Hesperidin as a preventive resistance agent in MCF–7 breast cancer cells line resistance to doxorubicin
Objective: To evaluate of hesperidin to overcome resistance of doxorubicin in MCF-7 resistant doxorubicin cells (MCF-7/Dox) in cytotoxicity apoptosis and P-glycoprotein (Pgp) expression in combination with doxorubicin. Methods: The cytotoxic properties, 50% inhibition concentration (IC50) and its co...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-03-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169115301970 |
| _version_ | 1828516626076532736 |
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| author | Rifki Febriansah Dewi P.P. Dyaningtyas Sarmoko Nunuk Aries Nurulita Edy Meiyanto Agung Endro Nugroho |
| author_facet | Rifki Febriansah Dewi P.P. Dyaningtyas Sarmoko Nunuk Aries Nurulita Edy Meiyanto Agung Endro Nugroho |
| author_sort | Rifki Febriansah |
| collection | DOAJ |
| description | Objective: To evaluate of hesperidin to overcome resistance of doxorubicin in MCF-7 resistant doxorubicin cells (MCF-7/Dox) in cytotoxicity apoptosis and P-glycoprotein (Pgp) expression in combination with doxorubicin.
Methods: The cytotoxic properties, 50% inhibition concentration (IC50) and its combination with doxorubicin in MCF-7 cell lines resistant to doxorubicin (MCF-7/Dox) cells were determined using MTT assay. Apoptosis induction was examined by double staining assay using ethidium bromide-acridine orange. Immunocytochemistry assay was performed to determine the level and localization of Pgp.
Results: Single treatment of hesperidin showed cytotoxic activity on MCF-7/Dox cells with IC50 value of 11 μmol/L. Thus, combination treatment from hesperidin and doxorubicin showed addictive and antagonist effect (CI>1.0). Hesperidin did not increase the apoptotic induction, but decreased the Pgp expressions level when combined with doxorubicin in low concentration.
Conclusions: Hesperidin has cytotoxic effect on MCF-7/Dox cells with IC50 of 11 μmol/L. Hesperidin did not increased the apoptotic induction combined with doxorubicin. Co-chemotherapy application of doxorubicin and hesperidin on MCF-7/Dox cells showed synergism effect through inhibition of Pgp expression. |
| first_indexed | 2024-12-11T18:29:36Z |
| format | Article |
| id | doaj.art-3863d9f0c51546b4a42ca2d1b8c74c06 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-11T18:29:36Z |
| publishDate | 2014-03-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-3863d9f0c51546b4a42ca2d1b8c74c062022-12-22T00:54:57ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912014-03-014322823310.1016/S2221-1691(14)60236-7Hesperidin as a preventive resistance agent in MCF–7 breast cancer cells line resistance to doxorubicinRifki Febriansah0Dewi P.P. Dyaningtyas1Sarmoko2Nunuk Aries Nurulita3Edy Meiyanto4Agung Endro Nugroho5Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta, IndonesiaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta, IndonesiaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta, IndonesiaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta, IndonesiaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta, IndonesiaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta, IndonesiaObjective: To evaluate of hesperidin to overcome resistance of doxorubicin in MCF-7 resistant doxorubicin cells (MCF-7/Dox) in cytotoxicity apoptosis and P-glycoprotein (Pgp) expression in combination with doxorubicin. Methods: The cytotoxic properties, 50% inhibition concentration (IC50) and its combination with doxorubicin in MCF-7 cell lines resistant to doxorubicin (MCF-7/Dox) cells were determined using MTT assay. Apoptosis induction was examined by double staining assay using ethidium bromide-acridine orange. Immunocytochemistry assay was performed to determine the level and localization of Pgp. Results: Single treatment of hesperidin showed cytotoxic activity on MCF-7/Dox cells with IC50 value of 11 μmol/L. Thus, combination treatment from hesperidin and doxorubicin showed addictive and antagonist effect (CI>1.0). Hesperidin did not increase the apoptotic induction, but decreased the Pgp expressions level when combined with doxorubicin in low concentration. Conclusions: Hesperidin has cytotoxic effect on MCF-7/Dox cells with IC50 of 11 μmol/L. Hesperidin did not increased the apoptotic induction combined with doxorubicin. Co-chemotherapy application of doxorubicin and hesperidin on MCF-7/Dox cells showed synergism effect through inhibition of Pgp expression.http://www.sciencedirect.com/science/article/pii/S2221169115301970HesperidinDoxorubicinMCF-7/Dox cells lineApoptosisPgp expression |
| spellingShingle | Rifki Febriansah Dewi P.P. Dyaningtyas Sarmoko Nunuk Aries Nurulita Edy Meiyanto Agung Endro Nugroho Hesperidin as a preventive resistance agent in MCF–7 breast cancer cells line resistance to doxorubicin Asian Pacific Journal of Tropical Biomedicine Hesperidin Doxorubicin MCF-7/Dox cells line Apoptosis Pgp expression |
| title | Hesperidin as a preventive resistance agent in MCF–7 breast cancer cells line resistance to doxorubicin |
| title_full | Hesperidin as a preventive resistance agent in MCF–7 breast cancer cells line resistance to doxorubicin |
| title_fullStr | Hesperidin as a preventive resistance agent in MCF–7 breast cancer cells line resistance to doxorubicin |
| title_full_unstemmed | Hesperidin as a preventive resistance agent in MCF–7 breast cancer cells line resistance to doxorubicin |
| title_short | Hesperidin as a preventive resistance agent in MCF–7 breast cancer cells line resistance to doxorubicin |
| title_sort | hesperidin as a preventive resistance agent in mcf 7 breast cancer cells line resistance to doxorubicin |
| topic | Hesperidin Doxorubicin MCF-7/Dox cells line Apoptosis Pgp expression |
| url | http://www.sciencedirect.com/science/article/pii/S2221169115301970 |
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