First Record of Spirometra erinaceieuropaei spargana Infection in Amphibians of South Asia: A Causative Agent of Surficial Abnormalities
Background: Sparganosis is a zoonotic disease caused by Plerocercoid larvae (spargana) of the genus Spirometra. We aimed to provide molecular evidence for the infection of amphibians with Spirometra sp. in the inside and outside of Horton Plains National Park (HPNP), Sri Lanka. Methods: The prev...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2022-09-01
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| Series: | Iranian Journal of Parasitology |
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| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3411 |
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| author | Praneeth Silva Tharanga Dasanayake Dulan Jayasekara Chathuranga Dharmarathne Dharshani Mahaulpatha |
| author_facet | Praneeth Silva Tharanga Dasanayake Dulan Jayasekara Chathuranga Dharmarathne Dharshani Mahaulpatha |
| author_sort | Praneeth Silva |
| collection | DOAJ |
| description |
Background: Sparganosis is a zoonotic disease caused by Plerocercoid larvae (spargana) of the genus Spirometra. We aimed to provide molecular evidence for the infection of amphibians with Spirometra sp. in the inside and outside of Horton Plains National Park (HPNP), Sri Lanka.
Methods: The prevalence of sparganum infection in wild frogs (Truga eques and Minverya agricola) was investigated in the inside and outside of HPNP from June 2019 to April 2021.A total of 1,434 Amphibians samples were surveyed to examine the spargana infection from the study site. To identify the species identity of the collected spargana, a portion of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene and nuclear 18S rRNA gene were amplified, sequenced, and analyzed.
Results: A total of 539 infected amphibians (T. eques and M. agricola) samples were examined to survey from the study area. Spargana were detected in all dissected specimens belonging to the species Spirometra erinaceieuropaei that were genetically confirmed using the evolutionary conserved nuclear 18S rRNA gene and then compared to the GenBank deposit, indicating that S. erinaceieuropaei is the primary causal agent of sparganosis both inside and outside the HPNP.
Conclusion: Our finding is the first genetically confirmed record of S. erinaceieuropaei in amphibians in South Asia. However, further studies are needed to investigate the prevalence of sparagna infection in amphibians all over the island.
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| first_indexed | 2024-04-11T20:03:20Z |
| format | Article |
| id | doaj.art-386a181706384b3caac15693d2f85b7b |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-04-11T20:03:20Z |
| publishDate | 2022-09-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-386a181706384b3caac15693d2f85b7b2022-12-22T04:05:25ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2022-09-0117310.18502/ijpa.v17i3.10631First Record of Spirometra erinaceieuropaei spargana Infection in Amphibians of South Asia: A Causative Agent of Surficial AbnormalitiesPraneeth Silva0Tharanga Dasanayake1Dulan Jayasekara2Chathuranga Dharmarathne3Dharshani Mahaulpatha4Department of Oral Biology, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Zoology, Faculty of Applied Sciences, University of Sri Jayewardenepura, Nugegoda, Sri LankaDepartment of Zoology, Faculty of Applied Sciences, University of Sri Jayewardenepura, Nugegoda, Sri LankaDepartment of Biological Sciences and Engineering, Macquarie University, Sydney, AustraliaDepartment of Zoology, Faculty of Applied Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka Background: Sparganosis is a zoonotic disease caused by Plerocercoid larvae (spargana) of the genus Spirometra. We aimed to provide molecular evidence for the infection of amphibians with Spirometra sp. in the inside and outside of Horton Plains National Park (HPNP), Sri Lanka. Methods: The prevalence of sparganum infection in wild frogs (Truga eques and Minverya agricola) was investigated in the inside and outside of HPNP from June 2019 to April 2021.A total of 1,434 Amphibians samples were surveyed to examine the spargana infection from the study site. To identify the species identity of the collected spargana, a portion of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene and nuclear 18S rRNA gene were amplified, sequenced, and analyzed. Results: A total of 539 infected amphibians (T. eques and M. agricola) samples were examined to survey from the study area. Spargana were detected in all dissected specimens belonging to the species Spirometra erinaceieuropaei that were genetically confirmed using the evolutionary conserved nuclear 18S rRNA gene and then compared to the GenBank deposit, indicating that S. erinaceieuropaei is the primary causal agent of sparganosis both inside and outside the HPNP. Conclusion: Our finding is the first genetically confirmed record of S. erinaceieuropaei in amphibians in South Asia. However, further studies are needed to investigate the prevalence of sparagna infection in amphibians all over the island. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3411Horton plains national park (HPNP);SparganaSparganosisSpirometra erinaceieuropaei |
| spellingShingle | Praneeth Silva Tharanga Dasanayake Dulan Jayasekara Chathuranga Dharmarathne Dharshani Mahaulpatha First Record of Spirometra erinaceieuropaei spargana Infection in Amphibians of South Asia: A Causative Agent of Surficial Abnormalities Iranian Journal of Parasitology Horton plains national park (HPNP); Spargana Sparganosis Spirometra erinaceieuropaei |
| title | First Record of Spirometra erinaceieuropaei spargana Infection in Amphibians of South Asia: A Causative Agent of Surficial Abnormalities |
| title_full | First Record of Spirometra erinaceieuropaei spargana Infection in Amphibians of South Asia: A Causative Agent of Surficial Abnormalities |
| title_fullStr | First Record of Spirometra erinaceieuropaei spargana Infection in Amphibians of South Asia: A Causative Agent of Surficial Abnormalities |
| title_full_unstemmed | First Record of Spirometra erinaceieuropaei spargana Infection in Amphibians of South Asia: A Causative Agent of Surficial Abnormalities |
| title_short | First Record of Spirometra erinaceieuropaei spargana Infection in Amphibians of South Asia: A Causative Agent of Surficial Abnormalities |
| title_sort | first record of spirometra erinaceieuropaei spargana infection in amphibians of south asia a causative agent of surficial abnormalities |
| topic | Horton plains national park (HPNP); Spargana Sparganosis Spirometra erinaceieuropaei |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3411 |
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