Biocidal Effectiveness of Selected Disinfectants Solutions Based on Water and Ozonated Water against <i>Listeria monocytogenes</i> Strains
The aim of this study was to compare the biocidal effectiveness of disinfectants solutions prepared with ozonated and non-ozonated water against <i>Listeria monocytogenes</i>. Six <i>L. monocytogenes</i> strains were the research material (four isolates from food: meat (LMO-M...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/7/5/127 |
| _version_ | 1811197148575825920 |
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| author | Krzysztof Skowron Ewa Wałecka-Zacharska Katarzyna Grudlewska Agata Białucha Natalia Wiktorczyk Agata Bartkowska Maria Kowalska Stefan Kruszewski Eugenia Gospodarek-Komkowska |
| author_facet | Krzysztof Skowron Ewa Wałecka-Zacharska Katarzyna Grudlewska Agata Białucha Natalia Wiktorczyk Agata Bartkowska Maria Kowalska Stefan Kruszewski Eugenia Gospodarek-Komkowska |
| author_sort | Krzysztof Skowron |
| collection | DOAJ |
| description | The aim of this study was to compare the biocidal effectiveness of disinfectants solutions prepared with ozonated and non-ozonated water against <i>Listeria monocytogenes</i>. Six <i>L. monocytogenes</i> strains were the research material (four isolates from food: meat (LMO-M), dairy products (LMO-N), vegetables (LMO-W), and fish (LMO-R); one clinical strain (LMO-K) and reference strain ATCC 19111). The evaluation of the biocidal effectiveness of disinfectant solutions (QAC—quaternary ammonium compounds; OA—oxidizing agents; ChC—chlorine compounds; IC—iodine compounds; NANO—nanoparticles) was carried out, marking the MBC values. Based on the obtained results, the effectiveness coefficient (A) were calculated. The smaller the A value, the greater the efficiency of disinfection solutions prepared on the basis of ozonated versus non-ozonated water. Ozonated water showed biocidal efficacy against <i>L. monocytogenes</i>.<i> </i>Among tested disinfectentants, independent on type of water used for preparation, the most effective against <i>L. monocytogenes </i>were: QAC 1 (benzyl-C12-18-alkydimethyl ammonium chlorides) (1.00 × 10<sup>−</sup><sup>5</sup>–1.00 × 10<sup>−</sup><sup>4</sup> g/mL) in quaternary ammonium compounds, OA 3 (peracetic acid, hydrogen peroxide, bis (sulphate) bis (peroxymonosulfate)) (3.08 × 10<sup>−</sup><sup>4</sup> –3.70 × 10<sup>−</sup><sup>3</sup> g/mL) in oxidizing agents, ChC 1 (chlorine dioxide) (5.00 × 10<sup>−</sup><sup>8</sup> –7.00 × 10<sup>−</sup><sup>7</sup> g/mL) in chlorine compounds, IC 1 (iodine) (1.05–2.15 g/mL) in iodine compounds, and NANO 1 (nanocopper) (1.08 × 10<sup>-4 </sup>– 1.47 × 10<sup>−</sup><sup>4</sup> g/mL) in nanoparticles. The values of the activity coefficient for QAC ranged from 0.10 to 0.40, for OA—0.15–0.84, for ChC—0.25–0.83, for IC—0.45–0.60, and for NANO—0.70–0.84. The preparation of disinfectants solution on the basis of ozonated water, improved the microbicidal efficiency of the tested disinfectant, especially the quaternary ammonium compounds. An innovative element of our work is the use of ozonated water for the preparation of working solutions of the disinfection agents. Use ozonated water can help to reduce the use of disinfectant concentrations and limit the increasing of microbial resistance to disinfectants. This paper provides many new information to optimize hygiene plans in food processing plants and limit the spread of microorganisms such as L. monocytogenes. |
| first_indexed | 2024-04-12T01:09:43Z |
| format | Article |
| id | doaj.art-385310a787164cae9f0bf7aba8c55a1d |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-04-12T01:09:43Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-385310a787164cae9f0bf7aba8c55a1d2022-12-22T03:54:08ZengMDPI AGMicroorganisms2076-26072019-05-017512710.3390/microorganisms7050127microorganisms7050127Biocidal Effectiveness of Selected Disinfectants Solutions Based on Water and Ozonated Water against <i>Listeria monocytogenes</i> StrainsKrzysztof Skowron0Ewa Wałecka-Zacharska1Katarzyna Grudlewska2Agata Białucha3Natalia Wiktorczyk4Agata Bartkowska5Maria Kowalska6Stefan Kruszewski7Eugenia Gospodarek-Komkowska8Department of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Skłodowska-Curie St., 85-094 Bydgoszcz, PolandDepartment of Food Hygiene and Consumer Health, Wrocław University of Environmental and Life Sciences, 31 C.K. Norwida St., 50-375 Wrocław, PolandDepartment of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Skłodowska-Curie St., 85-094 Bydgoszcz, PolandDepartment of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Skłodowska-Curie St., 85-094 Bydgoszcz, PolandDepartment of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Skłodowska-Curie St., 85-094 Bydgoszcz, PolandDepartment