The Anticancer Effect of a Novel Quinoline Derivative 91b1 through Downregulation of <i>Lumican</i>
Quinoline derivatives have been reported to possess a wide range of pharmaceutical activities. Our group previously synthesized a series of quinoline compounds, in which compound 91b1 showed a significant anticancer effect. The purpose of this study was to evaluate the anticancer activity of compoun...
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| author | Yuanyuan Zhou Zhongguo Zhou Dessy Chan Po yee Chung Yongqi Wang Albert Sun chi Chan Simon Law Kim hung Lam Johnny Cheuk On Tang |
| author_facet | Yuanyuan Zhou Zhongguo Zhou Dessy Chan Po yee Chung Yongqi Wang Albert Sun chi Chan Simon Law Kim hung Lam Johnny Cheuk On Tang |
| author_sort | Yuanyuan Zhou |
| collection | DOAJ |
| description | Quinoline derivatives have been reported to possess a wide range of pharmaceutical activities. Our group previously synthesized a series of quinoline compounds, in which compound 91b1 showed a significant anticancer effect. The purpose of this study was to evaluate the anticancer activity of compound 91b1 in vitro and in vivo, and screen out its regulated target. A series of cancer cell lines and nontumor cell lines were treated with compound 91b1 by MTS cytotoxicity assay and cell-cycle assay. In vivo anticancer activity was evaluated by a xenografted model on nude mice. Target prediction of 91b1 was assessed by microarray assay and confirmed by pancancer analysis. Relative expression of the target gene <i>Lumican</i> was measured by qRT-PCR. 91b1 significantly reduced tumor size in the nude mice xenograft model. <i>Lumican</i> was downregulated after 91b1 treatment. <i>Lumican</i> was proven to increase tumorigenesis in vivo, as well as cancer cell migration, invasion, and proliferation in vitro. The results of this study suggest that the anticancer activity of compound 91b1 probably works through downregulating the gene <i>Lumican.</i> |
| first_indexed | 2024-03-09T19:00:25Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T19:00:25Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-384bc1b3af204d0983562bdff5f5e6252023-11-24T05:03:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123211318110.3390/ijms232113181The Anticancer Effect of a Novel Quinoline Derivative 91b1 through Downregulation of <i>Lumican</i>Yuanyuan Zhou0Zhongguo Zhou1Dessy Chan2Po yee Chung3Yongqi Wang4Albert Sun chi Chan5Simon Law6Kim hung Lam7Johnny Cheuk On Tang8School of Biomedical Engineering, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4032, AustraliaState Key Laboratory of Chemical Biology and Drug Discovery, Lo Ka Chung Centre for Natural Anticancer Drug, Development, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, ChinaState Key Laboratory of Chemical Biology and Drug Discovery, Lo Ka Chung Centre for Natural Anticancer Drug, Development, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, ChinaDepartment of Biosystems Science and Eng, Eidgenössische Technische Hochschule (ETH) Zürich, 4058 Basel, SwitzerlandSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaDepartment of Surgery, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, ChinaState Key Laboratory of Chemical Biology and Drug Discovery, Lo Ka Chung Centre for Natural Anticancer Drug, Development, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, ChinaState Key Laboratory of Chemical Biology and Drug Discovery, Lo Ka Chung Centre for Natural Anticancer Drug, Development, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, ChinaQuinoline derivatives have been reported to possess a wide range of pharmaceutical activities. Our group previously synthesized a series of quinoline compounds, in which compound 91b1 showed a significant anticancer effect. The purpose of this study was to evaluate the anticancer activity of compound 91b1 in vitro and in vivo, and screen out its regulated target. A series of cancer cell lines and nontumor cell lines were treated with compound 91b1 by MTS cytotoxicity assay and cell-cycle assay. In vivo anticancer activity was evaluated by a xenografted model on nude mice. Target prediction of 91b1 was assessed by microarray assay and confirmed by pancancer analysis. Relative expression of the target gene <i>Lumican</i> was measured by qRT-PCR. 91b1 significantly reduced tumor size in the nude mice xenograft model. <i>Lumican</i> was downregulated after 91b1 treatment. <i>Lumican</i> was proven to increase tumorigenesis in vivo, as well as cancer cell migration, invasion, and proliferation in vitro. The results of this study suggest that the anticancer activity of compound 91b1 probably works through downregulating the gene <i>Lumican.</i>https://www.mdpi.com/1422-0067/23/21/13181quinolineanticancer<i>Lumican</i>microarray |
| spellingShingle | Yuanyuan Zhou Zhongguo Zhou Dessy Chan Po yee Chung Yongqi Wang Albert Sun chi Chan Simon Law Kim hung Lam Johnny Cheuk On Tang The Anticancer Effect of a Novel Quinoline Derivative 91b1 through Downregulation of <i>Lumican</i> International Journal of Molecular Sciences quinoline anticancer <i>Lumican</i> microarray |
| title | The Anticancer Effect of a Novel Quinoline Derivative 91b1 through Downregulation of <i>Lumican</i> |
| title_full | The Anticancer Effect of a Novel Quinoline Derivative 91b1 through Downregulation of <i>Lumican</i> |
| title_fullStr | The Anticancer Effect of a Novel Quinoline Derivative 91b1 through Downregulation of <i>Lumican</i> |
| title_full_unstemmed | The Anticancer Effect of a Novel Quinoline Derivative 91b1 through Downregulation of <i>Lumican</i> |
| title_short | The Anticancer Effect of a Novel Quinoline Derivative 91b1 through Downregulation of <i>Lumican</i> |
| title_sort | anticancer effect of a novel quinoline derivative 91b1 through downregulation of i lumican i |
| topic | quinoline anticancer <i>Lumican</i> microarray |
| url | https://www.mdpi.com/1422-0067/23/21/13181 |
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