Correlation analysis of circulating tumor cells and Claudin-4 in breast cancer
Objective: We aimed to explore the relationship between peripheral blood circulating tumor cells (CTCs) and the expression of Claudin-4 in patients with breast cancer, and further explore the potential impact on clinical prognosis and risk assessment.Methods: We classified and enumerated circulating...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Pathology and Oncology Research |
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| Online Access: | https://www.por-journal.com/articles/10.3389/pore.2023.1611224/full |
| _version_ | 1827290250993467392 |
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| author | Jie Chai Xiangli Liu Xinju Hu Chunfang Wang |
| author_facet | Jie Chai Xiangli Liu Xinju Hu Chunfang Wang |
| author_sort | Jie Chai |
| collection | DOAJ |
| description | Objective: We aimed to explore the relationship between peripheral blood circulating tumor cells (CTCs) and the expression of Claudin-4 in patients with breast cancer, and further explore the potential impact on clinical prognosis and risk assessment.Methods: We classified and enumerated circulating tumor cells in the blood of breast cancer patients by CTC-enriched in situ hybridization and the detection of Claudin-4 expression by immunohistochemistry. We carried out an analysis of the correlation between the two and the comparison of their impact on clinical parameters and prognosis.Results: There were 38 patients with a low expression of Claudin-4 and 27 patients with a high expression of Claudin-4. Compared with Claudin-4 low-expression patients, the number of CTCs was higher in patients with high Claudin-4 expression (11.7 vs. 7.4, p < 0.001). High Claudin-4 expression was associated with a lower count of epithelial CTCs (E-CTCs) (3.4 vs. 5.0, p = 0.033), higher counts of mesenchymal CTCs (M-CTC) (4.4 vs. 1.1, p < 0.001), and epithelial/mesenchymal CTCs (E/M-CTCs) (4.0 vs. 3.5, p = 0.021). The intensity of Claudin-4 was positively correlated with CTC (rs = 0.43, p = 0.001). Multivariate COX regression analysis showed that CTC counts (HR = 1.3, p < 0.001), Claudin-4 (HR = 4.6, p = 0.008), and Lymphatic metastasis (HR = 12.9, p = 0.001) were independent factors for poor prognosis. COX regression of CTC classification showed that epithelial/mesenchymal CTCs (E/M-CTC) (HR = 1.9, p = 0.001) and mesenchymal CTCs (M-CTC) (HR = 1.5, p = 0.001) were independent influencing factors of adverse reactions in breast cancer patients.Conclusion: The number of CTC in breast cancer is positively correlated with the expression of Claudin-4. High CTC counts and a high proportion of M-CTCs correlated with Claudin-4 expression. CTC counts and Claudin-4 expression were independent predictors of poor prognosis in breast cancer patients. |
| first_indexed | 2024-04-24T12:13:21Z |
| format | Article |
| id | doaj.art-387291c059354aa4ad748b5582fcf4ec |
| institution | Directory Open Access Journal |
| issn | 1532-2807 |
| language | English |
| last_indexed | 2024-04-24T12:13:21Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Pathology and Oncology Research |
| spelling | doaj.art-387291c059354aa4ad748b5582fcf4ec2024-04-08T09:40:38ZengFrontiers Media S.A.Pathology and Oncology Research1532-28072023-07-012910.3389/pore.2023.16112241611224Correlation analysis of circulating tumor cells and Claudin-4 in breast cancerJie Chai0Xiangli Liu1Xinju Hu2Chunfang Wang3Pathology Department, The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, ChinaPathology Department, The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, ChinaPathology Department, The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, ChinaBreast Surgery, The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, ChinaObjective: We aimed to explore the relationship between peripheral blood circulating tumor cells (CTCs) and the expression of Claudin-4 in patients with breast cancer, and further explore the potential impact on clinical prognosis and risk assessment.Methods: We classified and enumerated circulating tumor cells in the blood of breast cancer patients by CTC-enriched in situ hybridization and the detection of Claudin-4 expression by immunohistochemistry. We carried out an analysis of the correlation between the two and the comparison of their impact on clinical parameters and prognosis.Results: There were 38 patients with a low expression of Claudin-4 and 27 patients with a high expression of Claudin-4. Compared with Claudin-4 low-expression patients, the number of CTCs was higher in patients with high Claudin-4 expression (11.7 vs. 7.4, p < 0.001). High Claudin-4 expression was associated with a lower count of epithelial CTCs (E-CTCs) (3.4 vs. 5.0, p = 0.033), higher counts of mesenchymal CTCs (M-CTC) (4.4 vs. 1.1, p < 0.001), and epithelial/mesenchymal CTCs (E/M-CTCs) (4.0 vs. 3.5, p = 0.021). The intensity of Claudin-4 was positively correlated with CTC (rs = 0.43, p = 0.001). Multivariate COX regression analysis showed that CTC counts (HR = 1.3, p < 0.001), Claudin-4 (HR = 4.6, p = 0.008), and Lymphatic metastasis (HR = 12.9, p = 0.001) were independent factors for poor prognosis. COX regression of CTC classification showed that epithelial/mesenchymal CTCs (E/M-CTC) (HR = 1.9, p = 0.001) and mesenchymal CTCs (M-CTC) (HR = 1.5, p = 0.001) were independent influencing factors of adverse reactions in breast cancer patients.Conclusion: The number of CTC in breast cancer is positively correlated with the expression of Claudin-4. High CTC counts and a high proportion of M-CTCs correlated with Claudin-4 expression. CTC counts and Claudin-4 expression were independent predictors of poor prognosis in breast cancer patients.https://www.por-journal.com/articles/10.3389/pore.2023.1611224/fullbreast cancercirculating tumor cellstight junction proteinclaudin-4molecular subtype |
| spellingShingle | Jie Chai Xiangli Liu Xinju Hu Chunfang Wang Correlation analysis of circulating tumor cells and Claudin-4 in breast cancer Pathology and Oncology Research breast cancer circulating tumor cells tight junction protein claudin-4 molecular subtype |
| title | Correlation analysis of circulating tumor cells and Claudin-4 in breast cancer |
| title_full | Correlation analysis of circulating tumor cells and Claudin-4 in breast cancer |
| title_fullStr | Correlation analysis of circulating tumor cells and Claudin-4 in breast cancer |
| title_full_unstemmed | Correlation analysis of circulating tumor cells and Claudin-4 in breast cancer |
| title_short | Correlation analysis of circulating tumor cells and Claudin-4 in breast cancer |
| title_sort | correlation analysis of circulating tumor cells and claudin 4 in breast cancer |
| topic | breast cancer circulating tumor cells tight junction protein claudin-4 molecular subtype |
| url | https://www.por-journal.com/articles/10.3389/pore.2023.1611224/full |
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