Effects of high-frequency nanosecond pulses on prostate cancer cells
Abstract Electroporation with pulsed electric fields show a potential to be applied as an experimental focal therapy of tumors. Sub-microsecond regime of electric pulses displays unique electrophysical features operative in cells and membranes. Recently, MHz compression of nanosecond pulses electric...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-95180-7 |
| _version_ | 1818421053466083328 |
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| author | Aleksander Kiełbik Wojciech Szlasa Vitalij Novickij Anna Szewczyk Magdalena Maciejewska Jolanta Saczko Julita Kulbacka |
| author_facet | Aleksander Kiełbik Wojciech Szlasa Vitalij Novickij Anna Szewczyk Magdalena Maciejewska Jolanta Saczko Julita Kulbacka |
| author_sort | Aleksander Kiełbik |
| collection | DOAJ |
| description | Abstract Electroporation with pulsed electric fields show a potential to be applied as an experimental focal therapy of tumors. Sub-microsecond regime of electric pulses displays unique electrophysical features operative in cells and membranes. Recently, MHz compression of nanosecond pulses electric fields (nsPEFs) bursts proved to enhance the effectiveness of the therapy. High morbidity of prostate cancer (PCa) and risk of overtreatment associated with this malignancy call for new minimal-invasive treatment alternative. Herein we present the in vitro study for developing applications based on this new technology. In this study, we used flow cytometric analysis, cell viability assay, caspase activity analysis, wound healing assay, confocal microscopy study, and immunofluorescence to investigate the biological effect of high-frequency nsPEFs on PCa cells. Our results show that high-frequency nsPEFs induces the permeabilization and cell death of PCa cells. The cytotoxicity is significantly enhanced in MHz compression of pulses and with the presence of extracellular Ca2+. High-frequency nsPEFs trigger changes in PCa cells’ cytoskeleton and their mobility. The presented data show a therapeutic potential of high-frequency nsPEFs in a PCa setting. The sub-microsecond regime of pulses can potentially be applied in nanosecond electroporation protocols for PCa treatment. |
| first_indexed | 2024-12-14T13:04:15Z |
| format | Article |
| id | doaj.art-3872a8066eab4b84beacd7b0fd67424a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T13:04:15Z |
| publishDate | 2021-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3872a8066eab4b84beacd7b0fd67424a2022-12-21T23:00:21ZengNature PortfolioScientific Reports2045-23222021-08-0111111010.1038/s41598-021-95180-7Effects of high-frequency nanosecond pulses on prostate cancer cellsAleksander Kiełbik0Wojciech Szlasa1Vitalij Novickij2Anna Szewczyk3Magdalena Maciejewska4Jolanta Saczko5Julita Kulbacka6Medical University HospitalFaculty of Medicine, Wroclaw Medical UniversityInstitute of High Magnetic Fields, Vilnius Gediminas Technical UniversityDepartment of Molecular and Cellular Biology, Faculty of Pharmacy, Wroclaw Medical UniversityHirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of SciencesDepartment of Molecular and Cellular Biology, Faculty of Pharmacy, Wroclaw Medical UniversityDepartment of Molecular and Cellular Biology, Faculty of Pharmacy, Wroclaw Medical UniversityAbstract Electroporation with pulsed electric fields show a potential to be applied as an experimental focal therapy of tumors. Sub-microsecond regime of electric pulses displays unique electrophysical features operative in cells and membranes. Recently, MHz compression of nanosecond pulses electric fields (nsPEFs) bursts proved to enhance the effectiveness of the therapy. High morbidity of prostate cancer (PCa) and risk of overtreatment associated with this malignancy call for new minimal-invasive treatment alternative. Herein we present the in vitro study for developing applications based on this new technology. In this study, we used flow cytometric analysis, cell viability assay, caspase activity analysis, wound healing assay, confocal microscopy study, and immunofluorescence to investigate the biological effect of high-frequency nsPEFs on PCa cells. Our results show that high-frequency nsPEFs induces the permeabilization and cell death of PCa cells. The cytotoxicity is significantly enhanced in MHz compression of pulses and with the presence of extracellular Ca2+. High-frequency nsPEFs trigger changes in PCa cells’ cytoskeleton and their mobility. The presented data show a therapeutic potential of high-frequency nsPEFs in a PCa setting. The sub-microsecond regime of pulses can potentially be applied in nanosecond electroporation protocols for PCa treatment.https://doi.org/10.1038/s41598-021-95180-7 |
| spellingShingle | Aleksander Kiełbik Wojciech Szlasa Vitalij Novickij Anna Szewczyk Magdalena Maciejewska Jolanta Saczko Julita Kulbacka Effects of high-frequency nanosecond pulses on prostate cancer cells Scientific Reports |
| title | Effects of high-frequency nanosecond pulses on prostate cancer cells |
| title_full | Effects of high-frequency nanosecond pulses on prostate cancer cells |
| title_fullStr | Effects of high-frequency nanosecond pulses on prostate cancer cells |
| title_full_unstemmed | Effects of high-frequency nanosecond pulses on prostate cancer cells |
| title_short | Effects of high-frequency nanosecond pulses on prostate cancer cells |
| title_sort | effects of high frequency nanosecond pulses on prostate cancer cells |
| url | https://doi.org/10.1038/s41598-021-95180-7 |
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