Repetitive Noninvasive Monitoring of HSV1-tk-Expressing T Cells Intravenously Infused into Nonhuman Primates Using Positron Emission Tomography and Computed Tomography with F-FEAU
Adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) has been successfully used to treat patients with different types of cancer. However, the long-term spatial-temporal dynamics of the distribution of systemically infused CTLs remains largely unknown. Noninvasive imaging of adoptive...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publications
2009-07-01
|
| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2009.00022 |
| _version_ | 1797282206110973952 |
|---|---|
| author | Gianpietro Dotti Mei Tian Barbara Savoldo Amer Najjar Laurence J.N. Cooper James Jackson Amanda Smith Osama Mawlawi Rajesh Uthamanthil Agatha Borne David Brammer Vincenzo Paolillo Mian Alauddin Carlos Gonzalez David Steiner William K. Decker Frank Marini Steven Kornblau Catherine M. Bollard Elizabeth J. Shpall Juri G. Gelovani |
| author_facet | Gianpietro Dotti Mei Tian Barbara Savoldo Amer Najjar Laurence J.N. Cooper James Jackson Amanda Smith Osama Mawlawi Rajesh Uthamanthil Agatha Borne David Brammer Vincenzo Paolillo Mian Alauddin Carlos Gonzalez David Steiner William K. Decker Frank Marini Steven Kornblau Catherine M. Bollard Elizabeth J. Shpall Juri G. Gelovani |
| author_sort | Gianpietro Dotti |
| collection | DOAJ |
| description | Adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) has been successfully used to treat patients with different types of cancer. However, the long-term spatial-temporal dynamics of the distribution of systemically infused CTLs remains largely unknown. Noninvasive imaging of adoptively transferred CTLs using molecular-genetic reporter imaging with positron emission tomography and computed tomography (PET-CT) represents an innovative approach to understanding the long-term migratory patterns and therapeutic potential of adoptively transferred T cells. Here we report the application of repetitive PET-CT imaging with [ 18 F]fluoro-5-ethyl-1-beta-D-arabinofuranosyluracil ( 18 F-FEAU) in two nonhuman primates demonstrating that autologous polyclonal macaque T lymphocytes activated and transduced with a retroviral vector encoding for the sr39 mutant herpes simplex virus 1 thymidine kinase ( sr39HSV1-tk ) reporter gene can be detected after intravenous infusion in discrete lymphoid organs and in sites of inflammation. This study represents a proof of principle and supports the application of 18 F-FEAU PET-CT imaging for monitoring the distribution of intravenously administered sr39HSV1-tk gene-transduced CTLs in humans. |
| first_indexed | 2024-03-07T17:09:37Z |
| format | Article |
| id | doaj.art-3835762226744d6893724234d06f864d |
| institution | Directory Open Access Journal |
| issn | 1536-0121 |
| language | English |
| last_indexed | 2024-03-07T17:09:37Z |
| publishDate | 2009-07-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj.art-3835762226744d6893724234d06f864d2024-03-03T01:20:41ZengSAGE PublicationsMolecular Imaging1536-01212009-07-01810.2310/7290.2009.0002210.2310_7290.2009.00022Repetitive Noninvasive Monitoring of HSV1-tk-Expressing T Cells Intravenously Infused into Nonhuman Primates Using Positron Emission Tomography and Computed Tomography with F-FEAUGianpietro DottiMei TianBarbara SavoldoAmer NajjarLaurence J.N. CooperJames JacksonAmanda SmithOsama MawlawiRajesh UthamanthilAgatha BorneDavid BrammerVincenzo PaolilloMian AlauddinCarlos GonzalezDavid SteinerWilliam K. DeckerFrank MariniSteven KornblauCatherine M. BollardElizabeth J. ShpallJuri G. GelovaniAdoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) has been successfully used to treat patients with different types of cancer. However, the long-term spatial-temporal dynamics of the distribution of systemically infused CTLs remains largely unknown. Noninvasive imaging of adoptively transferred CTLs using molecular-genetic reporter imaging with positron emission tomography and computed tomography (PET-CT) represents an innovative approach to understanding the long-term migratory patterns and therapeutic potential of adoptively transferred T cells. Here we report the application of repetitive PET-CT imaging with [ 18 F]fluoro-5-ethyl-1-beta-D-arabinofuranosyluracil ( 18 F-FEAU) in two nonhuman primates demonstrating that autologous polyclonal macaque T lymphocytes activated and transduced with a retroviral vector encoding for the sr39 mutant herpes simplex virus 1 thymidine kinase ( sr39HSV1-tk ) reporter gene can be detected after intravenous infusion in discrete lymphoid organs and in sites of inflammation. This study represents a proof of principle and supports the application of 18 F-FEAU PET-CT imaging for monitoring the distribution of intravenously administered sr39HSV1-tk gene-transduced CTLs in humans.https://doi.org/10.2310/7290.2009.00022 |
| spellingShingle | Gianpietro Dotti Mei Tian Barbara Savoldo Amer Najjar Laurence J.N. Cooper James Jackson Amanda Smith Osama Mawlawi Rajesh Uthamanthil Agatha Borne David Brammer Vincenzo Paolillo Mian Alauddin Carlos Gonzalez David Steiner William K. Decker Frank Marini Steven Kornblau Catherine M. Bollard Elizabeth J. Shpall Juri G. Gelovani Repetitive Noninvasive Monitoring of HSV1-tk-Expressing T Cells Intravenously Infused into Nonhuman Primates Using Positron Emission Tomography and Computed Tomography with F-FEAU Molecular Imaging |
| title | Repetitive Noninvasive Monitoring of HSV1-tk-Expressing T Cells Intravenously Infused into Nonhuman Primates Using Positron Emission Tomography and Computed Tomography with F-FEAU |
| title_full | Repetitive Noninvasive Monitoring of HSV1-tk-Expressing T Cells Intravenously Infused into Nonhuman Primates Using Positron Emission Tomography and Computed Tomography with F-FEAU |
| title_fullStr | Repetitive Noninvasive Monitoring of HSV1-tk-Expressing T Cells Intravenously Infused into Nonhuman Primates Using Positron Emission Tomography and Computed Tomography with F-FEAU |
| title_full_unstemmed | Repetitive Noninvasive Monitoring of HSV1-tk-Expressing T Cells Intravenously Infused into Nonhuman Primates Using Positron Emission Tomography and Computed Tomography with F-FEAU |
| title_short | Repetitive Noninvasive Monitoring of HSV1-tk-Expressing T Cells Intravenously Infused into Nonhuman Primates Using Positron Emission Tomography and Computed Tomography with F-FEAU |
| title_sort | repetitive noninvasive monitoring of hsv1 tk expressing t cells intravenously infused into nonhuman primates using positron emission tomography and computed tomography with f feau |
| url | https://doi.org/10.2310/7290.2009.00022 |
| work_keys_str_mv | AT gianpietrodotti repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT meitian repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT barbarasavoldo repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT amernajjar repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT laurencejncooper repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT jamesjackson repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT amandasmith repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT osamamawlawi repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT rajeshuthamanthil repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT agathaborne repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT davidbrammer repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT vincenzopaolillo repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT mianalauddin repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT carlosgonzalez repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT davidsteiner repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT williamkdecker repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT frankmarini repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT stevenkornblau repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT catherinembollard repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT elizabethjshpall repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau AT juriggelovani repetitivenoninvasivemonitoringofhsv1tkexpressingtcellsintravenouslyinfusedintononhumanprimatesusingpositronemissiontomographyandcomputedtomographywithffeau |