Evolutionarily conserved sperm factors, DCST1 and DCST2, are required for gamete fusion
To trigger gamete fusion, spermatozoa need to activate the molecular machinery in which sperm IZUMO1 and oocyte JUNO (IZUMO1R) interaction plays a critical role in mammals. Although a set of factors involved in this process has recently been identified, no common factor that can function in both ver...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-04-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/66313 |
| _version_ | 1811202824083603456 |
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| author | Naokazu Inoue Yoshihisa Hagihara Ikuo Wada |
| author_facet | Naokazu Inoue Yoshihisa Hagihara Ikuo Wada |
| author_sort | Naokazu Inoue |
| collection | DOAJ |
| description | To trigger gamete fusion, spermatozoa need to activate the molecular machinery in which sperm IZUMO1 and oocyte JUNO (IZUMO1R) interaction plays a critical role in mammals. Although a set of factors involved in this process has recently been identified, no common factor that can function in both vertebrates and invertebrates has yet been reported. Here, we first demonstrate that the evolutionarily conserved factors dendrocyte expressed seven transmembrane protein domain-containing 1 (DCST1) and dendrocyte expressed seven transmembrane protein domain-containing 2 (DCST2) are essential for sperm–egg fusion in mice, as proven by gene disruption and complementation experiments. We also found that the protein stability of another gamete fusion-related sperm factor, SPACA6, is differently regulated by DCST1/2 and IZUMO1. Thus, we suggest that spermatozoa ensure proper fertilization in mammals by integrating various molecular pathways, including an evolutionarily conserved system that has developed as a result of nearly one billion years of evolution. |
| first_indexed | 2024-04-12T02:46:08Z |
| format | Article |
| id | doaj.art-38665ed4db844b6b9e91c46d39fe6da1 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:46:08Z |
| publishDate | 2021-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-38665ed4db844b6b9e91c46d39fe6da12022-12-22T03:51:10ZengeLife Sciences Publications LtdeLife2050-084X2021-04-011010.7554/eLife.66313Evolutionarily conserved sperm factors, DCST1 and DCST2, are required for gamete fusionNaokazu Inoue0https://orcid.org/0000-0002-6187-8293Yoshihisa Hagihara1https://orcid.org/0000-0002-5980-1764Ikuo Wada2https://orcid.org/0000-0001-5668-6994Department of Cell Science, Institute of Biomedical Sciences, School of Medicine, Fukushima Medical University, Fukushima, JapanBiomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, JapanDepartment of Cell Science, Institute of Biomedical Sciences, School of Medicine, Fukushima Medical University, Fukushima, JapanTo trigger gamete fusion, spermatozoa need to activate the molecular machinery in which sperm IZUMO1 and oocyte JUNO (IZUMO1R) interaction plays a critical role in mammals. Although a set of factors involved in this process has recently been identified, no common factor that can function in both vertebrates and invertebrates has yet been reported. Here, we first demonstrate that the evolutionarily conserved factors dendrocyte expressed seven transmembrane protein domain-containing 1 (DCST1) and dendrocyte expressed seven transmembrane protein domain-containing 2 (DCST2) are essential for sperm–egg fusion in mice, as proven by gene disruption and complementation experiments. We also found that the protein stability of another gamete fusion-related sperm factor, SPACA6, is differently regulated by DCST1/2 and IZUMO1. Thus, we suggest that spermatozoa ensure proper fertilization in mammals by integrating various molecular pathways, including an evolutionarily conserved system that has developed as a result of nearly one billion years of evolution.https://elifesciences.org/articles/66313fertilizationspermatozoonoocytegamete fusiongamete interaction |
| spellingShingle | Naokazu Inoue Yoshihisa Hagihara Ikuo Wada Evolutionarily conserved sperm factors, DCST1 and DCST2, are required for gamete fusion eLife fertilization spermatozoon oocyte gamete fusion gamete interaction |
| title | Evolutionarily conserved sperm factors, DCST1 and DCST2, are required for gamete fusion |
| title_full | Evolutionarily conserved sperm factors, DCST1 and DCST2, are required for gamete fusion |
| title_fullStr | Evolutionarily conserved sperm factors, DCST1 and DCST2, are required for gamete fusion |
| title_full_unstemmed | Evolutionarily conserved sperm factors, DCST1 and DCST2, are required for gamete fusion |
| title_short | Evolutionarily conserved sperm factors, DCST1 and DCST2, are required for gamete fusion |
| title_sort | evolutionarily conserved sperm factors dcst1 and dcst2 are required for gamete fusion |
| topic | fertilization spermatozoon oocyte gamete fusion gamete interaction |
| url | https://elifesciences.org/articles/66313 |
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