Evaluation of Marine Microalga Diacronema vlkianum Biomass Fatty Acid Assimilation in Wistar Rats
Diacronema vlkianum is a marine microalgae for which supposed health promoting effects have been claimed based on its phytochemical composition. The potential use of its biomass as health ingredient, including detox-shakes, and the lack of bioavailability studies were the main concerns. In order to...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/22/7/1097 |
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| author | Cristina de Mello-Sampayo Angela Paterna Ambra Polizzi Diana Duarte Irineu Batista Rui Pinto Patrícia Gonçalves Anabela Raymundo Ana P. Batista Luísa Gouveia Beatriz Silva-Lima Narcisa M. Bandarra |
| author_facet | Cristina de Mello-Sampayo Angela Paterna Ambra Polizzi Diana Duarte Irineu Batista Rui Pinto Patrícia Gonçalves Anabela Raymundo Ana P. Batista Luísa Gouveia Beatriz Silva-Lima Narcisa M. Bandarra |
| author_sort | Cristina de Mello-Sampayo |
| collection | DOAJ |
| description | Diacronema vlkianum is a marine microalgae for which supposed health promoting effects have been claimed based on its phytochemical composition. The potential use of its biomass as health ingredient, including detox-shakes, and the lack of bioavailability studies were the main concerns. In order to evaluate the microalgae-biomass assimilation and its health-benefits, single-dose (CD1-mice) studies were followed by 66-days repeated-dose study in Wistar rats with the highest tested single-dose of microalgae equivalent to 101 mg/kg eicosapentaenoic acid + docosahexaenoic acid (EPA+DHA). Microalgae-supplementation modulated EPA and docosapentaenoic acid enrichment at arachidonic acid content expenditure in erythrocytes and liver, while increasing EPA content of heart and adipose tissues of rats. Those fatty acid (FA) changes confirmed the D. vlkianum-biomass FA assimilation. The principal component analyses discriminated brain from other tissues, which formed two other groups (erythrocytes, liver, and heart separated from kidney and adipose tissues), pointing to a distinct signature of FA deposition for the brain and for the other organs. The improved serum lipid profile, omega-3 index and erythrocyte plasticity support the cardiovascular benefits of D. vlkianum. These results bolster the potential of D. vlkianum-biomass to become a “heart-healthy” food supplement providing a safe and renewable source of bioavailable omega-3 FA. |
| first_indexed | 2024-12-12T01:44:17Z |
| format | Article |
| id | doaj.art-38859c49c0b74523adbd5c1a52445525 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-12T01:44:17Z |
| publishDate | 2017-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-38859c49c0b74523adbd5c1a524455252022-12-22T00:42:37ZengMDPI AGMolecules1420-30492017-07-01227109710.3390/molecules22071097molecules22071097Evaluation of Marine Microalga Diacronema vlkianum Biomass Fatty Acid Assimilation in Wistar RatsCristina de Mello-Sampayo0Angela Paterna1Ambra Polizzi2Diana Duarte3Irineu Batista4Rui Pinto5Patrícia Gonçalves6Anabela Raymundo7Ana P. Batista8Luísa Gouveia9Beatriz Silva-Lima10Narcisa M. Bandarra11Department of Pharmacological Sciences, iMed.ULisboa, Faculdade de Farmácia, Universidade de Lisboa, Lisbon 1649-003, PortugalDepartment of Pharmacological Sciences, iMed.ULisboa, Faculdade de Farmácia, Universidade de Lisboa, Lisbon 1649-003, PortugalDepartment of Pharmacological Sciences, iMed.ULisboa, Faculdade de Farmácia, Universidade de Lisboa, Lisbon 1649-003, PortugalDivision of Aquaculture and Upgrading, Portuguese Institute for the Sea and Atmosphere (IPMA, IP), Rua Alfredo Magalhães Ramalho, Lisbon 1495-006, PortugalDivision of Aquaculture and Upgrading, Portuguese Institute for the Sea and Atmosphere (IPMA, IP), Rua Alfredo Magalhães Ramalho, Lisbon 1495-006, PortugalDepartment of Pharmacological Sciences, iMed.ULisboa, Faculdade de Farmácia, Universidade de