Parasitization of <i>Aphis gossypii</i> Glover by <i>Binodoxys communis</i> Gahan Causes Shifts in the Ovarian Bacterial Microbiota
Background: <i>Aphis gossypii</i> Glover is an important agricultural pest distributed worldwide. <i>Binodoxys communis</i> Gahan is the main parasitoid wasp of <i>A. gossypii</i>. Previous studies have shown that parasitization causes reduced egg production in &l...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/4/314 |
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| author | Jinming Li Zhe An Junyu Luo Xiangzhen Zhu Li Wang Kaixin Zhang Dongyang Li Jichao Ji Lin Niu Xueke Gao Jinjie Cui |
| author_facet | Jinming Li Zhe An Junyu Luo Xiangzhen Zhu Li Wang Kaixin Zhang Dongyang Li Jichao Ji Lin Niu Xueke Gao Jinjie Cui |
| author_sort | Jinming Li |
| collection | DOAJ |
| description | Background: <i>Aphis gossypii</i> Glover is an important agricultural pest distributed worldwide. <i>Binodoxys communis</i> Gahan is the main parasitoid wasp of <i>A. gossypii</i>. Previous studies have shown that parasitization causes reduced egg production in <i>A. gossypii</i>, but the effects of parasitism on the symbiotic bacteria in the host ovaries are unknown. Results: In this study, we analyzed the microbial communities in the ovaries of <i>A. gossypii</i> without and after parasitization. Whether parasitized or not, <i>Buchnera</i> was the dominant genus of symbiotic bacteria in the ovaries, followed by facultative symbionts including <i>Arsenophonus</i>, <i>Pseudomonas</i>, and <i>Acinetobacter</i>. The relative abundance of <i>Buchnera</i> in the aphid ovary increased after parasitization for 1 d in both third-instar nymph and adult stages, but decreased after parasitization for 3 d. The shifts in the relative abundance of <i>Arsenophonus</i> in both stages were the same as those observed for <i>Buchnera.</i> In addition, the relative abundance of <i>Serratia</i> remarkably decreased after parasitization for 1 d and increased after parasitization for 3 d. A functional predictive analysis of the control and parasitized ovary microbiomes revealed that pathways primarily enriched in parasitization were “amino acid transport and metabolism” and “energy production and conversion.” Finally, RT-qPCR analysis was performed on <i>Buchnera</i>, <i>Arsenophonus</i>, and <i>Serratia</i>. The results of RT-qPCR were the same as the results of 16S rDNA sequencing. Conclusions: These results provide a framework for investigating shifts in the microbial communities in host ovaries, which may be responsible for reduced egg production in aphids. These findings also broaden our understanding of the interactions among aphids, parasitoid wasps, and endosymbionts. |
| first_indexed | 2024-03-11T04:54:52Z |
| format | Article |
| id | doaj.art-38391df6ca164b22b1153dac1e3b536d |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T04:54:52Z |
| publishDate | 2023-03-01 |
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| series | Insects |
| spelling | doaj.art-38391df6ca164b22b1153dac1e3b536d2023-11-17T19:45:48ZengMDPI AGInsects2075-44502023-03-0114431410.3390/insects14040314Parasitization of <i>Aphis gossypii</i> Glover by <i>Binodoxys communis</i> Gahan Causes Shifts in the Ovarian Bacterial MicrobiotaJinming Li0Zhe An1Junyu Luo2Xiangzhen Zhu3Li Wang4Kaixin Zhang5Dongyang Li6Jichao Ji7Lin Niu8Xueke Gao9Jinjie Cui10Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaBackground: <i>Aphis gossypii</i> Glover is an important agricultural pest distributed worldwide. <i>Binodoxys communis</i> Gahan is the main parasitoid wasp of <i>A. gossypii</i>. Previous studies have shown that parasitization causes reduced egg production in <i>A. gossypii</i>, but the effects of parasitism on the symbiotic bacteria in the host ovaries are unknown. Results: In this study, we analyzed the microbial communities in the ovaries of <i>A. gossypii</i> without and after parasitization. Whether parasitized or not, <i>Buchnera</i> was the dominant genus of symbiotic bacteria in the ovaries, followed by facultative symbionts including <i>Arsenophonus</i>, <i>Pseudomonas</i>, and <i>Acinetobacter</i>. The relative abundance of <i>Buchnera</i> in the aphid ovary increased after parasitization for 1 d in both third-instar nymph and adult stages, but decreased after parasitization for 3 d. The shifts in the relative abundance of <i>Arsenophonus</i> in both stages were the same as those observed for <i>Buchnera.</i> In addition, the relative abundance of <i>Serratia</i> remarkably decreased after parasitization for 1 d and increased after parasitization for 3 d. A functional predictive analysis of the control and parasitized ovary microbiomes revealed that pathways primarily enriched in parasitization were “amino acid transport and metabolism” and “energy production and conversion.” Finally, RT-qPCR analysis was performed on <i>Buchnera</i>, <i>Arsenophonus</i>, and <i>Serratia</i>. The results of RT-qPCR were the same as the results of 16S rDNA sequencing. Conclusions: These results provide a framework for investigating shifts in the microbial communities in host ovaries, which may be responsible for reduced egg production in aphids. These findings also broaden our understanding of the interactions among aphids, parasitoid wasps, and endosymbionts.https://www.mdpi.com/2075-4450/14/4/314parasitoids<i>Buchnera</i><i>Serratia</i><i>Arsenophonus</i>host–parasite interactionsaphid |
| spellingShingle | Jinming Li Zhe An Junyu Luo Xiangzhen Zhu Li Wang Kaixin Zhang Dongyang Li Jichao Ji Lin Niu Xueke Gao Jinjie Cui Parasitization of <i>Aphis gossypii</i> Glover by <i>Binodoxys communis</i> Gahan Causes Shifts in the Ovarian Bacterial Microbiota Insects parasitoids <i>Buchnera</i> <i>Serratia</i> <i>Arsenophonus</i> host–parasite interactions aphid |
| title | Parasitization of <i>Aphis gossypii</i> Glover by <i>Binodoxys communis</i> Gahan Causes Shifts in the Ovarian Bacterial Microbiota |
| title_full | Parasitization of <i>Aphis gossypii</i> Glover by <i>Binodoxys communis</i> Gahan Causes Shifts in the Ovarian Bacterial Microbiota |
| title_fullStr | Parasitization of <i>Aphis gossypii</i> Glover by <i>Binodoxys communis</i> Gahan Causes Shifts in the Ovarian Bacterial Microbiota |
| title_full_unstemmed | Parasitization of <i>Aphis gossypii</i> Glover by <i>Binodoxys communis</i> Gahan Causes Shifts in the Ovarian Bacterial Microbiota |
| title_short | Parasitization of <i>Aphis gossypii</i> Glover by <i>Binodoxys communis</i> Gahan Causes Shifts in the Ovarian Bacterial Microbiota |
| title_sort | parasitization of i aphis gossypii i glover by i binodoxys communis i gahan causes shifts in the ovarian bacterial microbiota |
| topic | parasitoids <i>Buchnera</i> <i>Serratia</i> <i>Arsenophonus</i> host–parasite interactions aphid |
| url | https://www.mdpi.com/2075-4450/14/4/314 |
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