Genetic loss of norepinephrine does not alter adult hippocampal neurogenesis in dopamine beta-hydroxylase deficient mice
Norepinephrine (NE), and specific adrenoceptors, have been reported to influence distinct aspects of adult hippocampal neurogenesis, including latent stem cell activation, progenitor proliferation, and differentiation. These findings are predominantly based on the use of pharmacological approaches i...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | IBRO Neuroscience Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667242122000835 |
| _version_ | 1811293332017512448 |
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| author | Darshana Kapri Krishna C. Vadodaria Karen S. Rommelfanger Yvonne E. Ogbonmwan L. Cameron Liles Kimberly A. Fernandes-Thomas Sonali S. Salvi Basma F.A. Husain David Weinshenker Vidita A. Vaidya |
| author_facet | Darshana Kapri Krishna C. Vadodaria Karen S. Rommelfanger Yvonne E. Ogbonmwan L. Cameron Liles Kimberly A. Fernandes-Thomas Sonali S. Salvi Basma F.A. Husain David Weinshenker Vidita A. Vaidya |
| author_sort | Darshana Kapri |
| collection | DOAJ |
| description | Norepinephrine (NE), and specific adrenoceptors, have been reported to influence distinct aspects of adult hippocampal neurogenesis, including latent stem cell activation, progenitor proliferation, and differentiation. These findings are predominantly based on the use of pharmacological approaches in both in vitro and in vivo systems. Here, we sought to assess the consequences of genetic ablation of NE on adult hippocampal neurogenesis, by examining dopamine β hydroxylase knockout (Dbh -/-) mice, which lack NE from birth. We find that Dbh -/- mice exhibit no difference in adult hippocampal progenitor proliferation and survival. Further, the number of immature newborn neurons, labeled using stage-specific developmental markers within the hippocampal neurogenic niche, was also unaltered in Dbh -/- mice. In contrast, the noradrenergic neurotoxin DSP-4, which had previously been shown to reduce adult hippocampal neurogenesis in rats, also resulted in a decline in hippocampal progenitor proliferation in C57/Bl6N mice. These findings indicate that pharmacological lesioning of noradrenergic afferents in adulthood, but not the complete genetic loss of NE from birth, impairs adult hippocampal neurogenesis in mice. |
| first_indexed | 2024-04-13T04:59:48Z |
| format | Article |
| id | doaj.art-384b53d4283246fbbb35144e26820ae1 |
| institution | Directory Open Access Journal |
| issn | 2667-2421 |
| language | English |
| last_indexed | 2024-04-13T04:59:48Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | IBRO Neuroscience Reports |
| spelling | doaj.art-384b53d4283246fbbb35144e26820ae12022-12-22T03:01:23ZengElsevierIBRO Neuroscience Reports2667-24212022-12-0113420425Genetic loss of norepinephrine does not alter adult hippocampal neurogenesis in dopamine beta-hydroxylase deficient miceDarshana Kapri0Krishna C. Vadodaria1Karen S. Rommelfanger2Yvonne E. Ogbonmwan3L. Cameron Liles4Kimberly A. Fernandes-Thomas5Sonali S. Salvi6Basma F.A. Husain7David Weinshenker8Vidita A. Vaidya9Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400005, IndiaDepartment of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400005, IndiaDepartment of Human Genetics, Emory University, Atlanta, GA 30322, USADepartment of Human Genetics, Emory University, Atlanta, GA 30322, USADepartment of Human Genetics, Emory University, Atlanta, GA 30322, USADepartment of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400005, IndiaDepartment of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400005, IndiaDepartment of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400005, IndiaDepartment of Human Genetics, Emory University, Atlanta, GA 30322, USA; Correspondence to: Department of Human Genetics, Emory University School of Medicine, 615 Michael St., Whitehead 301, Atlanta, GA 30322, USA.Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400005, India; Correspondence to: Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400005, India.Norepinephrine (NE), and specific adrenoceptors, have been reported to influence distinct aspects of adult hippocampal neurogenesis, including latent stem cell activation, progenitor proliferation, and differentiation. These findings are predominantly based on the use of pharmacological approaches in both in vitro and in vivo systems. Here, we sought to assess the consequences of genetic ablation of NE on adult hippocampal neurogenesis, by examining dopamine β hydroxylase knockout (Dbh -/-) mice, which lack NE from birth. We find that Dbh -/- mice exhibit no difference in adult hippocampal progenitor proliferation and survival. Further, the number of immature newborn neurons, labeled using stage-specific developmental markers within the hippocampal neurogenic niche, was also unaltered in Dbh -/- mice. In contrast, the noradrenergic neurotoxin DSP-4, which had previously been shown to reduce adult hippocampal neurogenesis in rats, also resulted in a decline in hippocampal progenitor proliferation in C57/Bl6N mice. These findings indicate that pharmacological lesioning of noradrenergic afferents in adulthood, but not the complete genetic loss of NE from birth, impairs adult hippocampal neurogenesis in mice.http://www.sciencedirect.com/science/article/pii/S2667242122000835DSP-4Dbh knockout miceDoublecortinBrdUHippocampal progenitorProliferation |
| spellingShingle | Darshana Kapri Krishna C. Vadodaria Karen S. Rommelfanger Yvonne E. Ogbonmwan L. Cameron Liles Kimberly A. Fernandes-Thomas Sonali S. Salvi Basma F.A. Husain David Weinshenker Vidita A. Vaidya Genetic loss of norepinephrine does not alter adult hippocampal neurogenesis in dopamine beta-hydroxylase deficient mice IBRO Neuroscience Reports DSP-4 Dbh knockout mice Doublecortin BrdU Hippocampal progenitor Proliferation |
| title | Genetic loss of norepinephrine does not alter adult hippocampal neurogenesis in dopamine beta-hydroxylase deficient mice |
| title_full | Genetic loss of norepinephrine does not alter adult hippocampal neurogenesis in dopamine beta-hydroxylase deficient mice |
| title_fullStr | Genetic loss of norepinephrine does not alter adult hippocampal neurogenesis in dopamine beta-hydroxylase deficient mice |
| title_full_unstemmed | Genetic loss of norepinephrine does not alter adult hippocampal neurogenesis in dopamine beta-hydroxylase deficient mice |
| title_short | Genetic loss of norepinephrine does not alter adult hippocampal neurogenesis in dopamine beta-hydroxylase deficient mice |
| title_sort | genetic loss of norepinephrine does not alter adult hippocampal neurogenesis in dopamine beta hydroxylase deficient mice |
| topic | DSP-4 Dbh knockout mice Doublecortin BrdU Hippocampal progenitor Proliferation |
| url | http://www.sciencedirect.com/science/article/pii/S2667242122000835 |
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