Nuciferine relieves type 2 diabetes mellitus via enhancing GLUT4 expression and translocation
Nuciferine contained in lotus leaves have been confirmed to have the effect of ameliorating hyperlipemia and hyperglycemia. A laser scanning confocal microscope was used to track the translocation of glucose transporter 4 (GLUT4) in L6 cells and the changes in intracellular Ca2+ levels in real time,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000721 |
| _version_ | 1797695758180286464 |
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| author | Tongxi Zhou Guanjun Song Di Tian Qinghua Liu Jinhua Shen Xinzhou Yang Ping Zhao |
| author_facet | Tongxi Zhou Guanjun Song Di Tian Qinghua Liu Jinhua Shen Xinzhou Yang Ping Zhao |
| author_sort | Tongxi Zhou |
| collection | DOAJ |
| description | Nuciferine contained in lotus leaves have been confirmed to have the effect of ameliorating hyperlipemia and hyperglycemia. A laser scanning confocal microscope was used to track the translocation of glucose transporter 4 (GLUT4) in L6 cells and the changes in intracellular Ca2+ levels in real time, and related protease inhibitors combined with western blotting were used to explore the mechanism of nuciferine. Meanwhile, KK-Ay mice, the spontaneous type 2 diabetic mice, were used to evaluate the in vivo activity of nuciferine. In this study, the in vitro studies indicated that nuciferine-induced GLUT4 translocation was regulated by G protein-PLC-PKC and AMPK pathways and nuciferine-enhanced intracellular Ca2+ was mediated by G protein-PLC-IP3-IP3R pathway, the increase in intracellular Ca2+ caused by nuciferine was not directly related to GLUT4 translocation, but both promote glucose uptake. The in vivo results suggested that nuciferine ameliorated weight gain induced by high-fat diet, abnormal lipid metabolism and the symptoms of insulin resistance in KK-Ay diabetic mice. Western blot results suggested that nuciferine increased AMPK and PKC phosphorylation levels in skeletal muscle and liver, and enhanced GLUT4 expression in skeletal muscle. Taken together, this research showed that nuciferine has the non-negligible potential in the treatment of type 2 diabetes mellitus. |
| first_indexed | 2024-03-12T03:15:54Z |
| format | Article |
| id | doaj.art-383ee2223103451683f7be57bf75a0a8 |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T03:15:54Z |
| publishDate | 2023-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-383ee2223103451683f7be57bf75a0a82023-09-03T14:10:25ZengTsinghua University PressFood Science and Human Wellness2213-45302023-11-0112620402051Nuciferine relieves type 2 diabetes mellitus via enhancing GLUT4 expression and translocationTongxi Zhou0Guanjun Song1Di Tian2Qinghua Liu3Jinhua Shen4Xinzhou Yang5Ping Zhao6School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, ChinaInstitute for Medical Biology & Hubei Provincial Key Laboratory for Protection and Application of Special Plants in the Wuling Area of China, College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, ChinaInstitute for Medical Biology & Hubei Provincial Key Laboratory for Protection and Application of Special Plants in the Wuling Area of China, College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, ChinaInstitute for Medical Biology & Hubei Provincial Key Laboratory for Protection and Application of Special Plants in the Wuling Area of China, College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, ChinaInstitute for Medical Biology & Hubei Provincial Key Laboratory for Protection and Application of Special Plants in the Wuling Area of China, College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, ChinaSchool of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, China; Corresponding authors.Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection and Application of Special Plants in the Wuling Area of China, College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, China; Corresponding authors.Nuciferine contained in lotus leaves have been confirmed to have the effect of ameliorating hyperlipemia and hyperglycemia. A laser scanning confocal microscope was used to track the translocation of glucose transporter 4 (GLUT4) in L6 cells and the changes in intracellular Ca2+ levels in real time, and related protease inhibitors combined with western blotting were used to explore the mechanism of nuciferine. Meanwhile, KK-Ay mice, the spontaneous type 2 diabetic mice, were used to evaluate the in vivo activity of nuciferine. In this study, the in vitro studies indicated that nuciferine-induced GLUT4 translocation was regulated by G protein-PLC-PKC and AMPK pathways and nuciferine-enhanced intracellular Ca2+ was mediated by G protein-PLC-IP3-IP3R pathway, the increase in intracellular Ca2+ caused by nuciferine was not directly related to GLUT4 translocation, but both promote glucose uptake. The in vivo results suggested that nuciferine ameliorated weight gain induced by high-fat diet, abnormal lipid metabolism and the symptoms of insulin resistance in KK-Ay diabetic mice. Western blot results suggested that nuciferine increased AMPK and PKC phosphorylation levels in skeletal muscle and liver, and enhanced GLUT4 expression in skeletal muscle. Taken together, this research showed that nuciferine has the non-negligible potential in the treatment of type 2 diabetes mellitus.http://www.sciencedirect.com/science/article/pii/S2213453023000721NuciferineType 2 diabetes mellitusglucose transporter 4 (GLUT4) |
| spellingShingle | Tongxi Zhou Guanjun Song Di Tian Qinghua Liu Jinhua Shen Xinzhou Yang Ping Zhao Nuciferine relieves type 2 diabetes mellitus via enhancing GLUT4 expression and translocation Food Science and Human Wellness Nuciferine Type 2 diabetes mellitus glucose transporter 4 (GLUT4) |
| title | Nuciferine relieves type 2 diabetes mellitus via enhancing GLUT4 expression and translocation |
| title_full | Nuciferine relieves type 2 diabetes mellitus via enhancing GLUT4 expression and translocation |
| title_fullStr | Nuciferine relieves type 2 diabetes mellitus via enhancing GLUT4 expression and translocation |
| title_full_unstemmed | Nuciferine relieves type 2 diabetes mellitus via enhancing GLUT4 expression and translocation |
| title_short | Nuciferine relieves type 2 diabetes mellitus via enhancing GLUT4 expression and translocation |
| title_sort | nuciferine relieves type 2 diabetes mellitus via enhancing glut4 expression and translocation |
| topic | Nuciferine Type 2 diabetes mellitus glucose transporter 4 (GLUT4) |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000721 |
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