Novel Cesium Resistance Mechanism of Alkaliphilic Bacterium Isolated From Jumping Spider Ground Extract
The radionuclide isotopes (134Cs and 137Cs) of Cesium (Cs), an alkali metal, are attracting attention as major causes of radioactive contamination. Although Cs+ is harmful to the growth of plants and bacteria, alkaliphilic bacterium Microbacterium sp. TS-1, isolated from a jumping spider, showed gro...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.841821/full |
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| author | Takahiro Koretsune Yoshiki Ishida Yuri Kaneda Eri Ishiuchi Miyu Teshima Nanami Marubashi Katsuya Satoh Masahiro Ito Masahiro Ito Masahiro Ito Masahiro Ito |
| author_facet | Takahiro Koretsune Yoshiki Ishida Yuri Kaneda Eri Ishiuchi Miyu Teshima Nanami Marubashi Katsuya Satoh Masahiro Ito Masahiro Ito Masahiro Ito Masahiro Ito |
| author_sort | Takahiro Koretsune |
| collection | DOAJ |
| description | The radionuclide isotopes (134Cs and 137Cs) of Cesium (Cs), an alkali metal, are attracting attention as major causes of radioactive contamination. Although Cs+ is harmful to the growth of plants and bacteria, alkaliphilic bacterium Microbacterium sp. TS-1, isolated from a jumping spider, showed growth even in the presence of 1.2 M CsCl. The maximum concentration of Cs+ that microorganisms can withstand has been reported to be 700 mM till date, suggesting that the strain TS-1 is resistant to a high concentration of Cs ions. Multiple reports of cesium ion-resistant bacteria have been reported, but the detailed mechanism has not yet been elucidated. We obtained Cs ion-sensitive mutants and their revertant mutants from strain TS-1 and identified a Cs ion resistance-related gene, MTS1_00475, by performing SNP analysis of the whole-genome sequence data. When exposed to more than 200 mM Cs+ concentration, the intracellular Cs+ concentration was constantly lowered by MTS1_00475, which encodes the novel low-affinity Cs+/H+ antiporter. This study is the first to clarify the mechanism of cesium resistance in unexplained cesium-resistant microorganisms. By clarifying the new cesium resistance mechanism, it can be expected to be used as a bioremediation tool for treating radioactive Cs+ contaminated water. |
| first_indexed | 2024-12-10T09:20:07Z |
| format | Article |
| id | doaj.art-386692947efb4b70aba13c33daf396ae |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T09:20:07Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-386692947efb4b70aba13c33daf396ae2022-12-22T01:54:43ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-03-011310.3389/fmicb.2022.841821841821Novel Cesium Resistance Mechanism of Alkaliphilic Bacterium Isolated From Jumping Spider Ground ExtractTakahiro Koretsune0Yoshiki Ishida1Yuri Kaneda2Eri Ishiuchi3Miyu Teshima4Nanami Marubashi5Katsuya Satoh6Masahiro Ito7Masahiro Ito8Masahiro Ito9Masahiro Ito10Graduate School of Life Sciences, Toyo University, Oura-gun, JapanGraduate School of Life Sciences, Toyo University, Oura-gun, JapanFaculty of Life Sciences, Toyo University, Oura-gun, JapanFaculty of Life Sciences, Toyo University, Oura-gun, JapanFaculty of Life Sciences, Toyo University, Oura-gun, JapanFaculty of Life Sciences, Toyo University, Oura-gun, JapanDepartment of Radiation-Applied Biology Research, Takasaki Advanced Radiation Research Institute, Quantum Beam Science Research Directorate, National Institutes for Quantum Science and Technology, Takasaki, JapanGraduate School of Life Sciences, Toyo University, Oura-gun, JapanFaculty of Life Sciences, Toyo University, Oura-gun, JapanBio-Nano Electronics Research Center, Toyo University, Kawagoe, JapanBio-Resilience Research Project (BRRP), Toyo University, Oura-gun, JapanThe radionuclide isotopes (134Cs and 137Cs) of Cesium (Cs), an alkali metal, are attracting attention as major causes of radioactive contamination. Although Cs+ is harmful to the growth of plants and bacteria, alkaliphilic bacterium Microbacterium sp. TS-1, isolated from a jumping spider, showed growth even in the presence of 1.2 M CsCl. The maximum concentration of Cs+ that microorganisms can withstand has been reported to be 700 mM till date, suggesting that the strain TS-1 is resistant to a high concentration of Cs ions. Multiple reports of cesium ion-resistant bacteria have been reported, but the detailed mechanism has not yet been elucidated. We obtained Cs ion-sensitive mutants and their revertant mutants from strain TS-1 and identified a Cs ion resistance-related gene, MTS1_00475, by performing SNP analysis of the whole-genome sequence data. When exposed to more than 200 mM Cs+ concentration, the intracellular Cs+ concentration was constantly lowered by MTS1_00475, which encodes the novel low-affinity Cs+/H+ antiporter. This study is the first to clarify the mechanism of cesium resistance in unexplained cesium-resistant microorganisms. By clarifying the new cesium resistance mechanism, it can be expected to be used as a bioremediation tool for treating radioactive Cs+ contaminated water.https://www.frontiersin.org/articles/10.3389/fmicb.2022.841821/fullcesium-resistant microorganismsMicrobacteriumalkaliphilicmutantwhole-genome sequencing |
| spellingShingle | Takahiro Koretsune Yoshiki Ishida Yuri Kaneda Eri Ishiuchi Miyu Teshima Nanami Marubashi Katsuya Satoh Masahiro Ito Masahiro Ito Masahiro Ito Masahiro Ito Novel Cesium Resistance Mechanism of Alkaliphilic Bacterium Isolated From Jumping Spider Ground Extract Frontiers in Microbiology cesium-resistant microorganisms Microbacterium alkaliphilic mutant whole-genome sequencing |
| title | Novel Cesium Resistance Mechanism of Alkaliphilic Bacterium Isolated From Jumping Spider Ground Extract |
| title_full | Novel Cesium Resistance Mechanism of Alkaliphilic Bacterium Isolated From Jumping Spider Ground Extract |
| title_fullStr | Novel Cesium Resistance Mechanism of Alkaliphilic Bacterium Isolated From Jumping Spider Ground Extract |
| title_full_unstemmed | Novel Cesium Resistance Mechanism of Alkaliphilic Bacterium Isolated From Jumping Spider Ground Extract |
| title_short | Novel Cesium Resistance Mechanism of Alkaliphilic Bacterium Isolated From Jumping Spider Ground Extract |
| title_sort | novel cesium resistance mechanism of alkaliphilic bacterium isolated from jumping spider ground extract |
| topic | cesium-resistant microorganisms Microbacterium alkaliphilic mutant whole-genome sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.841821/full |
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