin (GAL) induces behavioural hyperalgesia. Since DMH neurones do not project directly to the spinal cord, we hypothesized that the medullary dorsal reticular nucleus (DRt), a pronociceptive region projecting to the spinal dorsal horn (SDH) and/or the serotoninergic raphe-spinal pathway acting on the spinal 5-HT3 receptor (5HT3R) could relay descending nociceptive facilitation induced by GAL in the DMH. Heat-evoked paw-withdrawal latency (PWL) and activity of SDH neurones were assessed in monoarthritic (ARTH) and control (SHAM) animals after pharmacological manipulations of the DMH, DRt and spinal cord. The results showed that GAL in the DMH and glutamate in the DRt lead to behavioural hyperalgesia in both SHAM and ARTH animals, which is accompanied particularly by an increase in heat-evoked responses of wide-dynamic range neurons, a group of nociceptive SDH neurones. Facilitation of pain behaviour induced by GAL in the DMH was reversed by lidocaine in the DRt and by ondansetron, a 5HT3R antagonist, in the spinal cord. However, the hyperalgesia induced by glutamate in the DRt was not blocked by spinal ondansetron. In addition, in ARTH but not SHAM animals PWL was increased after lidocaine in the DRt and ondansetron in the spinal cord. Our data demonstrate that GAL in the DMH activates two independent descending facilitatory pathways: (i) one relays in the DRt and (ii) the other one involves 5-HT neurones acting on spinal 5HT3Rs. In experimental ARTH, the tonic pain-facilitatory action is increased in both of these descending pathways. |
| first_indexed | 2024-04-13T12:57:31Z |
| format | Article |
| id | doaj.art-3832c898a81142deadb1704935796bdc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T12:57:31Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3832c898a81142deadb1704935796bdc2022-12-22T02:46:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011011e014291910.1371/journal.pone.0142919Galanin-Mediated Behavioural Hyperalgesia from the Dorsomedial Nucleus of the Hypothalamus Involves Two Independent Descending Pronociceptive Pathways.Diana AmorimHanna ViisanenHong WeiArmando AlmeidaAntti PertovaaraFilipa Pinto-RibeiroActivation of the dorsomedial nucleus of the hypothalamus (DMH) by galanin (GAL) induces behavioural hyperalgesia. Since DMH neurones do not project directly to the spinal cord, we hypothesized that the medullary dorsal reticular nucleus (DRt), a pronociceptive region projecting to the spinal dorsal horn (SDH) and/or the serotoninergic raphe-spinal pathway acting on the spinal 5-HT3 receptor (5HT3R) could relay descending nociceptive facilitation induced by GAL in the DMH. Heat-evoked paw-withdrawal latency (PWL) and activity of SDH neurones were assessed in monoarthritic (ARTH) and control (SHAM) animals after pharmacological manipulations of the DMH, DRt and spinal cord. The results showed that GAL in the DMH and glutamate in the DRt lead to behavioural hyperalgesia in both SHAM and ARTH animals, which is accompanied particularly by an increase in heat-evoked responses of wide-dynamic range neurons, a group of nociceptive SDH neurones. Facilitation of pain behaviour induced by GAL in the DMH was reversed by lidocaine in the DRt and by ondansetron, a 5HT3R antagonist, in the spinal cord. However, the hyperalgesia induced by glutamate in the DRt was not blocked by spinal ondansetron. In addition, in ARTH but not SHAM animals PWL was increased after lidocaine in the DRt and ondansetron in the spinal cord. Our data demonstrate that GAL in the DMH activates two independent descending facilitatory pathways: (i) one relays in the DRt and (ii) the other one involves 5-HT neurones acting on spinal 5HT3Rs. In experimental ARTH, the tonic pain-facilitatory action is increased in both of these descending pathways.http://europepmc.org/articles/PMC4643915?pdf=render |
| spellingShingle | Diana Amorim Hanna Viisanen Hong Wei Armando Almeida Antti Pertovaara Filipa Pinto-Ribeiro Galanin-Mediated Behavioural Hyperalgesia from the Dorsomedial Nucleus of the Hypothalamus Involves Two Independent Descending Pronociceptive Pathways. PLoS ONE |
| title | Galanin-Mediated Behavioural Hyperalgesia from the Dorsomedial Nucleus of the Hypothalamus Involves Two Independent Descending Pronociceptive Pathways. |
| title_full | Galanin-Mediated Behavioural Hyperalgesia from the Dorsomedial Nucleus of the Hypothalamus Involves Two Independent Descending Pronociceptive Pathways. |
| title_fullStr | Galanin-Mediated Behavioural Hyperalgesia from the Dorsomedial Nucleus of the Hypothalamus Involves Two Independent Descending Pronociceptive Pathways. |
| title_full_unstemmed | Galanin-Mediated Behavioural Hyperalgesia from the Dorsomedial Nucleus of the Hypothalamus Involves Two Independent Descending Pronociceptive Pathways. |
| title_short | Galanin-Mediated Behavioural Hyperalgesia from the Dorsomedial Nucleus of the Hypothalamus Involves Two Independent Descending Pronociceptive Pathways. |
| title_sort | galanin mediated behavioural hyperalgesia from the dorsomedial nucleus of the hypothalamus involves two independent descending pronociceptive pathways |
| url | http://europepmc.org/articles/PMC4643915?pdf=render |
| work_keys_str_mv | AT dianaamorim galaninmediatedbehaviouralhyperalgesiafromthedorsomedialnucleusofthehypothalamusinvolvestwoindependentdescendingpronociceptivepathways AT hannaviisanen galaninmediatedbehaviouralhyperalgesiafromthedorsomedialnucleusofthehypothalamusinvolvestwoindependentdescendingpronociceptivepathways AT hongwei galaninmediatedbehaviouralhyperalgesiafromthedorsomedialnucleusofthehypothalamusinvolvestwoindependentdescendingpronociceptivepathways AT armandoalmeida galaninmediatedbehaviouralhyperalgesiafromthedorsomedialnucleusofthehypothalamusinvolvestwoindependentdescendingpronociceptivepathways AT anttipertovaara galaninmediatedbehaviouralhyperalgesiafromthedorsomedialnucleusofthehypothalamusinvolvestwoindependentdescendingpronociceptivepathways AT filipapintoribeiro galaninmediatedbehaviouralhyperalgesiafromthedorsomedialnucleusofthehypothalamusinvolvestwoindependentdescendingpronociceptivepathways |