Reduction in Toxicity of Nano-Ag-Polyvinyl-pyrrolidone Using <i>Hydra</i> Proteins and Peptides during Zebrafish Embryogenesis
<i>Hydra magnipapillata</i> cells reduce the toxicity of silver nanomaterials to zebrafish (<i>Danio rerio</i>) embryos. In this study, we investigated whether <i>Hydra</i> protein (HP) and <i>Hydra</i> basal disc peptide (Hym176) materials reduce nano...
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| Format: | Article |
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MDPI AG
2019-08-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/9/9/1210 |
| _version_ | 1818982560481411072 |
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| author | Soon Seok Kim Jin Ah Lee Min-Kyeong Yeo |
| author_facet | Soon Seok Kim Jin Ah Lee Min-Kyeong Yeo |
| author_sort | Soon Seok Kim |
| collection | DOAJ |
| description | <i>Hydra magnipapillata</i> cells reduce the toxicity of silver nanomaterials to zebrafish (<i>Danio rerio</i>) embryos. In this study, we investigated whether <i>Hydra</i> protein (HP) and <i>Hydra</i> basal disc peptide (Hym176) materials reduce nano-Ag-polyvinylpyrrolidone (N-Ag-PVP) toxicity during embryogenesis of the nanosensitive organism zebrafish. Protein (HP) was extracted from <i>Hydra</i>, and peptide (Hym176) was extracted from the hydra basal disc, which is attractive to nanomaterials and related to the immune system. The experimental conditions were exposure to N-Ag-PVP, HP, N-Ag-PVP+HP, Hym176, or N-Ag-PVP+Hym176 during embryo development. N-Ag-PVP+HP group showed lower toxicity than N-Ag-PVP group. In addition, in the N-Ag-PVP+HP group formed aggregated nanomaterials (≥200 nm size) through electrostatic bonding. In the gene expression profile, HP group differed in gene expression profile compared the other experimental groups and it was no genetic toxicity. HP showed a tendency to reduce side effects and abnormal gene expression produced by N-Ag-PVP with no evidence of inherent toxicity. Considering the potential nanotoxicity effects of released nanomaterials on the ecosystem, the reduction of nanotoxicity observed with HP natural materials should be regarded with great interest in terms of the overall health of the ecosystem. |
| first_indexed | 2024-12-20T17:49:09Z |
| format | Article |
| id | doaj.art-384563de6c074cb38c083b4b6030dde6 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-20T17:49:09Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-384563de6c074cb38c083b4b6030dde62022-12-21T19:30:54ZengMDPI AGNanomaterials2079-49912019-08-0199121010.3390/nano9091210nano9091210Reduction in Toxicity of Nano-Ag-Polyvinyl-pyrrolidone Using <i>Hydra</i> Proteins and Peptides during Zebrafish EmbryogenesisSoon Seok Kim0Jin Ah Lee1Min-Kyeong Yeo2Department of Environmental Science and Engineering, College of Engineering, Kyung Hee University, 1732 Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do, Seoul 17104, KoreaDepartment of Environmental Science and Engineering, College of Engineering, Kyung Hee University, 1732 Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do, Seoul 17104, KoreaDepartment of Environmental Science and Engineering, College of Engineering, Kyung Hee University, 1732 Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do, Seoul 17104, Korea<i>Hydra magnipapillata</i> cells reduce the toxicity of silver nanomaterials to zebrafish (<i>Danio rerio</i>) embryos. In this study, we investigated whether <i>Hydra</i> protein (HP) and <i>Hydra</i> basal disc peptide (Hym176) materials reduce nano-Ag-polyvinylpyrrolidone (N-Ag-PVP) toxicity during embryogenesis of the nanosensitive organism zebrafish. Protein (HP) was extracted from <i>Hydra</i>, and peptide (Hym176) was extracted from the hydra basal disc, which is attractive to nanomaterials and related to the immune system. The experimental conditions were exposure to N-Ag-PVP, HP, N-Ag-PVP+HP, Hym176, or N-Ag-PVP+Hym176 during embryo development. N-Ag-PVP+HP group showed lower toxicity than N-Ag-PVP group. In addition, in the N-Ag-PVP+HP group formed aggregated nanomaterials (≥200 nm size) through electrostatic bonding. In the gene expression profile, HP group differed in gene expression profile compared the other experimental groups and it was no genetic toxicity. HP showed a tendency to reduce side effects and abnormal gene expression produced by N-Ag-PVP with no evidence of inherent toxicity. Considering the potential nanotoxicity effects of released nanomaterials on the ecosystem, the reduction of nanotoxicity observed with HP natural materials should be regarded with great interest in terms of the overall health of the ecosystem.https://www.mdpi.com/2079-4991/9/9/1210nanotoxicitynano-Ag-polyvinylpyrrolidone<i>Hydra</i> protein<i>Hydra</i> peptidereduced toxicitymicroarray |
| spellingShingle | Soon Seok Kim Jin Ah Lee Min-Kyeong Yeo Reduction in Toxicity of Nano-Ag-Polyvinyl-pyrrolidone Using <i>Hydra</i> Proteins and Peptides during Zebrafish Embryogenesis Nanomaterials nanotoxicity nano-Ag-polyvinylpyrrolidone <i>Hydra</i> protein <i>Hydra</i> peptide reduced toxicity microarray |
| title | Reduction in Toxicity of Nano-Ag-Polyvinyl-pyrrolidone Using <i>Hydra</i> Proteins and Peptides during Zebrafish Embryogenesis |
| title_full | Reduction in Toxicity of Nano-Ag-Polyvinyl-pyrrolidone Using <i>Hydra</i> Proteins and Peptides during Zebrafish Embryogenesis |
| title_fullStr | Reduction in Toxicity of Nano-Ag-Polyvinyl-pyrrolidone Using <i>Hydra</i> Proteins and Peptides during Zebrafish Embryogenesis |
| title_full_unstemmed | Reduction in Toxicity of Nano-Ag-Polyvinyl-pyrrolidone Using <i>Hydra</i> Proteins and Peptides during Zebrafish Embryogenesis |
| title_short | Reduction in Toxicity of Nano-Ag-Polyvinyl-pyrrolidone Using <i>Hydra</i> Proteins and Peptides during Zebrafish Embryogenesis |
| title_sort | reduction in toxicity of nano ag polyvinyl pyrrolidone using i hydra i proteins and peptides during zebrafish embryogenesis |
| topic | nanotoxicity nano-Ag-polyvinylpyrrolidone <i>Hydra</i> protein <i>Hydra</i> peptide reduced toxicity microarray |
| url | https://www.mdpi.com/2079-4991/9/9/1210 |
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