SK-03-92 Drug Kills Intracellular <i>Mycobacterium tuberculosis</i>
Background: Tuberculosis affects millions of people worldwide. The emergence of drug-resistant <i>Mycobacterium tuberculosis</i> strains has made treatment more difficult. A drug discovery project initiated to screen natural products identified a lead stilbene compound, and structure fun...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/9/1385 |
| _version_ | 1797581551344549888 |
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| author | William R. Schwan |
| author_facet | William R. Schwan |
| author_sort | William R. Schwan |
| collection | DOAJ |
| description | Background: Tuberculosis affects millions of people worldwide. The emergence of drug-resistant <i>Mycobacterium tuberculosis</i> strains has made treatment more difficult. A drug discovery project initiated to screen natural products identified a lead stilbene compound, and structure function analysis of hundreds of analogs led to the discovery of the SK-03-92 stilbene lead compound with activity against several non-tuberculoid mycobacteria. Methods: For this study, an MIC analysis and intracellular killing assay were performed to test SK-03-92 against <i>M. tuberculosis</i> grown in vitro as well as within murine macrophage cells. Results: The MIC analysis showed that SK-03-92 had activity against <i>M. tuberculosis</i> in the range of 0.39 to 6.25 μg/mL, including activity against single-drug-resistant strains. Further, an intracellular kill assay demonstrated that the SK-03-92 lead compound killed <i>M. tuberculosis</i> cells within murine macrophage cells. Conclusion: Together, the data show the SK-03-92 lead compound can kill <i>M. tuberculosis</i> bacteria within mammalian macrophages. |
| first_indexed | 2024-03-10T23:06:15Z |
| format | Article |
| id | doaj.art-3867fd724c0d4fabaf1f50816376f150 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T23:06:15Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-3867fd724c0d4fabaf1f50816376f1502023-11-19T09:17:02ZengMDPI AGAntibiotics2079-63822023-08-01129138510.3390/antibiotics12091385SK-03-92 Drug Kills Intracellular <i>Mycobacterium tuberculosis</i>William R. Schwan0Department of Microbiology, University of Wisconsin-La Crosse, 1725 State St., La Crosse, WI 54601, USABackground: Tuberculosis affects millions of people worldwide. The emergence of drug-resistant <i>Mycobacterium tuberculosis</i> strains has made treatment more difficult. A drug discovery project initiated to screen natural products identified a lead stilbene compound, and structure function analysis of hundreds of analogs led to the discovery of the SK-03-92 stilbene lead compound with activity against several non-tuberculoid mycobacteria. Methods: For this study, an MIC analysis and intracellular killing assay were performed to test SK-03-92 against <i>M. tuberculosis</i> grown in vitro as well as within murine macrophage cells. Results: The MIC analysis showed that SK-03-92 had activity against <i>M. tuberculosis</i> in the range of 0.39 to 6.25 μg/mL, including activity against single-drug-resistant strains. Further, an intracellular kill assay demonstrated that the SK-03-92 lead compound killed <i>M. tuberculosis</i> cells within murine macrophage cells. Conclusion: Together, the data show the SK-03-92 lead compound can kill <i>M. tuberculosis</i> bacteria within mammalian macrophages.https://www.mdpi.com/2079-6382/12/9/1385SK-03-92<i>Mycobacterium tuberculosis</i>stilbenemacrophage |
| spellingShingle | William R. Schwan SK-03-92 Drug Kills Intracellular <i>Mycobacterium tuberculosis</i> Antibiotics SK-03-92 <i>Mycobacterium tuberculosis</i> stilbene macrophage |
| title | SK-03-92 Drug Kills Intracellular <i>Mycobacterium tuberculosis</i> |
| title_full | SK-03-92 Drug Kills Intracellular <i>Mycobacterium tuberculosis</i> |
| title_fullStr | SK-03-92 Drug Kills Intracellular <i>Mycobacterium tuberculosis</i> |
| title_full_unstemmed | SK-03-92 Drug Kills Intracellular <i>Mycobacterium tuberculosis</i> |
| title_short | SK-03-92 Drug Kills Intracellular <i>Mycobacterium tuberculosis</i> |
| title_sort | sk 03 92 drug kills intracellular i mycobacterium tuberculosis i |
| topic | SK-03-92 <i>Mycobacterium tuberculosis</i> stilbene macrophage |
| url | https://www.mdpi.com/2079-6382/12/9/1385 |
| work_keys_str_mv | AT williamrschwan sk0392drugkillsintracellularimycobacteriumtuberculosisi |