Establishment of a Rapid Plant Regeneration System in <i>Physalis angulata</i> L. through Axillary Meristems
<p>An optimal plant propagation method of <em>Physalis angulata</em> L., a medicinally important herbaceous plant species has been developed using axillary meristem explants. Shoot bud proliferation was initiated from axillary meristem explants cultured on MS medium supplemented wi...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2015-12-01
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| Series: | Notulae Scientia Biologicae |
| Online Access: | http://notulaebiologicae.ro/index.php/nsb/article/view/9707 |
| _version_ | 1818491716130308096 |
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| author | Owk ANIEL KUMAR Songa RAMESH Sape SUBBA TATA |
| author_facet | Owk ANIEL KUMAR Songa RAMESH Sape SUBBA TATA |
| author_sort | Owk ANIEL KUMAR |
| collection | DOAJ |
| description | <p>An optimal plant propagation method of <em>Physalis angulata</em> L., a medicinally important herbaceous plant species has been developed using axillary meristem explants. Shoot bud proliferation was initiated from axillary meristem explants cultured on MS medium supplemented with various concentrations of 0.5-2.5mg/L/(BAP)/(Zeatin)/(KIN). The maximum <em>in vitro</em> response of shooting frequency of explants (88.1%) and shoots per explant (42) was achieved with medium containing 1.0mg/L BAP. Multiple shoot culture was established by repeated subculturing of the shoot buds of axillary meristems on shoot multiplication medium. Among the subculture media BAP in combination with 1.5mg/L (IAA)+0.25mg/L(GA<sub>3</sub>) produced maximum shoots per explant (128±0.29) after two weeks of culture. Effective <em>in vitro </em>shoot elongation and rooting was achieved on 1.0mg/L(GA<sub>3</sub>) and 1.0mg/L(IBA), respectively. Most of the generated shoots were successfully transferred to soil under field conditions. The survival percentage of the transferred plants on soil was found to be 90 per cent. This protocol can be used for commercial propagation and for future genetic improvement studies.</p> |
| first_indexed | 2024-12-10T17:34:40Z |
| format | Article |
| id | doaj.art-383420f8ce454c46833ce3c3aa07161c |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-12-10T17:34:40Z |
| publishDate | 2015-12-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-383420f8ce454c46833ce3c3aa07161c2022-12-22T01:39:35ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642015-12-017447147410.15835/nsb.7.4.97078188Establishment of a Rapid Plant Regeneration System in <i>Physalis angulata</i> L. through Axillary MeristemsOwk ANIEL KUMAR0Songa RAMESH1Sape SUBBA TATA2Andhra University, Department of Botany, VisakhapatnamAndhra University, Department of Botany, VisakhapatnamAndhra University, Department of Botany, Visakhapatnam<p>An optimal plant propagation method of <em>Physalis angulata</em> L., a medicinally important herbaceous plant species has been developed using axillary meristem explants. Shoot bud proliferation was initiated from axillary meristem explants cultured on MS medium supplemented with various concentrations of 0.5-2.5mg/L/(BAP)/(Zeatin)/(KIN). The maximum <em>in vitro</em> response of shooting frequency of explants (88.1%) and shoots per explant (42) was achieved with medium containing 1.0mg/L BAP. Multiple shoot culture was established by repeated subculturing of the shoot buds of axillary meristems on shoot multiplication medium. Among the subculture media BAP in combination with 1.5mg/L (IAA)+0.25mg/L(GA<sub>3</sub>) produced maximum shoots per explant (128±0.29) after two weeks of culture. Effective <em>in vitro </em>shoot elongation and rooting was achieved on 1.0mg/L(GA<sub>3</sub>) and 1.0mg/L(IBA), respectively. Most of the generated shoots were successfully transferred to soil under field conditions. The survival percentage of the transferred plants on soil was found to be 90 per cent. This protocol can be used for commercial propagation and for future genetic improvement studies.</p>http://notulaebiologicae.ro/index.php/nsb/article/view/9707 |
| spellingShingle | Owk ANIEL KUMAR Songa RAMESH Sape SUBBA TATA Establishment of a Rapid Plant Regeneration System in <i>Physalis angulata</i> L. through Axillary Meristems Notulae Scientia Biologicae |
| title | Establishment of a Rapid Plant Regeneration System in <i>Physalis angulata</i> L. through Axillary Meristems |
| title_full | Establishment of a Rapid Plant Regeneration System in <i>Physalis angulata</i> L. through Axillary Meristems |
| title_fullStr | Establishment of a Rapid Plant Regeneration System in <i>Physalis angulata</i> L. through Axillary Meristems |
| title_full_unstemmed | Establishment of a Rapid Plant Regeneration System in <i>Physalis angulata</i> L. through Axillary Meristems |
| title_short | Establishment of a Rapid Plant Regeneration System in <i>Physalis angulata</i> L. through Axillary Meristems |
| title_sort | establishment of a rapid plant regeneration system in i physalis angulata i l through axillary meristems |
| url | http://notulaebiologicae.ro/index.php/nsb/article/view/9707 |
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