Gastroprotective Mechanisms of the Monoterpene 1,8-Cineole (Eucalyptol).
Recently, our research group identified and reported 1,8-cineole (CIN), a monoterpene that naturally occur in many aromatic plants, as one of the major constituent of the essential oil from leaves of Hyptis martiusii (EOHM), as well as characterized the gastroprotective action of this oil. The aim o...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0134558 |
| _version_ | 1818727326928601088 |
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| author | Germana Freire Rocha Caldas Alisson Rodrigo da Silva Oliveira Alice Valença Araújo Simone Sette Lopes Lafayette Giwellington Silva Albuquerque Jacinto da Costa Silva-Neto João Henrique Costa-Silva Fabiano Ferreira José Galberto Martins da Costa Almir Gonçalves Wanderley |
| author_facet | Germana Freire Rocha Caldas Alisson Rodrigo da Silva Oliveira Alice Valença Araújo Simone Sette Lopes Lafayette Giwellington Silva Albuquerque Jacinto da Costa Silva-Neto João Henrique Costa-Silva Fabiano Ferreira José Galberto Martins da Costa Almir Gonçalves Wanderley |
| author_sort | Germana Freire Rocha Caldas |
| collection | DOAJ |
| description | Recently, our research group identified and reported 1,8-cineole (CIN), a monoterpene that naturally occur in many aromatic plants, as one of the major constituent of the essential oil from leaves of Hyptis martiusii (EOHM), as well as characterized the gastroprotective action of this oil. The aim of this study was to investigate the mechanisms of action involved in the antiulcer and healing activity of CIN, in order to confirm its correlation with the gastroprotective effect of EOHM. Wistar rats were exposed to different protocols (acute ulceration, gastrointestinal motility and antisecretory activity). In addition, were determinated the involvement of nitric oxide and sulphydryl groups; the levels of gastric mucus, lipid peroxidation, sulphydryl groups and myeloperoxidase activity. The healing ability was evaluated by acetic acid-induced chronic ulcer and histological and immunohistochemical analysis (PCNA, Ki-67 and BrdU). The treatment with CIN inhibited ethanol-, ethanol/HCl- and indomethacin-induced gastric lesions. The highest doses of CIN inhibited gastric emptying, but did not affect intestinal transit. CIN (100 mg/kg) reduced the volume of basal but not stimulated acid secretion. CIN increased levels of mucus (89.3%), prevented depletion of -SH groups (62.6%) and reduced the level of lipid peroxidation (55.3%) and myeloperoxidase activity (59.4%) in the gastric mucosa. In chronic ulcer model, CIN reduced in 43.1% the gastric area lesion, promoted significant regeneration and restoration of the levels of mucus in glandular cells as confirmed by histological analysis; and promoted increase in cell proliferation as evidenced by reactivity for PCNA, Ki-67 and BrdU. This findings demonstrate the role of 1,8-cineole as an important ulcer healing agent and indicate the involvement of antioxidant and cytoprotective mechanisms in the gastroprotective effect of compound. This study also provides evidence that 1,8-cineole is related to the gastroprotective effect of the essential oil of Hyptis martiusii. |
| first_indexed | 2024-12-17T22:12:20Z |
| format | Article |
| id | doaj.art-3877f5b8919e466f93083a31955fb3c1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T22:12:20Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3877f5b8919e466f93083a31955fb3c12022-12-21T21:30:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01108e013455810.1371/journal.pone.0134558Gastroprotective Mechanisms of the Monoterpene 1,8-Cineole (Eucalyptol).Germana Freire Rocha CaldasAlisson Rodrigo da Silva OliveiraAlice Valença AraújoSimone Sette Lopes LafayetteGiwellington Silva AlbuquerqueJacinto da Costa Silva-NetoJoão Henrique Costa-SilvaFabiano FerreiraJosé Galberto Martins da CostaAlmir Gonçalves WanderleyRecently, our research group identified and reported 1,8-cineole (CIN), a monoterpene that naturally occur in many aromatic plants, as one of the major constituent of the essential oil from leaves of Hyptis martiusii (EOHM), as well as characterized the gastroprotective action of this oil. The aim of this study was to investigate the mechanisms of action involved in the antiulcer and healing activity of CIN, in order to confirm its correlation with the gastroprotective effect of EOHM. Wistar rats were exposed to different protocols (acute ulceration, gastrointestinal motility and antisecretory activity). In addition, were determinated the involvement of nitric oxide and sulphydryl groups; the levels of gastric mucus, lipid peroxidation, sulphydryl groups and myeloperoxidase activity. The healing ability was evaluated by acetic acid-induced chronic ulcer and histological and immunohistochemical analysis (PCNA, Ki-67 and BrdU). The treatment with CIN inhibited ethanol-, ethanol/HCl- and indomethacin-induced gastric lesions. The highest doses of CIN inhibited gastric emptying, but did not affect intestinal transit. CIN (100 mg/kg) reduced the volume of basal but not stimulated acid secretion. CIN increased levels of mucus (89.3%), prevented depletion of -SH groups (62.6%) and reduced the level of lipid peroxidation (55.3%) and myeloperoxidase activity (59.4%) in the gastric mucosa. In chronic ulcer model, CIN reduced in 43.1% the gastric area lesion, promoted significant regeneration and restoration of the levels of mucus in glandular cells as confirmed by histological analysis; and promoted increase in cell proliferation as evidenced by reactivity for PCNA, Ki-67 and BrdU. This findings demonstrate the role of 1,8-cineole as an important ulcer healing agent and indicate the involvement of antioxidant and cytoprotective mechanisms in the gastroprotective effect of compound. This study also provides evidence that 1,8-cineole is related to the gastroprotective effect of the essential oil of Hyptis martiusii.https://doi.org/10.1371/journal.pone.0134558 |
| spellingShingle | Germana Freire Rocha Caldas Alisson Rodrigo da Silva Oliveira Alice Valença Araújo Simone Sette Lopes Lafayette Giwellington Silva Albuquerque Jacinto da Costa Silva-Neto João Henrique Costa-Silva Fabiano Ferreira José Galberto Martins da Costa Almir Gonçalves Wanderley Gastroprotective Mechanisms of the Monoterpene 1,8-Cineole (Eucalyptol). PLoS ONE |
| title | Gastroprotective Mechanisms of the Monoterpene 1,8-Cineole (Eucalyptol). |
| title_full | Gastroprotective Mechanisms of the Monoterpene 1,8-Cineole (Eucalyptol). |
| title_fullStr | Gastroprotective Mechanisms of the Monoterpene 1,8-Cineole (Eucalyptol). |
| title_full_unstemmed | Gastroprotective Mechanisms of the Monoterpene 1,8-Cineole (Eucalyptol). |
| title_short | Gastroprotective Mechanisms of the Monoterpene 1,8-Cineole (Eucalyptol). |
| title_sort | gastroprotective mechanisms of the monoterpene 1 8 cineole eucalyptol |
| url | https://doi.org/10.1371/journal.pone.0134558 |
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