Laboratory study: evaluation and comparison of physicochemical properties, level of surface charge and cytotoxicity of lipid nanocomposites for carrying nucleic acid in bone cancer
Introdution: The aim of this study was to investigate the physico-chemical properties, surface charge and cytotoxicity of nanocomposite formulations for the design of lipid nano-systems with maximum loading for nucleic acids in bone marrow cancer. Methods For this study, six types of liposomes with...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | fas |
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Shahid Sadoughi University of Medical Sciences
2018-06-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| Subjects: | |
| Online Access: | http://jssu.ssu.ac.ir/browse.php?a_code=A-10-303-4&slc_lang=en&sid=1 |
| _version_ | 1818358682073694208 |
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| author | Fatemeh Barzegari firouzabadi Shahrbanoo Oryan Mohammad Hasan Sheikhha Seyed Mehdi Kalantar Ameneh javed |
| author_facet | Fatemeh Barzegari firouzabadi Shahrbanoo Oryan Mohammad Hasan Sheikhha Seyed Mehdi Kalantar Ameneh javed |
| author_sort | Fatemeh Barzegari firouzabadi |
| collection | DOAJ |
| description | Introdution: The aim of this study was to investigate the physico-chemical properties, surface charge and cytotoxicity of nanocomposite formulations for the design of lipid nano-systems with maximum loading for nucleic acids in bone marrow cancer.
Methods For this study, six types of liposomes with different compositions were synthesized using DPPC, cholesterol, DSPE-mPEG (2000) and DOTAP. Then, nanosystems were evaluated for particle size, surface charge, cytotoxicity and loading rate of miRNA in two MG-63 and SaOs2 cell lines, which are human cell bone marrow cell lines.
Results: All nano-systems were monodisperse. Among the systems, the F6 formula has the lowest toxicity and the highest load of miRNA-143, due to the presence of a suitable amount of DOTAP and PEG phospholipid in the liposome structure.
Conclusion: Liposomal formulation with a suitable percentage of DOTAP cationic phospholipid can be used as a successful carrier for the transport of miRNA in the gene therapy process for the treatment of various cancers, especially metastatic types. |
| first_indexed | 2024-12-13T20:32:53Z |
| format | Article |
| id | doaj.art-385f5e9e2be44328902c421efb785437 |
| institution | Directory Open Access Journal |
| issn | 2228-5741 2228-5733 |
| language | fas |
| last_indexed | 2024-12-13T20:32:53Z |
| publishDate | 2018-06-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| spelling | doaj.art-385f5e9e2be44328902c421efb7854372022-12-21T23:32:22ZfasShahid Sadoughi University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd2228-57412228-57332018-06-01263275285Laboratory study: evaluation and comparison of physicochemical properties, level of surface charge and cytotoxicity of lipid nanocomposites for carrying nucleic acid in bone cancerFatemeh Barzegari firouzabadi0Shahrbanoo Oryan1Mohammad Hasan Sheikhha2Seyed Mehdi Kalantar3Ameneh javed4 Kharazmi University Kharazmi University Shahid Sadoughi University of Medical Sciences Shahid Sadoughi University of Medical Sciences Science and Art University Introdution: The aim of this study was to investigate the physico-chemical properties, surface charge and cytotoxicity of nanocomposite formulations for the design of lipid nano-systems with maximum loading for nucleic acids in bone marrow cancer. Methods For this study, six types of liposomes with different compositions were synthesized using DPPC, cholesterol, DSPE-mPEG (2000) and DOTAP. Then, nanosystems were evaluated for particle size, surface charge, cytotoxicity and loading rate of miRNA in two MG-63 and SaOs2 cell lines, which are human cell bone marrow cell lines. Results: All nano-systems were monodisperse. Among the systems, the F6 formula has the lowest toxicity and the highest load of miRNA-143, due to the presence of a suitable amount of DOTAP and PEG phospholipid in the liposome structure. Conclusion: Liposomal formulation with a suitable percentage of DOTAP cationic phospholipid can be used as a successful carrier for the transport of miRNA in the gene therapy process for the treatment of various cancers, especially metastatic types.http://jssu.ssu.ac.ir/browse.php?a_code=A-10-303-4&slc_lang=en&sid=1miRNA Nanocomposite Gene therapy Cell cytotoxicity |
| spellingShingle | Fatemeh Barzegari firouzabadi Shahrbanoo Oryan Mohammad Hasan Sheikhha Seyed Mehdi Kalantar Ameneh javed Laboratory study: evaluation and comparison of physicochemical properties, level of surface charge and cytotoxicity of lipid nanocomposites for carrying nucleic acid in bone cancer Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd miRNA Nanocomposite Gene therapy Cell cytotoxicity |
| title | Laboratory study: evaluation and comparison of physicochemical properties, level of surface charge and cytotoxicity of lipid nanocomposites for carrying nucleic acid in bone cancer |
| title_full | Laboratory study: evaluation and comparison of physicochemical properties, level of surface charge and cytotoxicity of lipid nanocomposites for carrying nucleic acid in bone cancer |
| title_fullStr | Laboratory study: evaluation and comparison of physicochemical properties, level of surface charge and cytotoxicity of lipid nanocomposites for carrying nucleic acid in bone cancer |
| title_full_unstemmed | Laboratory study: evaluation and comparison of physicochemical properties, level of surface charge and cytotoxicity of lipid nanocomposites for carrying nucleic acid in bone cancer |
| title_short | Laboratory study: evaluation and comparison of physicochemical properties, level of surface charge and cytotoxicity of lipid nanocomposites for carrying nucleic acid in bone cancer |
| title_sort | laboratory study evaluation and comparison of physicochemical properties level of surface charge and cytotoxicity of lipid nanocomposites for carrying nucleic acid in bone cancer |
| topic | miRNA Nanocomposite Gene therapy Cell cytotoxicity |
| url | http://jssu.ssu.ac.ir/browse.php?a_code=A-10-303-4&slc_lang=en&sid=1 |
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