Molecular Method Is Essential to Identify Asymptomatic Malaria Reservoirs: A Successful Experience in the Malaria Elimination Program in Iran
<b>Background:</b> The accurate diagnosis of malaria cases, especially asymptotic and low-parasitemia patients, using robust molecular methods (nested-PCR) have been emphasized. The goal of this study was to detect active cases of malaria in areas with a history of local malaria transmis...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/12/3025 |
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| author | Jebreil Shamseddin Amin Ghanbarnejad Abdoljabbar Zakeri Farshid Abedi Shaghayegh Khojasteh Habibollah Turki |
| author_facet | Jebreil Shamseddin Amin Ghanbarnejad Abdoljabbar Zakeri Farshid Abedi Shaghayegh Khojasteh Habibollah Turki |
| author_sort | Jebreil Shamseddin |
| collection | DOAJ |
| description | <b>Background:</b> The accurate diagnosis of malaria cases, especially asymptotic and low-parasitemia patients, using robust molecular methods (nested-PCR) have been emphasized. The goal of this study was to detect active cases of malaria in areas with a history of local malaria transmission focusing on the use of molecular tools to ensure that the malaria elimination program has been implemented successfully. <b>Methods:</b> In this cross-sectional study, 816 blood samples were taken from immigrants and local residents of malaria-endemic areas in Hormozgan province, Iran. In order to identify asymptomatic malaria parasite reservoirs, the samples were examined using microscopic, RDT, and nested-PCR techniques. <b>Results:</b> About twelve positive asymptomatic malaria cases were identified when the molecular method (nested-PCR) was used. The positivity rates among immigrants and local residents were 2.07% and 0.93%, respectively. No positive cases were detected using microscopic and RDT methods. <b>Conclusions:</b> The finding of the research emphasize that in addition to microscopy and RDTs methods, sensitive molecular tools as a standard and essential strategy are needed in the diagnosis and detection of asymptomatic parasite reservoir. |
| first_indexed | 2024-03-09T17:08:11Z |
| format | Article |
| id | doaj.art-386b491ec0664ae5b3c0dacffce2a8d2 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T17:08:11Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-386b491ec0664ae5b3c0dacffce2a8d22023-11-24T14:17:22ZengMDPI AGDiagnostics2075-44182022-12-011212302510.3390/diagnostics12123025Molecular Method Is Essential to Identify Asymptomatic Malaria Reservoirs: A Successful Experience in the Malaria Elimination Program in IranJebreil Shamseddin0Amin Ghanbarnejad1Abdoljabbar Zakeri2Farshid Abedi3Shaghayegh Khojasteh4Habibollah Turki5Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas 7916613885, IranSocial Determinants in Health Promotion Research Center, Hormozgan Health Institute, Hormozgan, University of Medical Sciences, Bandar Abbas 7919916753, IranInfectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas 7919693116, IranInfectious Diseases Research Center, Birjand University of Medical Sciences, Birjand 9717853577, IranMolecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas 7916613885, IranMolecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas 7916613885, Iran<b>Background:</b> The accurate diagnosis of malaria cases, especially asymptotic and low-parasitemia patients, using robust molecular methods (nested-PCR) have been emphasized. The goal of this study was to detect active cases of malaria in areas with a history of local malaria transmission focusing on the use of molecular tools to ensure that the malaria elimination program has been implemented successfully. <b>Methods:</b> In this cross-sectional study, 816 blood samples were taken from immigrants and local residents of malaria-endemic areas in Hormozgan province, Iran. In order to identify asymptomatic malaria parasite reservoirs, the samples were examined using microscopic, RDT, and nested-PCR techniques. <b>Results:</b> About twelve positive asymptomatic malaria cases were identified when the molecular method (nested-PCR) was used. The positivity rates among immigrants and local residents were 2.07% and 0.93%, respectively. No positive cases were detected using microscopic and RDT methods. <b>Conclusions:</b> The finding of the research emphasize that in addition to microscopy and RDTs methods, sensitive molecular tools as a standard and essential strategy are needed in the diagnosis and detection of asymptomatic parasite reservoir.https://www.mdpi.com/2075-4418/12/12/3025malariadiagnosticmolecular techniqueslow-density infectioncase detectionelimination program |
| spellingShingle | Jebreil Shamseddin Amin Ghanbarnejad Abdoljabbar Zakeri Farshid Abedi Shaghayegh Khojasteh Habibollah Turki Molecular Method Is Essential to Identify Asymptomatic Malaria Reservoirs: A Successful Experience in the Malaria Elimination Program in Iran Diagnostics malaria diagnostic molecular techniques low-density infection case detection elimination program |
| title | Molecular Method Is Essential to Identify Asymptomatic Malaria Reservoirs: A Successful Experience in the Malaria Elimination Program in Iran |
| title_full | Molecular Method Is Essential to Identify Asymptomatic Malaria Reservoirs: A Successful Experience in the Malaria Elimination Program in Iran |
| title_fullStr | Molecular Method Is Essential to Identify Asymptomatic Malaria Reservoirs: A Successful Experience in the Malaria Elimination Program in Iran |
| title_full_unstemmed | Molecular Method Is Essential to Identify Asymptomatic Malaria Reservoirs: A Successful Experience in the Malaria Elimination Program in Iran |
| title_short | Molecular Method Is Essential to Identify Asymptomatic Malaria Reservoirs: A Successful Experience in the Malaria Elimination Program in Iran |
| title_sort | molecular method is essential to identify asymptomatic malaria reservoirs a successful experience in the malaria elimination program in iran |
| topic | malaria diagnostic molecular techniques low-density infection case detection elimination program |
| url | https://www.mdpi.com/2075-4418/12/12/3025 |
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