Fungi diversity in PM<sub>2. 5</sub> and PM<sub>1</sub> at the summit of Mt. Tai: abundance, size distribution, and seasonal variation
Fungi are ubiquitous throughout the near-surface atmosphere, where they represent an important component of primary biological aerosol particles. This study combined internal transcribed spacer region sequencing and quantitative real-time polymerase chain reaction (qPCR) to investigate the ambie...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2017-09-01
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| Series: | Atmospheric Chemistry and Physics |
| Online Access: | https://www.atmos-chem-phys.net/17/11247/2017/acp-17-11247-2017.pdf |
| _version_ | 1818841553426186240 |
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| author | C. Xu M. Wei M. Wei J. Chen J. Chen J. Chen C. Zhu J. Li G. Lv X. Xu L. Zheng G. Sui W. Li B. Chen W. Wang Q. Zhang A. Ding A. Mellouki A. Mellouki |
| author_facet | C. Xu M. Wei M. Wei J. Chen J. Chen J. Chen C. Zhu J. Li G. Lv X. Xu L. Zheng G. Sui W. Li B. Chen W. Wang Q. Zhang A. Ding A. Mellouki A. Mellouki |
| author_sort | C. Xu |
| collection | DOAJ |
| description | Fungi are ubiquitous throughout the near-surface atmosphere, where they
represent an important component of primary biological aerosol particles.
This study combined internal transcribed spacer region sequencing and
quantitative real-time polymerase chain reaction (qPCR) to investigate the
ambient fungi in fine (PM<sub>2. 5</sub>, 50 % cutoff aerodynamic diameter
<i>D</i><sub>a50</sub> = 2.5 µm, geometric standard deviation of
collection efficiency <i>σ</i><sub><i>g</i></sub> = 1.2) and submicron (PM<sub>1</sub>,
<i>D</i><sub>a50</sub> = 1 µm, <i>σ</i><sub><i>g</i></sub> = 1.2) particles at the
summit of Mt. Tai located in the North China Plain, China. Fungal abundance
values were 9.4 × 10<sup>4</sup> and 1.3 × 10<sup>5</sup>
copies m<sup>−3</sup> in PM<sub>2. 5</sub> and PM<sub>1</sub>, respectively. Most of the fungal
sequences were from Ascomycota and Basidiomycota, which are known to actively
discharge spores into the atmosphere. The fungal community showed a
significant seasonal shift across different size fractions according to
Metastats analysis and the Kruskal–Wallis rank sum test. The abundance of
<i>Glomerella</i> and <i>Zasmidium</i> increased in larger particles in
autumn, whereas <i>Penicillium</i>, <i>Bullera</i>, and
<i>Phaeosphaeria</i> increased in smaller particles in winter.
Environmental factors, namely Ca<sup>2+</sup>, humidity, and temperature, were
found to be crucial for the seasonal variation in the fungal community. This
study might serve as an important reference for fungal contribution to
primary biological aerosol particles. |
| first_indexed | 2024-12-19T04:27:55Z |
| format | Article |
| id | doaj.art-386ca16f95ab4470bb8aec41091f6fda |
| institution | Directory Open Access Journal |
| issn | 1680-7316 1680-7324 |
| language | English |
| last_indexed | 2024-12-19T04:27:55Z |
| publishDate | 2017-09-01 |
| publisher | Copernicus Publications |
| record_format | Article |
| series | Atmospheric Chemistry and Physics |
| spelling | doaj.art-386ca16f95ab4470bb8aec41091f6fda2022-12-21T20:35:57ZengCopernicus PublicationsAtmospheric Chemistry and Physics1680-73161680-73242017-09-0117112471126010.5194/acp-17-11247-2017Fungi diversity in PM<sub>2. 5</sub> and PM<sub>1</sub> at the summit of Mt. Tai: abundance, size distribution, and seasonal variationC. Xu0M. Wei1M. Wei2J. Chen3J. Chen4J. Chen5C. Zhu6J. Li7G. Lv8X. Xu9L. Zheng10G. Sui11W. Li12B. Chen13W. Wang14Q. Zhang15A. Ding16A. Mellouki17A. Mellouki18Environment Research Institute, School of Environmental Science and Engineering, Shandong University, Jinan 250100, ChinaEnvironment Research Institute, School of Environmental Science and Engineering, Shandong University, Jinan 250100, Chinanow at: College of Geography and Environment, Shandong Normal University, Jinan 250100, ChinaEnvironment Research Institute, School of Environmental Science and Engineering, Shandong University, Jinan 250100, ChinaShanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Fudan Tyndall Centre, Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, ChinaInstitute for Climate and Global Change Research, School of Atmospheric Sciences, Nanjing University, Nanjing 210023, Jiangsu, ChinaEnvironment Research Institute, School of Environmental Science and Engineering, Shandong University, Jinan 250100, ChinaEnvironment