Transcriptome Analysis of the Cerebellum of Mice Fed a Manganese-Deficient Diet
Manganese (Mn), primarily acquired through diet, is required for brain function and development. Epidemiological studies have found an association between both low and high levels of Mn and impaired neurodevelopment in children. Recent genetic studies have revealed that patients with congenital Mn d...
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Frontiers Media S.A.
2020-12-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2020.558725/full |
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| author | Young Ah Seo Eun-Kyung Choi Luisa Aring Molly Paschall Shigeki Iwase |
| author_facet | Young Ah Seo Eun-Kyung Choi Luisa Aring Molly Paschall Shigeki Iwase |
| author_sort | Young Ah Seo |
| collection | DOAJ |
| description | Manganese (Mn), primarily acquired through diet, is required for brain function and development. Epidemiological studies have found an association between both low and high levels of Mn and impaired neurodevelopment in children. Recent genetic studies have revealed that patients with congenital Mn deficiency display severe psychomotor disability and cerebral and cerebellar atrophy. Although the impact of Mn on gene expression is beginning to be appreciated, Mn-dependent gene expression remains to be explored in vertebrate animals. The goal of this study was to use a mouse model to define the impact of a low-Mn diet on brain metal levels and gene expression. We interrogated gene expression changes in the Mn-deficient mouse brain at the genome-wide scale by RNA-seq analysis of the cerebellum of mice fed low or normal Mn diets. A total of 137 genes were differentially expressed in Mn-deficient cerebellums compared with Mn-adequate cerebellums (Padj < 0.05). Mn-deficient mice displayed downregulation of key pathways involved with “focal adhesion,” “neuroactive ligand-receptor interaction,” and “cytokine-cytokine receptor interaction” and upregulation of “herpes simplex virus 1 infection,” “spliceosome,” and “FoxO signaling pathway.” Reactome pathway analysis identified upregulation of the splicing-related pathways and transcription-related pathways, as well as downregulation of “metabolism of carbohydrate,” and “extracellular matrix organization,” and “fatty acid metabolism” reactomes. The recurrent identifications of splicing-related pathways suggest that Mn deficiency leads to upregulation of splicing machineries and downregulation of diverse biological pathways. |
| first_indexed | 2024-12-16T20:06:55Z |
| format | Article |
| id | doaj.art-386b063897644784bfc24d78f229617c |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-16T20:06:55Z |
| publishDate | 2020-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-386b063897644784bfc24d78f229617c2022-12-21T22:18:17ZengFrontiers Media S.A.Frontiers in Genetics1664-80212020-12-011110.3389/fgene.2020.558725558725Transcriptome Analysis of the Cerebellum of Mice Fed a Manganese-Deficient DietYoung Ah Seo0Eun-Kyung Choi1Luisa Aring2Molly Paschall3Shigeki Iwase4Department of Nutritional Sciences, University of Michigan School of Public Health, Ann Arbor, MI, United StatesDepartment of Nutritional Sciences, University of Michigan School of Public Health, Ann Arbor, MI, United StatesDepartment of Nutritional Sciences, University of Michigan School of Public Health, Ann Arbor, MI, United StatesDepartment of Nutritional Sciences, University of Michigan School of Public Health, Ann Arbor, MI, United StatesDepartment of Human Genetics, Michigan Medicine, University of Michigan, Ann Arbor, MI, United StatesManganese (Mn), primarily acquired through diet, is required for brain function and development. Epidemiological studies have found an association between both low and high levels of Mn and impaired neurodevelopment in children. Recent genetic studies have revealed that patients with congenital Mn deficiency display severe psychomotor disability and cerebral and cerebellar atrophy. Although the impact of Mn on gene expression is beginning to be appreciated, Mn-dependent gene expression remains to be explored in vertebrate animals. The goal of this study was to use a mouse model to define the impact of a low-Mn diet on brain metal levels and gene expression. We interrogated gene expression changes in the Mn-deficient mouse brain at the genome-wide scale by RNA-seq analysis of the cerebellum of mice fed low or normal Mn diets. A total of 137 genes were differentially expressed in Mn-deficient cerebellums compared with Mn-adequate cerebellums (Padj < 0.05). Mn-deficient mice displayed downregulation of key pathways involved with “focal adhesion,” “neuroactive ligand-receptor interaction,” and “cytokine-cytokine receptor interaction” and upregulation of “herpes simplex virus 1 infection,” “spliceosome,” and “FoxO signaling pathway.” Reactome pathway analysis identified upregulation of the splicing-related pathways and transcription-related pathways, as well as downregulation of “metabolism of carbohydrate,” and “extracellular matrix organization,” and “fatty acid metabolism” reactomes. The recurrent identifications of splicing-related pathways suggest that Mn deficiency leads to upregulation of splicing machineries and downregulation of diverse biological pathways.https://www.frontiersin.org/articles/10.3389/fgene.2020.558725/fullmanganesecerebellumtranscriptomeneurodevelopmentspliceosome |
| spellingShingle | Young Ah Seo Eun-Kyung Choi Luisa Aring Molly Paschall Shigeki Iwase Transcriptome Analysis of the Cerebellum of Mice Fed a Manganese-Deficient Diet Frontiers in Genetics manganese cerebellum transcriptome neurodevelopment spliceosome |
| title | Transcriptome Analysis of the Cerebellum of Mice Fed a Manganese-Deficient Diet |
| title_full | Transcriptome Analysis of the Cerebellum of Mice Fed a Manganese-Deficient Diet |
| title_fullStr | Transcriptome Analysis of the Cerebellum of Mice Fed a Manganese-Deficient Diet |
| title_full_unstemmed | Transcriptome Analysis of the Cerebellum of Mice Fed a Manganese-Deficient Diet |
| title_short | Transcriptome Analysis of the Cerebellum of Mice Fed a Manganese-Deficient Diet |
| title_sort | transcriptome analysis of the cerebellum of mice fed a manganese deficient diet |
| topic | manganese cerebellum transcriptome neurodevelopment spliceosome |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2020.558725/full |
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