Molecular Insights into the Genetic Variability of ORF Virus in a Mediterranean Region (Sardinia, Italy)
Orf virus (ORFV) represents the causative agent of contagious ecthyma, clinically characterized by mild papular and pustular to severe proliferative lesions, mainly occurring in sheep and goats. In order to provide hints on the evolutionary history of this virus, we carried out a study aimed to asse...
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MDPI AG
2021-05-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/11/5/416 |
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| author | Elisabetta Coradduzza Daria Sanna Angela M. Rocchigiani Davide Pintus Fabio Scarpa Rosario Scivoli Roberto Bechere Maria A. Dettori Maria A. Montesu Vincenzo Marras Renato Lobrano Ciriaco Ligios Giantonella Puggioni |
| author_facet | Elisabetta Coradduzza Daria Sanna Angela M. Rocchigiani Davide Pintus Fabio Scarpa Rosario Scivoli Roberto Bechere Maria A. Dettori Maria A. Montesu Vincenzo Marras Renato Lobrano Ciriaco Ligios Giantonella Puggioni |
| author_sort | Elisabetta Coradduzza |
| collection | DOAJ |
| description | Orf virus (ORFV) represents the causative agent of contagious ecthyma, clinically characterized by mild papular and pustular to severe proliferative lesions, mainly occurring in sheep and goats. In order to provide hints on the evolutionary history of this virus, we carried out a study aimed to assess the genetic variation of ORFV in Sardinia that hosts a large affected small ruminant population. We also found a high worldwide mutational viral evolutionary rate, which resulted, in turn, higher than the rate we detected for the strains isolated in Sardinia. In addition, a well-supported genetic divergence was found between the viral strains isolated from sheep and those from goats, but no relevant connection was evidenced between the severity of lesions produced by ORFV and specific polymorphic patterns in the two species of hosts. Such a finding suggests that ORFV infection-related lesions are not necessarily linked to the expression of one of the three genes here analyzed and could rather be the effect of the expression of other genes or rather represents a multifactorial character. |
| first_indexed | 2024-03-10T11:43:12Z |
| format | Article |
| id | doaj.art-386e94c1f5794884a1bee55c9d8ea117 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T11:43:12Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-386e94c1f5794884a1bee55c9d8ea1172023-11-21T18:19:08ZengMDPI AGLife2075-17292021-05-0111541610.3390/life11050416Molecular Insights into the Genetic Variability of ORF Virus in a Mediterranean Region (Sardinia, Italy)Elisabetta Coradduzza0Daria Sanna1Angela M. Rocchigiani2Davide Pintus3Fabio Scarpa4Rosario Scivoli5Roberto Bechere6Maria A. Dettori7Maria A. Montesu8Vincenzo Marras9Renato Lobrano10Ciriaco Ligios11Giantonella Puggioni12Istituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, ItalyDipartimento di Scienze Biomediche, Università di Sassari, 07100 Sassari, ItalyIstituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, ItalyIstituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, ItalyDipartimento di Medicina Veterinaria, Università di Sassari, 07100 Sassari, ItalyIstituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, ItalyIstituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, ItalyIstituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, ItalyDipartimento di Scienze Mediche, Chirurgiche e Sperimentali, Università di Sassari, 07100 Sassari, ItalyDipartimento di Scienze Mediche, Chirurgiche e Sperimentali, Università di Sassari, 07100 Sassari, ItalyDipartimento di Scienze Mediche, Chirurgiche e Sperimentali, Università di Sassari, 07100 Sassari, ItalyIstituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, ItalyIstituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, ItalyOrf virus (ORFV) represents the causative agent of contagious ecthyma, clinically characterized by mild papular and pustular to severe proliferative lesions, mainly occurring in sheep and goats. In order to provide hints on the evolutionary history of this virus, we carried out a study aimed to assess the genetic variation of ORFV in Sardinia that hosts a large affected small ruminant population. We also found a high worldwide mutational viral evolutionary rate, which resulted, in turn, higher than the rate we detected for the strains isolated in Sardinia. In addition, a well-supported genetic divergence was found between the viral strains isolated from sheep and those from goats, but no relevant connection was evidenced between the severity of lesions produced by ORFV and specific polymorphic patterns in the two species of hosts. Such a finding suggests that ORFV infection-related lesions are not necessarily linked to the expression of one of the three genes here analyzed and could rather be the effect of the expression of other genes or rather represents a multifactorial character.https://www.mdpi.com/2075-1729/11/5/416contagious ecthymaphylogenetic analysismolecular datingVIRB2LO45 |
| spellingShingle | Elisabetta Coradduzza Daria Sanna Angela M. Rocchigiani Davide Pintus Fabio Scarpa Rosario Scivoli Roberto Bechere Maria A. Dettori Maria A. Montesu Vincenzo Marras Renato Lobrano Ciriaco Ligios Giantonella Puggioni Molecular Insights into the Genetic Variability of ORF Virus in a Mediterranean Region (Sardinia, Italy) Life contagious ecthyma phylogenetic analysis molecular dating VIR B2L O45 |
| title | Molecular Insights into the Genetic Variability of ORF Virus in a Mediterranean Region (Sardinia, Italy) |
| title_full | Molecular Insights into the Genetic Variability of ORF Virus in a Mediterranean Region (Sardinia, Italy) |
| title_fullStr | Molecular Insights into the Genetic Variability of ORF Virus in a Mediterranean Region (Sardinia, Italy) |
| title_full_unstemmed | Molecular Insights into the Genetic Variability of ORF Virus in a Mediterranean Region (Sardinia, Italy) |
| title_short | Molecular Insights into the Genetic Variability of ORF Virus in a Mediterranean Region (Sardinia, Italy) |
| title_sort | molecular insights into the genetic variability of orf virus in a mediterranean region sardinia italy |
| topic | contagious ecthyma phylogenetic analysis molecular dating VIR B2L O45 |
| url | https://www.mdpi.com/2075-1729/11/5/416 |
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