Progesterone significantly enhances the mobility of boar spermatozoa
Progesterone released from the cumulus cells of the oocyte causes a number of physiological responses in human sperm cells including hyperactivation, acrosome reaction and chemotaxis. We employed a validated sperm mobility assay, which involves measuring the ability of sperm to penetrate an inert ce...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2013-12-01
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| Series: | BioDiscovery |
| Subjects: | |
| Online Access: | https://biodiscovery.pensoft.net/article/8955/download/pdf/ |
| _version_ | 1818158364720365568 |
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| author | Johan M. Campbell Anne L. Savage Oladipo Madamidola Kshitij Tamhane Renalyn Soriano Ashok K. Adya Sean G. Brown |
| author_facet | Johan M. Campbell Anne L. Savage Oladipo Madamidola Kshitij Tamhane Renalyn Soriano Ashok K. Adya Sean G. Brown |
| author_sort | Johan M. Campbell |
| collection | DOAJ |
| description | Progesterone released from the cumulus cells of the oocyte causes a number of physiological responses in human sperm cells including hyperactivation, acrosome reaction and chemotaxis. We employed a validated sperm mobility assay, which involves measuring the ability of sperm to penetrate an inert cell separation solution over time, to assess the ability of progesterone to enhance the mobility of boar spermatozoa. Cells maximally penetrate the solution over 50 minutes. 100nM progesterone significantly (P = 0.01) increased the mobility of non-capacitated sperm cells causing a doubling in the rate at which the cells penetrated through the cell separation solution (control half maximal penetration rate [Km] = 18.0±2.2; +100nM progesterone Km = 8.8±0.8min). Similarly, capacitated cells penetrated at a rate (Km = 19.2±3.0 min) not significantly different from non-capacitated cells and 100nM progesterone also significantly increased the rate of penetration of capacitated cells (Km = 9.5±1.0 min, P<0.05). The T-type voltage gated calcium channel blocker mibefradil (30mM) significantly inhibited both the control and progesterone enhanced mobility in non-capacitated and capacitated sperm. Only capacitated cells showed a significant increase in the acrosome reaction in response to 100nM progesterone (control non-reacted = 75±4%, +100nM progesterone non-reacted = 47±10%). Western blot analysis confirmed that there was an increase in the total protein tyrosine phosphorylation levels in capacitated cells. In conclusion, we have demonstrated that 100nM progesterone accelerates the mobility of boar sperm cells through an inert cell separation solution in an extracellular calcium dependent manner. |
| first_indexed | 2024-12-11T15:28:55Z |
| format | Article |
| id | doaj.art-388125e8d80d48cc9a416375b97b96fe |
| institution | Directory Open Access Journal |
| issn | 2050-2966 |
| language | English |
| last_indexed | 2024-12-11T15:28:55Z |
| publishDate | 2013-12-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | BioDiscovery |
| spelling | doaj.art-388125e8d80d48cc9a416375b97b96fe2022-12-22T01:00:07ZengPensoft PublishersBioDiscovery2050-29662013-12-0191710.7750/BioDiscovery.2013.9.58955Progesterone significantly enhances the mobility of boar spermatozoaJohan M. CampbellAnne L. SavageOladipo MadamidolaKshitij TamhaneRenalyn SorianoAshok K. AdyaSean G. BrownProgesterone released from the cumulus cells of the oocyte causes a number of physiological responses in human sperm cells including hyperactivation, acrosome reaction and chemotaxis. We employed a validated sperm mobility assay, which involves measuring the ability of sperm to penetrate an inert cell separation solution over time, to assess the ability of progesterone to enhance the mobility of boar spermatozoa. Cells maximally penetrate the solution over 50 minutes. 100nM progesterone significantly (P = 0.01) increased the mobility of non-capacitated sperm cells causing a doubling in the rate at which the cells penetrated through the cell separation solution (control half maximal penetration rate [Km] = 18.0±2.2; +100nM progesterone Km = 8.8±0.8min). Similarly, capacitated cells penetrated at a rate (Km = 19.2±3.0 min) not significantly different from non-capacitated cells and 100nM progesterone also significantly increased the rate of penetration of capacitated cells (Km = 9.5±1.0 min, P<0.05). The T-type voltage gated calcium channel blocker mibefradil (30mM) significantly inhibited both the control and progesterone enhanced mobility in non-capacitated and capacitated sperm. Only capacitated cells showed a significant increase in the acrosome reaction in response to 100nM progesterone (control non-reacted = 75±4%, +100nM progesterone non-reacted = 47±10%). Western blot analysis confirmed that there was an increase in the total protein tyrosine phosphorylation levels in capacitated cells. In conclusion, we have demonstrated that 100nM progesterone accelerates the mobility of boar sperm cells through an inert cell separation solution in an extracellular calcium dependent manner.https://biodiscovery.pensoft.net/article/8955/download/pdf/spermprogesteronemobilityhyperactivationbo |
| spellingShingle | Johan M. Campbell Anne L. Savage Oladipo Madamidola Kshitij Tamhane Renalyn Soriano Ashok K. Adya Sean G. Brown Progesterone significantly enhances the mobility of boar spermatozoa BioDiscovery sperm progesterone mobility hyperactivation bo |
| title | Progesterone significantly enhances the mobility of boar spermatozoa |
| title_full | Progesterone significantly enhances the mobility of boar spermatozoa |
| title_fullStr | Progesterone significantly enhances the mobility of boar spermatozoa |
| title_full_unstemmed | Progesterone significantly enhances the mobility of boar spermatozoa |
| title_short | Progesterone significantly enhances the mobility of boar spermatozoa |
| title_sort | progesterone significantly enhances the mobility of boar spermatozoa |
| topic | sperm progesterone mobility hyperactivation bo |
| url | https://biodiscovery.pensoft.net/article/8955/download/pdf/ |
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