Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix
Background: The unique lactation strategy of the tammar wallaby (Macropus eugeni) has been invaluable in evaluating the role of lactogenic hormones and the extracellular matrix (ECM) in the local control of mammary gland function. However molecular pathways through which hormones and ECM exert their...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-12-01
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| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580817300912 |
| _version_ | 1818020565692186624 |
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| author | Stephen S. Wanyonyi Amit Kumar Ryan Du Preez Christophe Lefevre Kevin R. Nicholas |
| author_facet | Stephen S. Wanyonyi Amit Kumar Ryan Du Preez Christophe Lefevre Kevin R. Nicholas |
| author_sort | Stephen S. Wanyonyi |
| collection | DOAJ |
| description | Background: The unique lactation strategy of the tammar wallaby (Macropus eugeni) has been invaluable in evaluating the role of lactogenic hormones and the extracellular matrix (ECM) in the local control of mammary gland function. However molecular pathways through which hormones and ECM exert their effect on wallaby mammary gland function remain unclear. This study undertakes transcriptome analysis of wallaby mammary epithelial cells (WallMEC) following treatment with mammary ECM from two distinct stages of lactation.
Methods: WallMEC from MID lactation mammary glands were cultured on ECM from MID or LATE lactation and treated for 5 days with 1 μg/ml cortisol, 1 μg/ml insulin, 0.2 µg/ml prolactin, 650 pg/ml triodothyronine and 1 pg/ml estradiol to induce lactation. WallMEC RNA from triplicate ECM treatments was used to perform RNAseq.
Results: ECM from MID and LATE lactation differentially regulated key genes in sugar and lipid metabolism. Seven pathways including galactose metabolism, lysosome, cell adhesion molecules (CAM), p53 signaling, the complement and coagulation and Nod-like receptor signaling pathways were only significantly responsive to ECM in the presence of hormones. The raw RNA-seq data for this project are available on the NCBI Gene Expression Omnibus (GEO) browser (accession number GSE81210).
Conclusions: A potential role of ECM in regulation of the caloric content of milk, among other functions including apoptosis, cell proliferation and differentiation has been identified.
General significance: This study has used a non-eutherian lactation model to demonstrate the synergy between ECM and hormones in the local regulation of mammary function. |
| first_indexed | 2024-04-14T08:07:53Z |
| format | Article |
| id | doaj.art-38714ace20c2453fafbf8b337d0923a3 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-04-14T08:07:53Z |
| publishDate | 2017-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-38714ace20c2453fafbf8b337d0923a32022-12-22T02:04:41ZengElsevierBiochemistry and Biophysics Reports2405-58082017-12-0112C12012810.1016/j.bbrep.2017.08.013Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrixStephen S. Wanyonyi0Amit Kumar1Ryan Du Preez2Christophe Lefevre3Kevin R. Nicholas4Institute for Agriculture and the Environment, University of Southern Queensland, West St., Toowoomba, QLD, AustraliaSchool of Medicine, Deakin University, VIC, AustraliaInstitute for Agriculture and the Environment, University of Southern Queensland, West St., Toowoomba, QLD, AustraliaSchool of Medicine, Deakin University, VIC, AustraliaSchool of Medicine, Deakin University, VIC, AustraliaBackground: The unique lactation strategy of the tammar wallaby (Macropus eugeni) has been invaluable in evaluating the role of lactogenic hormones and the extracellular matrix (ECM) in the local control of mammary gland function. However molecular pathways through which hormones and ECM exert their effect on wallaby mammary gland function remain unclear. This study undertakes transcriptome analysis of wallaby mammary epithelial cells (WallMEC) following treatment with mammary ECM from two distinct stages of lactation. Methods: WallMEC from MID lactation mammary glands were cultured on ECM from MID or LATE lactation and treated for 5 days with 1 μg/ml cortisol, 1 μg/ml insulin, 0.2 µg/ml prolactin, 650 pg/ml triodothyronine and 1 pg/ml estradiol to induce lactation. WallMEC RNA from triplicate ECM treatments was used to perform RNAseq. Results: ECM from MID and LATE lactation differentially regulated key genes in sugar and lipid metabolism. Seven pathways including galactose metabolism, lysosome, cell adhesion molecules (CAM), p53 signaling, the complement and coagulation and Nod-like receptor signaling pathways were only significantly responsive to ECM in the presence of hormones. The raw RNA-seq data for this project are available on the NCBI Gene Expression Omnibus (GEO) browser (accession number GSE81210). Conclusions: A potential role of ECM in regulation of the caloric content of milk, among other functions including apoptosis, cell proliferation and differentiation has been identified. General significance: This study has used a non-eutherian lactation model to demonstrate the synergy between ECM and hormones in the local regulation of mammary function.http://www.sciencedirect.com/science/article/pii/S2405580817300912Milk lipid composition |
| spellingShingle | Stephen S. Wanyonyi Amit Kumar Ryan Du Preez Christophe Lefevre Kevin R. Nicholas Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix Biochemistry and Biophysics Reports Milk lipid composition |
| title | Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix |
| title_full | Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix |
| title_fullStr | Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix |
| title_full_unstemmed | Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix |
| title_short | Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix |
| title_sort | transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix |
| topic | Milk lipid composition |
| url | http://www.sciencedirect.com/science/article/pii/S2405580817300912 |
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