The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia
Some studies on the hyperuricemia (HUA) have focused on intestinal bacteria. To better understand the correlation between gut microbiota and HUA, we established a HUA rat model with high-purine diet, and used 16S rRNA genes sequencing to analyze gut microbiota changes in HUA rats. To analyze the pot...
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PeerJ Inc.
2020-03-01
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| Online Access: | https://peerj.com/articles/8664.pdf |
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| author | Xiu Liu Qiulan Lv Hongyan Ren Liu Gao Peng Zhao Xiaomin Yang Guanpin Yang Daxing Xu Guangtao Wang Wan Yang Pengjun Wang Zenglan Wang Shichao Xing |
| author_facet | Xiu Liu Qiulan Lv Hongyan Ren Liu Gao Peng Zhao Xiaomin Yang Guanpin Yang Daxing Xu Guangtao Wang Wan Yang Pengjun Wang Zenglan Wang Shichao Xing |
| author_sort | Xiu Liu |
| collection | DOAJ |
| description | Some studies on the hyperuricemia (HUA) have focused on intestinal bacteria. To better understand the correlation between gut microbiota and HUA, we established a HUA rat model with high-purine diet, and used 16S rRNA genes sequencing to analyze gut microbiota changes in HUA rats. To analyze the potential role played by gut microbiota in HUA, we altered the gut microbiota of HUA rats with antibiotics, and compared the degree of uric acid elevation between HUA and antibiotic-fed HUA rats (Ab+HUA). Finally, we established a recipient rat model, in which we transplanted fecal microbiota of HUA and normal rats into recipient rats. Three weeks later, we compared the uric acid content of recipient rats. As a result, the diversity and abundance of the gut microbiota had changed in HUA rats. The Ab-fed HUA rats had significantly lower uric acid content compared to the HUA rats, and gut microbiota from HUA rats increased uric acid content of recipient rats. The genera Vallitalea, Christensenella and Insolitispirillum may associate with HUA. Our findings highlight the association between gut microbiota and HUA, and the potential role played by gut microbiota in HUA. We hope that this finding will promote the isolation and culture of HUA-related bacteria and orient HUA-related studies from being correlational to mechanistic. These steps will therefore make it possible for us to treat HUA using gut microbiota as the target. |
| first_indexed | 2024-03-09T06:44:09Z |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:44:09Z |
| publishDate | 2020-03-01 |
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| spelling | doaj.art-384175767fb34a87b84cbe5689fe42982023-12-03T10:40:56ZengPeerJ Inc.PeerJ2167-83592020-03-018e866410.7717/peerj.8664The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemiaXiu Liu0Qiulan Lv1Hongyan Ren2Liu Gao3Peng Zhao4Xiaomin Yang5Guanpin Yang6Daxing Xu7Guangtao Wang8Wan Yang9Pengjun Wang10Zenglan Wang11Shichao Xing12Department of Pathogenic Biology, School of Basic Medicine, Qingdao University, Qingdao, ChinaAffiliated Hospital of Qingdao University, Qingdao, ChinaShanghai Itechgene Technology Co.,Ltd, Shanghai, ChinaMedical Department of 3SBio Group, Shanghai, ChinaAffiliated Hospital of Qingdao University, Qingdao, ChinaAffiliated Hospital of Qingdao University, Qingdao, ChinaThe Key Laboratory of Mariculture of Ministry of Education, Ocean University of China, Qingdao, ChinaDepartment of Pathogenic Biology, School of Basic Medicine, Qingdao University, Qingdao, ChinaAffiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Pathogenic Biology, School of Basic Medicine, Qingdao University, Qingdao, ChinaAffiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Pathogenic Biology, School of Basic Medicine, Qingdao University, Qingdao, ChinaDepartment of Pathogenic Biology, School of Basic Medicine, Qingdao University, Qingdao, ChinaSome studies on the hyperuricemia (HUA) have focused on intestinal bacteria. To better understand the correlation between gut microbiota and HUA, we established a HUA rat model with high-purine diet, and used 16S rRNA genes sequencing to analyze gut microbiota changes in HUA rats. To analyze the potential role played by gut microbiota in HUA, we altered the gut microbiota of HUA rats with antibiotics, and compared the degree of uric acid elevation between HUA and antibiotic-fed HUA rats (Ab+HUA). Finally, we established a recipient rat model, in which we transplanted fecal microbiota of HUA and normal rats into recipient rats. Three weeks later, we compared the uric acid content of recipient rats. As a result, the diversity and abundance of the gut microbiota had changed in HUA rats. The Ab-fed HUA rats had significantly lower uric acid content compared to the HUA rats, and gut microbiota from HUA rats increased uric acid content of recipient rats. The genera Vallitalea, Christensenella and Insolitispirillum may associate with HUA. Our findings highlight the association between gut microbiota and HUA, and the potential role played by gut microbiota in HUA. We hope that this finding will promote the isolation and culture of HUA-related bacteria and orient HUA-related studies from being correlational to mechanistic. These steps will therefore make it possible for us to treat HUA using gut microbiota as the target.https://peerj.com/articles/8664.pdfGut microbiotaHyperuricemiaCorrelationChangeGut microbiota disorderFecal microbiota transplantation |
| spellingShingle | Xiu Liu Qiulan Lv Hongyan Ren Liu Gao Peng Zhao Xiaomin Yang Guanpin Yang Daxing Xu Guangtao Wang Wan Yang Pengjun Wang Zenglan Wang Shichao Xing The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia PeerJ Gut microbiota Hyperuricemia Correlation Change Gut microbiota disorder Fecal microbiota transplantation |
| title | The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia |
| title_full | The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia |
| title_fullStr | The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia |
| title_full_unstemmed | The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia |
| title_short | The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia |
| title_sort | altered gut microbiota of high purine induced hyperuricemia rats and its correlation with hyperuricemia |
| topic | Gut microbiota Hyperuricemia Correlation Change Gut microbiota disorder Fecal microbiota transplantation |
| url | https://peerj.com/articles/8664.pdf |
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