BSR and Full-Length Transcriptome Approaches Identified Candidate Genes for High Seed Ratio in <i>Camellia vietnamensis</i>
(1) Background: <i>C. vietnamensis</i> is very suitable for growth in the low hilly areas of southern subtropical regions. Under appropriate conditions, the oil yield of <i>C. vietnamensis</i> can reach 1125 kg/ha (the existing varieties can reach 750 kg/ha). Moreover, the fr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
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| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/45/1/22 |
| Summary: | (1) Background: <i>C. vietnamensis</i> is very suitable for growth in the low hilly areas of southern subtropical regions. Under appropriate conditions, the oil yield of <i>C. vietnamensis</i> can reach 1125 kg/ha (the existing varieties can reach 750 kg/ha). Moreover, the fruit of <i>C. vietnamensis</i> is large and the pericarp is thick (>5 cm). Therefore, a high seed ratio has become the main target economic trait for the breeding of <i>C. vietnamensis.</i> (2) Methods: A half-sibling population of <i>C. vietnamensis</i> plants with a combination of high and low seed ratios was constructed by crossing a <i>C. vietnamensis</i> female parent. Bulked segregant RNA analysis and full-length transcriptome sequencing were performed to determine the molecular mechanisms underlying a high seed ratio. (3) Results: Seed ratio is a complex quantitative trait with a normal distribution, which is significantly associated with four other traits of fruit (seed weight, seed number, fruit diameter, and pericarp thickness). Two candidate regions related to high seed ratio (HSR) were predicted. One spanned 140.8–148.4 Mb of chromosome 2 and was associated with 97 seed-yield-related candidate genes ranging in length from 278 to 16,628 bp. The other spanned 35.3–37.3 Mb on chromosome 15 and was associated with 38 genes ranging in length from 221 to 16,928 bp. Using the full-length transcript as a template, a total of 115 candidate transcripts were obtained, and 78 transcripts were predicted to be functionally annotated. The DEGs from two set pairs of cDNA sequencing bulks were enriched to cytochrome p450 CYP76F14 (KOG0156; GO:0055114, <i>HSR4</i>, <i>HSR7</i>), the gibberellin phytohormone pathway (GO:0016787, <i>HSR5</i>), the calcium signaling pathway (GO:0005509, <i>HSR6</i>), the polyubiquitin-PPAR signaling pathway (GO:0005515, <i>HSR2</i>, <i>HSR3</i>), and several main transcription factors (bZIP transcription factor, <i>HSR1</i>) in <i>C. vietnamensis</i>. |
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| ISSN: | 1467-3037 1467-3045 |