A Short Review on Cryoprotectants for 3D Protein Structure Analysis
The three-dimensional structure of protein is determined by analyzing diffraction data collected using X-ray beams. However, X-ray beam can damage protein crystals during data collection, lowering the quality of the crystal data. A way to prevent such damage is by treating protein crystals with cryo...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Crystals |
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| Online Access: | https://www.mdpi.com/2073-4352/12/2/138 |
| _version_ | 1797481472801636352 |
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| author | Kiyoung Jang Hyun Gi Kim Sandi Hnit San Hlaing MinSoung Kang Hui-Woog Choe Yong Ju Kim |
| author_facet | Kiyoung Jang Hyun Gi Kim Sandi Hnit San Hlaing MinSoung Kang Hui-Woog Choe Yong Ju Kim |
| author_sort | Kiyoung Jang |
| collection | DOAJ |
| description | The three-dimensional structure of protein is determined by analyzing diffraction data collected using X-ray beams. However, X-ray beam can damage protein crystals during data collection, lowering the quality of the crystal data. A way to prevent such damage is by treating protein crystals with cryoprotectants. The cryoprotectant stabilizes the protein crystal and prevents lowering the quality of the diffraction data. Many kinds of cryoprotectants are commercially available, and various treatment methods have also been reported. However, incorrect selection or treatment of such cryoprotectants may lead to deterioration of crystal diffraction data when using X-ray beams. |
| first_indexed | 2024-03-09T22:16:03Z |
| format | Article |
| id | doaj.art-3870efe1844f4a379c5bb0007847a218 |
| institution | Directory Open Access Journal |
| issn | 2073-4352 |
| language | English |
| last_indexed | 2024-03-09T22:16:03Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Crystals |
| spelling | doaj.art-3870efe1844f4a379c5bb0007847a2182023-11-23T19:23:31ZengMDPI AGCrystals2073-43522022-01-0112213810.3390/cryst12020138A Short Review on Cryoprotectants for 3D Protein Structure AnalysisKiyoung Jang0Hyun Gi Kim1Sandi Hnit San Hlaing2MinSoung Kang3Hui-Woog Choe4Yong Ju Kim5Department of Lifestyle Medicine, College of Environmental and Bioresource Sciences, Jeonbuk National University, Gobong-ro 79, Iksan 54596, KoreaDepartment of Lifestyle Medicine, College of Environmental and Bioresource Sciences, Jeonbuk National University, Gobong-ro 79, Iksan 54596, KoreaDepartment of Lifestyle Medicine, College of Environmental and Bioresource Sciences, Jeonbuk National University, Gobong-ro 79, Iksan 54596, KoreaDepartment of Lifestyle Medicine, College of Environmental and Bioresource Sciences, Jeonbuk National University, Gobong-ro 79, Iksan 54596, KoreaDepartment of Chemistry, College of Natural Science, Jeonbuk National University, Baekje-daero 567, Jeonju 54896, KoreaDepartment of Lifestyle Medicine, College of Environmental and Bioresource Sciences, Jeonbuk National University, Gobong-ro 79, Iksan 54596, KoreaThe three-dimensional structure of protein is determined by analyzing diffraction data collected using X-ray beams. However, X-ray beam can damage protein crystals during data collection, lowering the quality of the crystal data. A way to prevent such damage is by treating protein crystals with cryoprotectants. The cryoprotectant stabilizes the protein crystal and prevents lowering the quality of the diffraction data. Many kinds of cryoprotectants are commercially available, and various treatment methods have also been reported. However, incorrect selection or treatment of such cryoprotectants may lead to deterioration of crystal diffraction data when using X-ray beams.https://www.mdpi.com/2073-4352/12/2/138X-ray crystallographyprotein crystalscryoprotectant |
| spellingShingle | Kiyoung Jang Hyun Gi Kim Sandi Hnit San Hlaing MinSoung Kang Hui-Woog Choe Yong Ju Kim A Short Review on Cryoprotectants for 3D Protein Structure Analysis Crystals X-ray crystallography protein crystals cryoprotectant |
| title | A Short Review on Cryoprotectants for 3D Protein Structure Analysis |
| title_full | A Short Review on Cryoprotectants for 3D Protein Structure Analysis |
| title_fullStr | A Short Review on Cryoprotectants for 3D Protein Structure Analysis |
| title_full_unstemmed | A Short Review on Cryoprotectants for 3D Protein Structure Analysis |
| title_short | A Short Review on Cryoprotectants for 3D Protein Structure Analysis |
| title_sort | short review on cryoprotectants for 3d protein structure analysis |
| topic | X-ray crystallography protein crystals cryoprotectant |
| url | https://www.mdpi.com/2073-4352/12/2/138 |
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