Research Institute, School of Environmental Science and Engineering, Shandong University, Jinan 250100, ChinaEnvironment Research Institute, School of Environmental Science and Engineering, Shandong University, Jinan 250100, ChinaEnvironment Research Institute, School of Environmental Science and Engineering, Shandong University, Jinan 250100, ChinaShanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Fudan Tyndall Centre, Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, ChinaShanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Fudan Tyndall Centre, Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, ChinaEnvironment Research Institute, School of Environmental Science and Engineering, Shandong University, Jinan 250100, ChinaEnvironment Research Institute, School of Environmental Science and Engineering, Shandong University, Jinan 250100, ChinaEnvironment Research Institute, School of Environmental Science and Engineering, Shandong University, Jinan 250100, ChinaEnvironment Research Institute, School of Environmental Science and Engineering, Shandong University, Jinan 250100, ChinaInstitute for Climate and Global Change Research, School of Atmospheric Sciences, Nanjing University, Nanjing 210023, Jiangsu, ChinaEnvironment Research Institute, School of Environmental Science and Engineering, Shandong University, Jinan 250100, ChinaInstitut de Combustion, Aérothermique, Réactivité et Environnement, CNRS, 45071 Orléans CEDEX 02, FranceFungi are ubiquitous throughout the near-surface atmosphere, where they represent an important component of primary biological aerosol particles. This study combined internal transcribed spacer region sequencing and quantitative real-time polymerase chain reaction (qPCR) to investigate the ambient fungi in fine (PM<sub>2. 5</sub>, 50 % cutoff aerodynamic diameter <i>D</i><sub>a50</sub> = 2.5 µm, geometric standard deviation of collection efficiency <i>σ</i><sub><i>g</i></sub> = 1.2) and submicron (PM<sub>1</sub>, <i>D</i><sub>a50</sub> = 1 µm, <i>σ</i><sub><i>g</i></sub> = 1.2) particles at the summit of Mt. Tai located in the North China Plain, China. Fungal abundance values were 9.4 × 10<sup>4</sup> and 1.3 × 10<sup>5</sup> copies m<sup>−3</sup> in PM<sub>2. 5</sub> and PM<sub>1</sub>, respectively. Most of the fungal sequences were from Ascomycota and Basidiomycota, which are known to actively discharge spores into the atmosphere. The fungal community showed a significant seasonal shift across different size fractions according to Metastats analysis and the Kruskal–Wallis rank sum test. The abundance of <i>Glomerella</i> and <i>Zasmidium</i> increased in larger particles in autumn, whereas <i>Penicillium</i>, <i>Bullera</i>, and <i>Phaeosphaeria</i> increased in smaller particles in winter. Environmental factors, namely Ca<sup>2+</sup>, humidity, and temperature, were found to be crucial for the seasonal variation in the fungal community. This study might serve as an important reference for fungal contribution to primary biological aerosol particles.https://www.atmos-chem-phys.net/17/11247/2017/acp-17-11247-2017.pdf |
| spellingShingle | C. Xu M. Wei M. Wei J. Chen J. Chen J. Chen C. Zhu J. Li G. Lv X. Xu L. Zheng G. Sui W. Li B. Chen W. Wang Q. Zhang A. Ding A. Mellouki A. Mellouki Fungi diversity in PM<sub>2. 5</sub> and PM<sub>1</sub> at the summit of Mt. Tai: abundance, size distribution, and seasonal variation Atmospheric Chemistry and Physics |
| title | Fungi diversity in PM<sub>2. 5</sub> and PM<sub>1</sub> at the summit of Mt. Tai: abundance, size distribution, and seasonal variation |
| title_full | Fungi diversity in PM<sub>2. 5</sub> and PM<sub>1</sub> at the summit of Mt. Tai: abundance, size distribution, and seasonal variation |
| title_fullStr | Fungi diversity in PM<sub>2. 5</sub> and PM<sub>1</sub> at the summit of Mt. Tai: abundance, size distribution, and seasonal variation |
| title_full_unstemmed | Fungi diversity in PM<sub>2. 5</sub> and PM<sub>1</sub> at the summit of Mt. Tai: abundance, size distribution, and seasonal variation |
| title_short | Fungi diversity in PM<sub>2. 5</sub> and PM<sub>1</sub> at the summit of Mt. Tai: abundance, size distribution, and seasonal variation |
| title_sort | fungi diversity in pm sub 2 5 sub and pm sub 1 sub at the summit of mt tai abundance size distribution and seasonal variation |
| url | https://www.atmos-chem-phys.net/17/11247/2017/acp-17-11247-2017.pdf |
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