Immunohistochemical Expression (IE) of Oestrogen Receptors in the Intestines of Prepubertal Gilts Exposed to Zearalenone
This study was conducted to determine if a low monotonic dose of zearalenone (ZEN) affects the immunohistochemical expression (IE) of oestrogen receptor alpha (ERα) and oestrogen receptor beta (ERβ) in the intestines of sexually immature gilts. Group C (control group; n = 18) gilts were given a plac...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Toxins |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6651/15/2/122 |
| _version_ | 1797617975709138944 |
|---|---|
| author | Magdalena Gajęcka Iwona Otrocka-Domagała Paweł Brzuzan Michał Dąbrowski Sylwia Lisieska-Żołnierczyk Łukasz Zielonka Maciej Tadeusz Gajęcki |
| author_facet | Magdalena Gajęcka Iwona Otrocka-Domagała Paweł Brzuzan Michał Dąbrowski Sylwia Lisieska-Żołnierczyk Łukasz Zielonka Maciej Tadeusz Gajęcki |
| author_sort | Magdalena Gajęcka |
| collection | DOAJ |
| description | This study was conducted to determine if a low monotonic dose of zearalenone (ZEN) affects the immunohistochemical expression (IE) of oestrogen receptor alpha (ERα) and oestrogen receptor beta (ERβ) in the intestines of sexually immature gilts. Group C (control group; n = 18) gilts were given a placebo. Group E (experimental group; n = 18) gilts were dosed orally with 40 μg ZEN /kg body weight (BW), each day before morning feeding. Samples of intestinal tissue were collected post-mortem six times. The samples were stained to analyse the IE of ERα and Erβ in the scanned slides. The strongest response was observed in ERα in the duodenum (90.387—average % of cells with ERα expression) and in ERβ in the descending colon (84.329—average % of cells with ERβ expression); the opposite response was recorded in the caecum (2.484—average % of cells with ERα expression) and the ascending colon (2.448—average % of cells with ERα expression); on the first two dates of exposure, the digestive tract had to adapt to ZEN in feed. The results of this study, supported by a mechanistic interpretation of previous research findings, suggest that ZEN performs numerous functions in the digestive tract. |
| first_indexed | 2024-03-11T08:02:38Z |
| format | Article |
| id | doaj.art-385aff22ecaf44f2b6cb2452b17ac575 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-11T08:02:38Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-385aff22ecaf44f2b6cb2452b17ac5752023-11-16T23:38:51ZengMDPI AGToxins2072-66512023-02-0115212210.3390/toxins15020122Immunohistochemical Expression (IE) of Oestrogen Receptors in the Intestines of Prepubertal Gilts Exposed to ZearalenoneMagdalena Gajęcka0Iwona Otrocka-Domagała1Paweł Brzuzan2Michał Dąbrowski3Sylwia Lisieska-Żołnierczyk4Łukasz Zielonka5Maciej Tadeusz Gajęcki6Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, PolandDepartment of Pathological Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, PolandDepartment of Environmental Biotechnology, Faculty of Environmental Sciences and Fisheries, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, PolandDepartment of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, PolandIndependent Public Health Care Centre of the Ministry of the Interior and Administration, and the Warmia and Mazury Oncology Centre in Olsztyn, al. Wojska Polskiego 37, 10-228 Olsztyn, PolandDepartment of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, PolandDepartment of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, PolandThis study was conducted to determine if a low monotonic dose of zearalenone (ZEN) affects the immunohistochemical expression (IE) of oestrogen receptor alpha (ERα) and oestrogen receptor beta (ERβ) in the intestines of sexually immature gilts. Group C (control group; n = 18) gilts were given a placebo. Group E (experimental group; n = 18) gilts were dosed orally with 40 μg ZEN /kg body weight (BW), each day before morning feeding. Samples of intestinal tissue were collected post-mortem six times. The samples were stained to analyse the IE of ERα and Erβ in the scanned slides. The strongest response was observed in ERα in the duodenum (90.387—average % of cells with ERα expression) and in ERβ in the descending colon (84.329—average % of cells with ERβ expression); the opposite response was recorded in the caecum (2.484—average % of cells with ERα expression) and the ascending colon (2.448—average % of cells with ERα expression); on the first two dates of exposure, the digestive tract had to adapt to ZEN in feed. The results of this study, supported by a mechanistic interpretation of previous research findings, suggest that ZEN performs numerous functions in the digestive tract.https://www.mdpi.com/2072-6651/15/2/122zearalenoneimmunohistochemistryoestrogen receptorsgilts before puberty |
| spellingShingle | Magdalena Gajęcka Iwona Otrocka-Domagała Paweł Brzuzan Michał Dąbrowski Sylwia Lisieska-Żołnierczyk Łukasz Zielonka Maciej Tadeusz Gajęcki Immunohistochemical Expression (IE) of Oestrogen Receptors in the Intestines of Prepubertal Gilts Exposed to Zearalenone Toxins zearalenone immunohistochemistry oestrogen receptors gilts before puberty |
| title | Immunohistochemical Expression (IE) of Oestrogen Receptors in the Intestines of Prepubertal Gilts Exposed to Zearalenone |
| title_full | Immunohistochemical Expression (IE) of Oestrogen Receptors in the Intestines of Prepubertal Gilts Exposed to Zearalenone |
| title_fullStr | Immunohistochemical Expression (IE) of Oestrogen Receptors in the Intestines of Prepubertal Gilts Exposed to Zearalenone |
| title_full_unstemmed | Immunohistochemical Expression (IE) of Oestrogen Receptors in the Intestines of Prepubertal Gilts Exposed to Zearalenone |
| title_short | Immunohistochemical Expression (IE) of Oestrogen Receptors in the Intestines of Prepubertal Gilts Exposed to Zearalenone |
| title_sort | immunohistochemical expression ie of oestrogen receptors in the intestines of prepubertal gilts exposed to zearalenone |
| topic | zearalenone immunohistochemistry oestrogen receptors gilts before puberty |
| url | https://www.mdpi.com/2072-6651/15/2/122 |
| work_keys_str_mv | AT magdalenagajecka immunohistochemicalexpressionieofoestrogenreceptorsintheintestinesofprepubertalgiltsexposedtozearalenone AT iwonaotrockadomagała immunohistochemicalexpressionieofoestrogenreceptorsintheintestinesofprepubertalgiltsexposedtozearalenone AT pawełbrzuzan immunohistochemicalexpressionieofoestrogenreceptorsintheintestinesofprepubertalgiltsexposedtozearalenone AT michałdabrowski immunohistochemicalexpressionieofoestrogenreceptorsintheintestinesofprepubertalgiltsexposedtozearalenone AT sylwialisieskazołnierczyk immunohistochemicalexpressionieofoestrogenreceptorsintheintestinesofprepubertalgiltsexposedtozearalenone AT łukaszzielonka immunohistochemicalexpressionieofoestrogenreceptorsintheintestinesofprepubertalgiltsexposedtozearalenone AT maciejtadeuszgajecki immunohistochemicalexpressionieofoestrogenreceptorsintheintestinesofprepubertalgiltsexposedtozearalenone |