Lisboa, Lisbon 1649-003, PortugalDivision of Modelling and Marine Resources, Portuguese Institute for the Sea and Atmosphere (IPMA, IP), Rua Alfredo Magalhães Ramalho, Lisbon 1495-006, PortugalLEAF (Linking Landscape Environment Agriculture and Food), Research Center. Instituto Superior de Agronomia, Universidade de Lisboa. Tapada da Ajuda, 1349-017 Lisbon, PortugalLEAF (Linking Landscape Environment Agriculture and Food), Research Center. Instituto Superior de Agronomia, Universidade de Lisboa. Tapada da Ajuda, 1349-017 Lisbon, PortugalLNEG_Bioenergy Unit, Estrada do Paço do Lumiar, 22, 1649-038 Lisbon, PortugalDepartment of Pharmacological Sciences, iMed.ULisboa, Faculdade de Farmácia, Universidade de Lisboa, Lisbon 1649-003, PortugalDivision of Aquaculture and Upgrading, Portuguese Institute for the Sea and Atmosphere (IPMA, IP), Rua Alfredo Magalhães Ramalho, Lisbon 1495-006, PortugalDiacronema vlkianum is a marine microalgae for which supposed health promoting effects have been claimed based on its phytochemical composition. The potential use of its biomass as health ingredient, including detox-shakes, and the lack of bioavailability studies were the main concerns. In order to evaluate the microalgae-biomass assimilation and its health-benefits, single-dose (CD1-mice) studies were followed by 66-days repeated-dose study in Wistar rats with the highest tested single-dose of microalgae equivalent to 101 mg/kg eicosapentaenoic acid + docosahexaenoic acid (EPA+DHA). Microalgae-supplementation modulated EPA and docosapentaenoic acid enrichment at arachidonic acid content expenditure in erythrocytes and liver, while increasing EPA content of heart and adipose tissues of rats. Those fatty acid (FA) changes confirmed the D. vlkianum-biomass FA assimilation. The principal component analyses discriminated brain from other tissues, which formed two other groups (erythrocytes, liver, and heart separated from kidney and adipose tissues), pointing to a distinct signature of FA deposition for the brain and for the other organs. The improved serum lipid profile, omega-3 index and erythrocyte plasticity support the cardiovascular benefits of D. vlkianum. These results bolster the potential of D. vlkianum-biomass to become a “heart-healthy” food supplement providing a safe and renewable source of bioavailable omega-3 FA.https://www.mdpi.com/1420-3049/22/7/1097Diacronema vlkianum supplementationmicroalgae-biomass bioavailabilityEPADHAomega-3 indexcardiovascular benefitstissues fatty acid profile |
| spellingShingle | Cristina de Mello-Sampayo Angela Paterna Ambra Polizzi Diana Duarte Irineu Batista Rui Pinto Patrícia Gonçalves Anabela Raymundo Ana P. Batista Luísa Gouveia Beatriz Silva-Lima Narcisa M. Bandarra Evaluation of Marine Microalga Diacronema vlkianum Biomass Fatty Acid Assimilation in Wistar Rats Molecules Diacronema vlkianum supplementation microalgae-biomass bioavailability EPA DHA omega-3 index cardiovascular benefits tissues fatty acid profile |
| title | Evaluation of Marine Microalga Diacronema vlkianum Biomass Fatty Acid Assimilation in Wistar Rats |
| title_full | Evaluation of Marine Microalga Diacronema vlkianum Biomass Fatty Acid Assimilation in Wistar Rats |
| title_fullStr | Evaluation of Marine Microalga Diacronema vlkianum Biomass Fatty Acid Assimilation in Wistar Rats |
| title_full_unstemmed | Evaluation of Marine Microalga Diacronema vlkianum Biomass Fatty Acid Assimilation in Wistar Rats |
| title_short | Evaluation of Marine Microalga Diacronema vlkianum Biomass Fatty Acid Assimilation in Wistar Rats |
| title_sort | evaluation of marine microalga diacronema vlkianum biomass fatty acid assimilation in wistar rats |
| topic | Diacronema vlkianum supplementation microalgae-biomass bioavailability EPA DHA omega-3 index cardiovascular benefits tissues fatty acid profile |
| url | https://www.mdpi.com/1420-3049/22/7/1097 |
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