ТiО(2) hepatotoxicity under long-term administration to rats
Titanium dioxide (ТіО2) powder which is used as a white dye was considered to be an inert material for a long time despite its accumulation in liver tissues after penetration into organism. The aim of the study was to estimate biochemical markers of liver functioning in blood plasma and ATPase ac...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2020-08-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2020/09/Tsymbalyuk_4_20.pdf |
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| author | O. V. Tsymbalyuk S. P. Veselsky A. M. Naumenko T. L. Davydovska I. S. Voiteshenko I. I. Сhyzh V. A. Skryshevsky |
| author_facet | O. V. Tsymbalyuk S. P. Veselsky A. M. Naumenko T. L. Davydovska I. S. Voiteshenko I. I. Сhyzh V. A. Skryshevsky |
| author_sort | O. V. Tsymbalyuk |
| collection | DOAJ |
| description | Titanium dioxide (ТіО2) powder which is used as a white dye was considered to be an inert material for a long time despite its accumulation in liver tissues after penetration into organism. The aim of the study was to estimate biochemical markers of liver functioning in blood plasma and ATPase activity of erythrocyte plasma membrane under the oral administration of ТіО2 nanoparticles suspension (0.1 mg/kg, daily) to Wistar rats for 30 and 100 days. A significant increase of alanine aminotransferase and aspartate aminotransferase activity as well as of direct, indirect and bound bilirubin content, a decrease of connjugated (taurocholic, taurochenodeoxycholic, taurodeoxycholic, glycocholic, glycochenodeoxycholic, and glycodeoxycholic) and free (glycodeoxycholic and deoxycholic) bile acids concentration with concomitant increase of free cholic acid concentration in blood plasma of rats under ТіО2 administration were revealed, indicating a significant impairment of pigment exchange in the liver of rats. Under ТіО2 administration a substantial inhibition of erythrocyte plasma membrane Мg2+-dependent ouabain-sensitive Na+,K+-ATPase and ouabain-insensitive ATPase was observed. These results presume the disturbance of transplasmalema ion-transporting processes and cells ionic homeostasis induced by ТіО2. |
| first_indexed | 2024-03-11T13:49:57Z |
| format | Article |
| id | doaj.art-3874b3b8bcaa40cbb535967264c7ed37 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-11T13:49:57Z |
| publishDate | 2020-08-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-3874b3b8bcaa40cbb535967264c7ed372023-11-02T09:08:28ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032020-08-01924455410.15407/ubj92.04.045ТiО(2) hepatotoxicity under long-term administration to ratsO. V. Tsymbalyuk 0https://orcid.org/0000-0002-4524-7627S. P. Veselsky 1https://orcid.org/0000-0001-9971-0333A. M. Naumenko 2T. L. Davydovska3I. S. Voiteshenko4https://orcid.org/0000-0003-2434-9218I. I. Сhyzh5V. A. Skryshevsky6https://orcid.org/0000-0003-0249-5556Institute of High Technologies, Taras Shevchenko National University of Kyiv, UkraineInstitute of High Technologies, Taras Shevchenko National University of Kyiv, UkraineInstitute of High Technologies, Taras Shevchenko National University of Kyiv, UkraineInstitute of High Technologies, Taras Shevchenko National University of Kyiv, UkraineInstitute of High Technologies, Taras Shevchenko National University of Kyiv, UkraineInstitute of High Technologies, Taras Shevchenko National University of Kyiv, UkraineInstitute of High Technologies, Taras Shevchenko National University of Kyiv, UkraineTitanium dioxide (ТіО2) powder which is used as a white dye was considered to be an inert material for a long time despite its accumulation in liver tissues after penetration into organism. The aim of the study was to estimate biochemical markers of liver functioning in blood plasma and ATPase activity of erythrocyte plasma membrane under the oral administration of ТіО2 nanoparticles suspension (0.1 mg/kg, daily) to Wistar rats for 30 and 100 days. A significant increase of alanine aminotransferase and aspartate aminotransferase activity as well as of direct, indirect and bound bilirubin content, a decrease of connjugated (taurocholic, taurochenodeoxycholic, taurodeoxycholic, glycocholic, glycochenodeoxycholic, and glycodeoxycholic) and free (glycodeoxycholic and deoxycholic) bile acids concentration with concomitant increase of free cholic acid concentration in blood plasma of rats under ТіО2 administration were revealed, indicating a significant impairment of pigment exchange in the liver of rats. Under ТіО2 administration a substantial inhibition of erythrocyte plasma membrane Мg2+-dependent ouabain-sensitive Na+,K+-ATPase and ouabain-insensitive ATPase was observed. These results presume the disturbance of transplasmalema ion-transporting processes and cells ionic homeostasis induced by ТіО2.http://ukrbiochemjournal.org/wp-content/uploads/2020/09/Tsymbalyuk_4_20.pdfalanine aminotransferaseaspartate aminotransferasebile acidsbilirubinplasma membrane atpase activitiestio2 nanoparticles |
| spellingShingle | O. V. Tsymbalyuk S. P. Veselsky A. M. Naumenko T. L. Davydovska I. S. Voiteshenko I. I. Сhyzh V. A. Skryshevsky ТiО(2) hepatotoxicity under long-term administration to rats The Ukrainian Biochemical Journal alanine aminotransferase aspartate aminotransferase bile acids bilirubin plasma membrane atpase activities tio2 nanoparticles |
| title | ТiО(2) hepatotoxicity under long-term administration to rats |
| title_full | ТiО(2) hepatotoxicity under long-term administration to rats |
| title_fullStr | ТiО(2) hepatotoxicity under long-term administration to rats |
| title_full_unstemmed | ТiО(2) hepatotoxicity under long-term administration to rats |
| title_short | ТiО(2) hepatotoxicity under long-term administration to rats |
| title_sort | тiо 2 hepatotoxicity under long term administration to rats |
| topic | alanine aminotransferase aspartate aminotransferase bile acids bilirubin plasma membrane atpase activities tio2 nanoparticles |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2020/09/Tsymbalyuk_4_20.pdf |
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