Hierarchical Action of Mulberry miR156 in the Vegetative Phase Transition
The vegetative phase transition is a prerequisite for flowering in angiosperm plants. Mulberry miR156 has been confirmed to be a crucial factor in the vegetative phase transition in <i>Arabidopsis thaliana</i>. The over-expression of miR156 in transgenic <i>Populus × canadensis<...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/11/5550 |
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| author | Hongshun Li Yiwei Luo Bi Ma Jianqiong Hu Zhiyuan Lv Wuqi Wei Haiye Hao Jianglian Yuan Ningjia He |
| author_facet | Hongshun Li Yiwei Luo Bi Ma Jianqiong Hu Zhiyuan Lv Wuqi Wei Haiye Hao Jianglian Yuan Ningjia He |
| author_sort | Hongshun Li |
| collection | DOAJ |
| description | The vegetative phase transition is a prerequisite for flowering in angiosperm plants. Mulberry miR156 has been confirmed to be a crucial factor in the vegetative phase transition in <i>Arabidopsis thaliana</i>. The over-expression of miR156 in transgenic <i>Populus × canadensis</i> dramatically prolongs the juvenile phase. Here, we find that the expression of mno-miR156 decreases with age in all tissues in mulberry, which led us to study the hierarchical action of miR156 in mulberry. Utilizing degradome sequencing and dual-luciferase reporter assays, nine <i>MnSPL</i>s were shown to be directly regulated by miR156. The results of yeast one-hybrid and dual-luciferase reporter assays also revealed that six MnSPLs could recognize the promoter sequences of mno-miR172 and activate its expression. Our results demonstrate that mno-miR156 performs its role by repressing MnSPL/mno-miR172 pathway expression in mulberry. This work uncovered a miR156/SPLs/miR172 regulation pathway in the development of mulberry and fills a gap in our knowledge about the molecular mechanism of vegetative phase transition in perennial woody plants. |
| first_indexed | 2024-03-10T11:05:13Z |
| format | Article |
| id | doaj.art-383b0ff4fcb9466c8bcc66bc68012021 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T11:05:13Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-383b0ff4fcb9466c8bcc66bc680120212023-11-21T21:11:57ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-012211555010.3390/ijms22115550Hierarchical Action of Mulberry miR156 in the Vegetative Phase TransitionHongshun Li0Yiwei Luo1Bi Ma2Jianqiong Hu3Zhiyuan Lv4Wuqi Wei5Haiye Hao6Jianglian Yuan7Ningjia He8State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, ChinaThe vegetative phase transition is a prerequisite for flowering in angiosperm plants. Mulberry miR156 has been confirmed to be a crucial factor in the vegetative phase transition in <i>Arabidopsis thaliana</i>. The over-expression of miR156 in transgenic <i>Populus × canadensis</i> dramatically prolongs the juvenile phase. Here, we find that the expression of mno-miR156 decreases with age in all tissues in mulberry, which led us to study the hierarchical action of miR156 in mulberry. Utilizing degradome sequencing and dual-luciferase reporter assays, nine <i>MnSPL</i>s were shown to be directly regulated by miR156. The results of yeast one-hybrid and dual-luciferase reporter assays also revealed that six MnSPLs could recognize the promoter sequences of mno-miR172 and activate its expression. Our results demonstrate that mno-miR156 performs its role by repressing MnSPL/mno-miR172 pathway expression in mulberry. This work uncovered a miR156/SPLs/miR172 regulation pathway in the development of mulberry and fills a gap in our knowledge about the molecular mechanism of vegetative phase transition in perennial woody plants.https://www.mdpi.com/1422-0067/22/11/5550age pathwaymir156smulberryperennial woody plantsvegetative phase transition |
| spellingShingle | Hongshun Li Yiwei Luo Bi Ma Jianqiong Hu Zhiyuan Lv Wuqi Wei Haiye Hao Jianglian Yuan Ningjia He Hierarchical Action of Mulberry miR156 in the Vegetative Phase Transition International Journal of Molecular Sciences age pathway mir156s mulberry perennial woody plants vegetative phase transition |
| title | Hierarchical Action of Mulberry miR156 in the Vegetative Phase Transition |
| title_full | Hierarchical Action of Mulberry miR156 in the Vegetative Phase Transition |
| title_fullStr | Hierarchical Action of Mulberry miR156 in the Vegetative Phase Transition |
| title_full_unstemmed | Hierarchical Action of Mulberry miR156 in the Vegetative Phase Transition |
| title_short | Hierarchical Action of Mulberry miR156 in the Vegetative Phase Transition |
| title_sort | hierarchical action of mulberry mir156 in the vegetative phase transition |
| topic | age pathway mir156s mulberry perennial woody plants vegetative phase transition |
| url | https://www.mdpi.com/1422-0067/22/11/5550 |
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