<em>Melanaphis</em> <em>sorghi</em> (Hemiptera: Aphididae) Clonal Diversity in the United States and Brazil
<i>Melanaphis sorghi</i> (Hemiptera: Aphididae), are an economically important pest to sorghum in the Americas. Previous studies have found that a super-clone that belongs to multilocus lineage (MLL)-F predominated in the U.S. from 2013 to 2018 and uses multiple hosts besides sorghum. In...
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MDPI AG
2022-04-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/5/416 |
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| author | Karen Harris-Shultz John Scott Armstrong Geraldo Carvalho Jurandir Pereira Segundo Xinzhi Ni |
| author_facet | Karen Harris-Shultz John Scott Armstrong Geraldo Carvalho Jurandir Pereira Segundo Xinzhi Ni |
| author_sort | Karen Harris-Shultz |
| collection | DOAJ |
| description | <i>Melanaphis sorghi</i> (Hemiptera: Aphididae), are an economically important pest to sorghum in the Americas. Previous studies have found that a super-clone that belongs to multilocus lineage (MLL)-F predominated in the U.S. from 2013 to 2018 and uses multiple hosts besides sorghum. In contrast, previous studies found that aphids in South America belong to MLL-C, but these studies only examined aphids collected from sugarcane. In this study we sought to determine if the superclone persisted in the U.S. in 2019–2020 and to determine the MLL of aphids found on sorghum in the largest country in South America, Brazil. <i>Melanaphis</i> spp. samples (121) were collected from the U.S. in 2019–2020 and Brazil in 2020 and were genotyped with 8–9 <i>Melanaphis</i> spp. microsatellite markers. Genotyping results showed that all samples from the U.S. in 2019 and Brazil in 2020 had alleles identical to the predominant superclone. Of the 52 samples collected in the U.S. in 2020, 50 samples were identical to the predominant super-clone (multilocus lineage-F; <i>M. sorghi</i>), while two samples from Texas differed from the super-clone by a single allele. The results demonstrated that the super-clone remains in the U.S. on sorghum, Johnsongrass, and giant miscanthus and is also present on sorghum within Brazil. |
| first_indexed | 2024-03-10T03:41:18Z |
| format | Article |
| id | doaj.art-385bbe019865480d97ea1fc3673c31f1 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T03:41:18Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-385bbe019865480d97ea1fc3673c31f12023-11-23T11:31:16ZengMDPI AGInsects2075-44502022-04-0113541610.3390/insects13050416<em>Melanaphis</em> <em>sorghi</em> (Hemiptera: Aphididae) Clonal Diversity in the United States and BrazilKaren Harris-Shultz0John Scott Armstrong1Geraldo Carvalho2Jurandir Pereira Segundo3Xinzhi Ni4Crop Genetics and Breeding Research Unit, USDA-ARS, 115 Coastal Way, Tifton, GA 31793, USAWheat, Peanut and Other Field Crops Research Unit, USDA-ARS, 1301 N. Western Rd, Stillwater, OK 74075, USAHelix Sementes e Mudas LTDA, Rodovia MT-242, KM 19, Zona Rural, Sorriso 78890-000, BrazilHelix Sementes e Mudas LTDA, Rua Arnaldo Luiz de Oliveira, 75 Bela Vista, Patos de Minas 38703-240, BrazilCrop Genetics and Breeding Research Unit, USDA-ARS, 115 Coastal Way, Tifton, GA 31793, USA<i>Melanaphis sorghi</i> (Hemiptera: Aphididae), are an economically important pest to sorghum in the Americas. Previous studies have found that a super-clone that belongs to multilocus lineage (MLL)-F predominated in the U.S. from 2013 to 2018 and uses multiple hosts besides sorghum. In contrast, previous studies found that aphids in South America belong to MLL-C, but these studies only examined aphids collected from sugarcane. In this study we sought to determine if the superclone persisted in the U.S. in 2019–2020 and to determine the MLL of aphids found on sorghum in the largest country in South America, Brazil. <i>Melanaphis</i> spp. samples (121) were collected from the U.S. in 2019–2020 and Brazil in 2020 and were genotyped with 8–9 <i>Melanaphis</i> spp. microsatellite markers. Genotyping results showed that all samples from the U.S. in 2019 and Brazil in 2020 had alleles identical to the predominant superclone. Of the 52 samples collected in the U.S. in 2020, 50 samples were identical to the predominant super-clone (multilocus lineage-F; <i>M. sorghi</i>), while two samples from Texas differed from the super-clone by a single allele. The results demonstrated that the super-clone remains in the U.S. on sorghum, Johnsongrass, and giant miscanthus and is also present on sorghum within Brazil.https://www.mdpi.com/2075-4450/13/5/416super-clone<i>Melanaphis sacchari</i>microsatellitesimple sequence repeatsorghum aphidmultilocus lineage |
| spellingShingle | Karen Harris-Shultz John Scott Armstrong Geraldo Carvalho Jurandir Pereira Segundo Xinzhi Ni <em>Melanaphis</em> <em>sorghi</em> (Hemiptera: Aphididae) Clonal Diversity in the United States and Brazil Insects super-clone <i>Melanaphis sacchari</i> microsatellite simple sequence repeat sorghum aphid multilocus lineage |
| title | <em>Melanaphis</em> <em>sorghi</em> (Hemiptera: Aphididae) Clonal Diversity in the United States and Brazil |
| title_full | <em>Melanaphis</em> <em>sorghi</em> (Hemiptera: Aphididae) Clonal Diversity in the United States and Brazil |
| title_fullStr | <em>Melanaphis</em> <em>sorghi</em> (Hemiptera: Aphididae) Clonal Diversity in the United States and Brazil |
| title_full_unstemmed | <em>Melanaphis</em> <em>sorghi</em> (Hemiptera: Aphididae) Clonal Diversity in the United States and Brazil |
| title_short | <em>Melanaphis</em> <em>sorghi</em> (Hemiptera: Aphididae) Clonal Diversity in the United States and Brazil |
| title_sort | em melanaphis em em sorghi em hemiptera aphididae clonal diversity in the united states and brazil |
| topic | super-clone <i>Melanaphis sacchari</i> microsatellite simple sequence repeat sorghum aphid multilocus lineage |
| url | https://www.mdpi.com/2075-4450/13/5/416 |
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