Validation of the anti-infective potential of a polyherbal ‘Panchvalkal’ preparation, and elucidation of the molecular basis underlining its efficacy against Pseudomonas aeruginosa
Abstract Background A Panchvalkal formulation (Pentaphyte P-5®) mentioned in ancient texts of Indian traditional medicine was investigated for its anti-infective potential against Pseudomonas aeruginosa. Methods Effect of the test formulation on bacterial growth and pigment production was evaluated...
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| Format: | Article |
| Language: | English |
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BMC
2019-01-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-019-2428-5 |
| _version_ | 1819085168814587904 |
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| author | Chinmayi Joshi Pooja Patel Hanmanthrao Palep Vijay Kothari |
| author_facet | Chinmayi Joshi Pooja Patel Hanmanthrao Palep Vijay Kothari |
| author_sort | Chinmayi Joshi |
| collection | DOAJ |
| description | Abstract Background A Panchvalkal formulation (Pentaphyte P-5®) mentioned in ancient texts of Indian traditional medicine was investigated for its anti-infective potential against Pseudomonas aeruginosa. Methods Effect of the test formulation on bacterial growth and pigment production was evaluated by broth dilution assay. In vivo efficacy was evaluated using Caenorhabditis elegans as the model host. Whole transcriptome approach was taken to study the effect of test formulation on bacterial gene expression. Results This formulation in vitro was found to be capable of affecting quorum sensing (QS)-regulated traits (pyocyanin, pyoverdine, biofilm) of Pseudomonas aeruginosa. In combination with antibiotics, it enhanced susceptibility of the test bacterium to antibiotics like cephalexin and tetracycline. Effect of Panchvalkal formulation (PF) on QS-regulated traits of P. aeruginosa was not reversed even after repeated exposure of the bacterium to PF. In vivo efficacy of PF was demonstrated employing Caenorhabditis elegans as the model host, wherein PF-treated bacteria were able to kill lesser worms than their extract-unexposed counterparts. Whole transcriptome study revealed that approximately 14% of the P. aeruginosa genome was expressed differently under the influence of PF. Conclusions Major mechanisms through which Panchvalkal seems to exert its anti-virulence effect are generation of nitrosative and oxidative stress, and disturbing iron and molybdenum homeostasis, besides interfering with QS machinery. This study is a good demonstration of the therapeutic utility of the ‘polyherbalism’ concept, so common in ayurved. It also demonstrates utility of the modern ‘omics’ tools for validating the traditional medicine i.e. ayuromics. |
| first_indexed | 2024-12-21T21:00:04Z |
| format | Article |
| id | doaj.art-385ec675c72c4f52bf4803359460df07 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-21T21:00:04Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-385ec675c72c4f52bf4803359460df072022-12-21T18:50:28ZengBMCBMC Complementary and Alternative Medicine1472-68822019-01-0119111510.1186/s12906-019-2428-5Validation of the anti-infective potential of a polyherbal ‘Panchvalkal’ preparation, and elucidation of the molecular basis underlining its efficacy against Pseudomonas aeruginosaChinmayi Joshi0Pooja Patel1Hanmanthrao Palep2Vijay Kothari3Institute of Science, Nirma UniversityInstitute of Science, Nirma UniversityDr. Palep’s Medical Research FoundationInstitute of Science, Nirma UniversityAbstract Background A Panchvalkal formulation (Pentaphyte P-5®) mentioned in ancient texts of Indian traditional medicine was investigated for its anti-infective potential against Pseudomonas aeruginosa. Methods Effect of the test formulation on bacterial growth and pigment production was evaluated by broth dilution assay. In vivo efficacy was evaluated using Caenorhabditis elegans as the model host. Whole transcriptome approach was taken to study the effect of test formulation on bacterial gene expression. Results This formulation in vitro was found to be capable of affecting quorum sensing (QS)-regulated traits (pyocyanin, pyoverdine, biofilm) of Pseudomonas aeruginosa. In combination with antibiotics, it enhanced susceptibility of the test bacterium to antibiotics like cephalexin and tetracycline. Effect of Panchvalkal formulation (PF) on QS-regulated traits of P. aeruginosa was not reversed even after repeated exposure of the bacterium to PF. In vivo efficacy of PF was demonstrated employing Caenorhabditis elegans as the model host, wherein PF-treated bacteria were able to kill lesser worms than their extract-unexposed counterparts. Whole transcriptome study revealed that approximately 14% of the P. aeruginosa genome was expressed differently under the influence of PF. Conclusions Major mechanisms through which Panchvalkal seems to exert its anti-virulence effect are generation of nitrosative and oxidative stress, and disturbing iron and molybdenum homeostasis, besides interfering with QS machinery. This study is a good demonstration of the therapeutic utility of the ‘polyherbalism’ concept, so common in ayurved. It also demonstrates utility of the modern ‘omics’ tools for validating the traditional medicine i.e. ayuromics.http://link.springer.com/article/10.1186/s12906-019-2428-5Quorum sensingPanchvalkalPolyherbalPseudomonas aeruginosaTranscriptomeAyurved |
| spellingShingle | Chinmayi Joshi Pooja Patel Hanmanthrao Palep Vijay Kothari Validation of the anti-infective potential of a polyherbal ‘Panchvalkal’ preparation, and elucidation of the molecular basis underlining its efficacy against Pseudomonas aeruginosa BMC Complementary and Alternative Medicine Quorum sensing Panchvalkal Polyherbal Pseudomonas aeruginosa Transcriptome Ayurved |
| title | Validation of the anti-infective potential of a polyherbal ‘Panchvalkal’ preparation, and elucidation of the molecular basis underlining its efficacy against Pseudomonas aeruginosa |
| title_full | Validation of the anti-infective potential of a polyherbal ‘Panchvalkal’ preparation, and elucidation of the molecular basis underlining its efficacy against Pseudomonas aeruginosa |
| title_fullStr | Validation of the anti-infective potential of a polyherbal ‘Panchvalkal’ preparation, and elucidation of the molecular basis underlining its efficacy against Pseudomonas aeruginosa |
| title_full_unstemmed | Validation of the anti-infective potential of a polyherbal ‘Panchvalkal’ preparation, and elucidation of the molecular basis underlining its efficacy against Pseudomonas aeruginosa |
| title_short | Validation of the anti-infective potential of a polyherbal ‘Panchvalkal’ preparation, and elucidation of the molecular basis underlining its efficacy against Pseudomonas aeruginosa |
| title_sort | validation of the anti infective potential of a polyherbal panchvalkal preparation and elucidation of the molecular basis underlining its efficacy against pseudomonas aeruginosa |
| topic | Quorum sensing Panchvalkal Polyherbal Pseudomonas aeruginosa Transcriptome Ayurved |
| url | http://link.springer.com/article/10.1186/s12906-019-2428-5 |
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