Overexpression of Heat Shock Cognate Protein 71 kDa and Pyruvate Dehydrogenase in the Brain Tissue at the Early Stage of High Fat Diet Consumption
High-fat diet (HFD) increases the risk of obese, while obesity increases the prevalence of metabolic syndrome and non-communicable diseases. Therefore, it will be interesting to evaluate the changes in metabolic parameters and brain profile upon the early consumption of HFD. In this study, a total...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2022-04-01
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| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/37740 |
| _version_ | 1811288661824634880 |
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| author | Yan-Fen Lee Xuan-Yi Sim Mohd Nazri Ismail Lay-Harn Gam |
| author_facet | Yan-Fen Lee Xuan-Yi Sim Mohd Nazri Ismail Lay-Harn Gam |
| author_sort | Yan-Fen Lee |
| collection | DOAJ |
| description |
High-fat diet (HFD) increases the risk of obese, while obesity increases the prevalence of metabolic syndrome and non-communicable diseases. Therefore, it will be interesting to evaluate the changes in metabolic parameters and brain profile upon the early consumption of HFD. In this study, a total of 12 Sprague-Dawley male rats were divided into two groups (n = 6), each group was fed with normal diet and HFD (39% of total calories from fats), respectively, for 6 weeks consecutively. The body weight, blood glucose, cholesterol and triglyceride values were measured. Furthermore, the brain and visceral adipose tissues were harvested at the end of the experiment. Protein was extracted from the brain tissue, and the protein extracts were separated by using two-dimensional gel electrophoresis and analyzed by liquid chromatography tandem mass spectrometric analysis (LC/MS/MS). In terms of food calorie, the rats fed with HFD consumed more energy than the rats fed with normal diet. Nevertheless, the blood triglyceride and cholesterol, and the visceral adipose tissue of both the HFD and normal diet fed rats were indifferent. At the molecular level, overexpression of stress proteins, namely heat shock cognate protein 71 kDa (Hsc70) and pyruvate dehydrogenase were detected in brain tissue of HFD group. These results suggest that HFD intake causing significant change in brain proteins profile at the early phase of its consumption when no clear metabolic changes were observed. This showed that the brain was affected by HFD.
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| first_indexed | 2024-04-13T03:41:09Z |
| format | Article |
| id | doaj.art-385a8f5ae60a4572977cb9edf69cb15c |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-04-13T03:41:09Z |
| publishDate | 2022-04-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-385a8f5ae60a4572977cb9edf69cb15c2022-12-22T03:04:09ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942022-04-0129410.4308/hjb.29.4.499-506Overexpression of Heat Shock Cognate Protein 71 kDa and Pyruvate Dehydrogenase in the Brain Tissue at the Early Stage of High Fat Diet ConsumptionYan-Fen Lee0Xuan-Yi Sim1Mohd Nazri Ismail2Lay-Harn Gam31USM-RIKEN International Centre of Aging Science, USM, Minden, Penang, Malaysia. 2School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Penang, Malaysia1USM-RIKEN International Centre of Aging Science, USM, Minden, Penang, Malaysia. 2School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Penang, MalaysiaAnalytical Biochemistry Research Centre (ABrC), USM, Minden, Penang, Malaysia1USM-RIKEN International Centre of Aging Science, USM, Minden, Penang, Malaysia. 2School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Penang, Malaysia High-fat diet (HFD) increases the risk of obese, while obesity increases the prevalence of metabolic syndrome and non-communicable diseases. Therefore, it will be interesting to evaluate the changes in metabolic parameters and brain profile upon the early consumption of HFD. In this study, a total of 12 Sprague-Dawley male rats were divided into two groups (n = 6), each group was fed with normal diet and HFD (39% of total calories from fats), respectively, for 6 weeks consecutively. The body weight, blood glucose, cholesterol and triglyceride values were measured. Furthermore, the brain and visceral adipose tissues were harvested at the end of the experiment. Protein was extracted from the brain tissue, and the protein extracts were separated by using two-dimensional gel electrophoresis and analyzed by liquid chromatography tandem mass spectrometric analysis (LC/MS/MS). In terms of food calorie, the rats fed with HFD consumed more energy than the rats fed with normal diet. Nevertheless, the blood triglyceride and cholesterol, and the visceral adipose tissue of both the HFD and normal diet fed rats were indifferent. At the molecular level, overexpression of stress proteins, namely heat shock cognate protein 71 kDa (Hsc70) and pyruvate dehydrogenase were detected in brain tissue of HFD group. These results suggest that HFD intake causing significant change in brain proteins profile at the early phase of its consumption when no clear metabolic changes were observed. This showed that the brain was affected by HFD. https://journal.ipb.ac.id/index.php/hayati/article/view/37740 |
| spellingShingle | Yan-Fen Lee Xuan-Yi Sim Mohd Nazri Ismail Lay-Harn Gam Overexpression of Heat Shock Cognate Protein 71 kDa and Pyruvate Dehydrogenase in the Brain Tissue at the Early Stage of High Fat Diet Consumption Hayati Journal of Biosciences |
| title | Overexpression of Heat Shock Cognate Protein 71 kDa and Pyruvate Dehydrogenase in the Brain Tissue at the Early Stage of High Fat Diet Consumption |
| title_full | Overexpression of Heat Shock Cognate Protein 71 kDa and Pyruvate Dehydrogenase in the Brain Tissue at the Early Stage of High Fat Diet Consumption |
| title_fullStr | Overexpression of Heat Shock Cognate Protein 71 kDa and Pyruvate Dehydrogenase in the Brain Tissue at the Early Stage of High Fat Diet Consumption |
| title_full_unstemmed | Overexpression of Heat Shock Cognate Protein 71 kDa and Pyruvate Dehydrogenase in the Brain Tissue at the Early Stage of High Fat Diet Consumption |
| title_short | Overexpression of Heat Shock Cognate Protein 71 kDa and Pyruvate Dehydrogenase in the Brain Tissue at the Early Stage of High Fat Diet Consumption |
| title_sort | overexpression of heat shock cognate protein 71 kda and pyruvate dehydrogenase in the brain tissue at the early stage of high fat diet consumption |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/37740 |
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