Construction of Recombinant <i>Escherichia coli</i> with a High L-Phenylalanine Production Yield from Glucose
L-phenylalanine is an important aromatic amino acid that is widely used in the area of feed, food additives, and pharmaceuticals. Among the different strategies of L-phenylalanine synthesis, direct microbial fermentation from raw substrates has attracted more and more attention due to its environmen...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Microbiology Research |
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| Online Access: | https://www.mdpi.com/2036-7481/14/3/79 |
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| author | Pengfei Gu Shuo Zhao Chengwei Li Shuixing Jiang Hao Zhou Qiang Li |
| author_facet | Pengfei Gu Shuo Zhao Chengwei Li Shuixing Jiang Hao Zhou Qiang Li |
| author_sort | Pengfei Gu |
| collection | DOAJ |
| description | L-phenylalanine is an important aromatic amino acid that is widely used in the area of feed, food additives, and pharmaceuticals. Among the different strategies of L-phenylalanine synthesis, direct microbial fermentation from raw substrates has attracted more and more attention due to its environment friendly process and low-cost raw materials. In this study, a rational designed recombinant <i>Escherichia coli</i> was constructed for L-phenylalanine production. Based on wild type <i>E. coli</i> MG1655, multilevel engineering strategies were carried out, such as directing more carbon flux into the L-phenylalanine synthetic pathway, increasing intracellular level of precursors, blocking by-product synthesis pathways and facilitating the secretion of L-phenylalanine. During 5 L fed batch fermentation, recombinant <i>E. coli</i> MPH-3 could produce 19.24 g/L of L-phenylalanine with a yield of 0.279 g/g glucose. To the best of our knowledge, this is one of the highest yields of L-phenylalanine producing <i>E. coli</i> using glucose as the sole carbon source in fed-batch fermentation. |
| first_indexed | 2024-03-10T22:27:49Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2036-7481 |
| language | English |
| last_indexed | 2024-03-10T22:27:49Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
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| series | Microbiology Research |
| spelling | doaj.art-385a261c0b424f0796e612ddc2c9a6662023-11-19T11:58:24ZengMDPI AGMicrobiology Research2036-74812023-08-011431185119810.3390/microbiolres14030079Construction of Recombinant <i>Escherichia coli</i> with a High L-Phenylalanine Production Yield from GlucosePengfei Gu0Shuo Zhao1Chengwei Li2Shuixing Jiang3Hao Zhou4Qiang Li5School of Biological Science and Technology, University of Jinan, Jinan 250022, ChinaSchool of Biological Science and Technology, University of Jinan, Jinan 250022, ChinaRZBC Group Co., Ltd., Rizhao 276800, ChinaRZBC Group Co., Ltd., Rizhao 276800, ChinaRZBC Group Co., Ltd., Rizhao 276800, ChinaSchool of Biological Science and Technology, University of Jinan, Jinan 250022, ChinaL-phenylalanine is an important aromatic amino acid that is widely used in the area of feed, food additives, and pharmaceuticals. Among the different strategies of L-phenylalanine synthesis, direct microbial fermentation from raw substrates has attracted more and more attention due to its environment friendly process and low-cost raw materials. In this study, a rational designed recombinant <i>Escherichia coli</i> was constructed for L-phenylalanine production. Based on wild type <i>E. coli</i> MG1655, multilevel engineering strategies were carried out, such as directing more carbon flux into the L-phenylalanine synthetic pathway, increasing intracellular level of precursors, blocking by-product synthesis pathways and facilitating the secretion of L-phenylalanine. During 5 L fed batch fermentation, recombinant <i>E. coli</i> MPH-3 could produce 19.24 g/L of L-phenylalanine with a yield of 0.279 g/g glucose. To the best of our knowledge, this is one of the highest yields of L-phenylalanine producing <i>E. coli</i> using glucose as the sole carbon source in fed-batch fermentation.https://www.mdpi.com/2036-7481/14/3/79<i>E. coli</i>L-phenylalaninemetabolic engineeringglucose utilizationGlfyield |
| spellingShingle | Pengfei Gu Shuo Zhao Chengwei Li Shuixing Jiang Hao Zhou Qiang Li Construction of Recombinant <i>Escherichia coli</i> with a High L-Phenylalanine Production Yield from Glucose Microbiology Research <i>E. coli</i> L-phenylalanine metabolic engineering glucose utilization Glf yield |
| title | Construction of Recombinant <i>Escherichia coli</i> with a High L-Phenylalanine Production Yield from Glucose |
| title_full | Construction of Recombinant <i>Escherichia coli</i> with a High L-Phenylalanine Production Yield from Glucose |
| title_fullStr | Construction of Recombinant <i>Escherichia coli</i> with a High L-Phenylalanine Production Yield from Glucose |
| title_full_unstemmed | Construction of Recombinant <i>Escherichia coli</i> with a High L-Phenylalanine Production Yield from Glucose |
| title_short | Construction of Recombinant <i>Escherichia coli</i> with a High L-Phenylalanine Production Yield from Glucose |
| title_sort | construction of recombinant i escherichia coli i with a high l phenylalanine production yield from glucose |
| topic | <i>E. coli</i> L-phenylalanine metabolic engineering glucose utilization Glf yield |
| url | https://www.mdpi.com/2036-7481/14/3/79 |
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