of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Skłodowska-Curie St., 85-094 Bydgoszcz, PolandDepartment of Food Analytics and Environmental Protection, Faculty of Chemical Technology and Engineering, UTP University of Science and Technology, Seminaryjna 3, 85-326 Bydgoszcz, PolandBiophysics Department, Faculty of Pharmacy, Collegium Medicum of Nicolaus Copernicus University, Jagiellońska 13-15 St., 85–067 Bydgoszcz, PolandDepartment of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Skłodowska-Curie St., 85-094 Bydgoszcz, PolandThe aim of this study was to compare the biocidal effectiveness of disinfectants solutions prepared with ozonated and non-ozonated water against <i>Listeria monocytogenes</i>. Six <i>L. monocytogenes</i> strains were the research material (four isolates from food: meat (LMO-M), dairy products (LMO-N), vegetables (LMO-W), and fish (LMO-R); one clinical strain (LMO-K) and reference strain ATCC 19111). The evaluation of the biocidal effectiveness of disinfectant solutions (QAC—quaternary ammonium compounds; OA—oxidizing agents; ChC—chlorine compounds; IC—iodine compounds; NANO—nanoparticles) was carried out, marking the MBC values. Based on the obtained results, the effectiveness coefficient (A) were calculated. The smaller the A value, the greater the efficiency of disinfection solutions prepared on the basis of ozonated versus non-ozonated water. Ozonated water showed biocidal efficacy against <i>L. monocytogenes</i>.<i> </i>Among tested disinfectentants, independent on type of water used for preparation, the most effective against <i>L. monocytogenes </i>were: QAC 1 (benzyl-C12-18-alkydimethyl ammonium chlorides) (1.00 × 10<sup>−</sup><sup>5</sup>–1.00 × 10<sup>−</sup><sup>4</sup> g/mL) in quaternary ammonium compounds, OA 3 (peracetic acid, hydrogen peroxide, bis (sulphate) bis (peroxymonosulfate)) (3.08 × 10<sup>−</sup><sup>4</sup> –3.70 × 10<sup>−</sup><sup>3</sup> g/mL) in oxidizing agents, ChC 1 (chlorine dioxide) (5.00 × 10<sup>−</sup><sup>8</sup> –7.00 × 10<sup>−</sup><sup>7</sup> g/mL) in chlorine compounds, IC 1 (iodine) (1.05–2.15 g/mL) in iodine compounds, and NANO 1 (nanocopper) (1.08 × 10<sup>-4 </sup>– 1.47 × 10<sup>−</sup><sup>4</sup> g/mL) in nanoparticles. The values of the activity coefficient for QAC ranged from 0.10 to 0.40, for OA—0.15–0.84, for ChC—0.25–0.83, for IC—0.45–0.60, and for NANO—0.70–0.84. The preparation of disinfectants solution on the basis of ozonated water, improved the microbicidal efficiency of the tested disinfectant, especially the quaternary ammonium compounds. An innovative element of our work is the use of ozonated water for the preparation of working solutions of the disinfection agents. Use ozonated water can help to reduce the use of disinfectant concentrations and limit the increasing of microbial resistance to disinfectants. This paper provides many new information to optimize hygiene plans in food processing plants and limit the spread of microorganisms such as L. monocytogenes.https://www.mdpi.com/2076-2607/7/5/127<i>Listeria monocytogenes</i>ozonozonated waternon-ozonated waterdisinfectantsbiocidal effectiveness |
| spellingShingle | Krzysztof Skowron Ewa Wałecka-Zacharska Katarzyna Grudlewska Agata Białucha Natalia Wiktorczyk Agata Bartkowska Maria Kowalska Stefan Kruszewski Eugenia Gospodarek-Komkowska Biocidal Effectiveness of Selected Disinfectants Solutions Based on Water and Ozonated Water against <i>Listeria monocytogenes</i> Strains Microorganisms <i>Listeria monocytogenes</i> ozon ozonated water non-ozonated water disinfectants biocidal effectiveness |
| title | Biocidal Effectiveness of Selected Disinfectants Solutions Based on Water and Ozonated Water against <i>Listeria monocytogenes</i> Strains |
| title_full | Biocidal Effectiveness of Selected Disinfectants Solutions Based on Water and Ozonated Water against <i>Listeria monocytogenes</i> Strains |
| title_fullStr | Biocidal Effectiveness of Selected Disinfectants Solutions Based on Water and Ozonated Water against <i>Listeria monocytogenes</i> Strains |
| title_full_unstemmed | Biocidal Effectiveness of Selected Disinfectants Solutions Based on Water and Ozonated Water against <i>Listeria monocytogenes</i> Strains |
| title_short | Biocidal Effectiveness of Selected Disinfectants Solutions Based on Water and Ozonated Water against <i>Listeria monocytogenes</i> Strains |
| title_sort | biocidal effectiveness of selected disinfectants solutions based on water and ozonated water against i listeria monocytogenes i strains |
| topic | <i>Listeria monocytogenes</i> ozon ozonated water non-ozonated water disinfectants biocidal effectiveness |
| url | https://www.mdpi.com/2076-2607/7/5/127 |
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