Dietary Selenium-Rich <i>Lactobacillus plantarum</i> Alleviates Cadmium-Induced Oxidative Stress and Inflammation in <i>Bulatmai barbel Luciobarbus capito</i>
Cadmium (Cd) poses a great threat to the breeding of aquatic economic animals. The present study aimed to explain the antagonistic effects of selenium-enriched <i>Lactobacillus plantarum</i> (SL) on Cd toxicity through the expression of oxidative and inflammatory factors. A total of 225...
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MDPI AG
2023-02-01
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| Online Access: | https://www.mdpi.com/2410-3888/8/3/136 |
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| author | Qing Zhang Xinchi Shang Longwu Geng Xinghua Che Haijun Wei Shizhan Tang Wei Xu |
| author_facet | Qing Zhang Xinchi Shang Longwu Geng Xinghua Che Haijun Wei Shizhan Tang Wei Xu |
| author_sort | Qing Zhang |
| collection | DOAJ |
| description | Cadmium (Cd) poses a great threat to the breeding of aquatic economic animals. The present study aimed to explain the antagonistic effects of selenium-enriched <i>Lactobacillus plantarum</i> (SL) on Cd toxicity through the expression of oxidative and inflammatory factors. A total of 225 <i>Bulatmai barbel Luciobarbus capito</i> (<i>L. capito</i>) were divided into 3 groups, namely, the control group, the Cd group (Cd, 0.05 mg·L<sup>−1</sup>), and the SL + Cd group (Cd, 0.05 mg·L<sup>−1</sup>; Nano Se, 5 mg·kg<sup>−1</sup>; <i>L. plantarum</i>, 10<sup>5</sup> CFU·g<sup>−1</sup>). The experiment lasted for 28 d, Sampling at 14 and 28 d, respectively. The results showed that Cd exposure caused obvious pathological damage to the liver and kidney, and the serum parameter ALT increased significantly (<i>p</i> < 0.05). In the Cd group, the concentration of Cd in the kidney was significantly increased (<i>p</i> < 0.05), and the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in the kidney and gill were significantly decreased; malonaldehyde (MDA) increased significantly (<i>p</i> < 0.05) Then the mRNA expression levels in the kidney and liver were measured. Cd exposure significantly decreased the mRNA expressions of <i>SOD</i>, <i>CAT</i>, <i>GSH-Px</i>, <i>HO-1</i>, <i>I-κBα,</i> and <i>Nrf2</i> (<i>p</i> < 0.05). The Cd-treated group showed significantly increased <i>NF-kBp65</i>, <i>TGF-β</i>, <i>IL-8</i>, <i>IL-1</i>, <i>Keap1,</i> and <i>TNF-α</i> expression levels. SL significantly alleviated the changes in the above indicators. The results of this study suggest that SL can trigger the Nrf2 signalling pathway and NF-kB signalling pathway to alleviate Cd toxicity. SL might be a potential drug for the treatment of Cd poisoning. |
| first_indexed | 2024-03-11T06:32:48Z |
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| issn | 2410-3888 |
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| last_indexed | 2024-03-11T06:32:48Z |
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| spelling | doaj.art-3887486120aa4f00bf9f2804243d50f92023-11-17T11:04:06ZengMDPI AGFishes2410-38882023-02-018313610.3390/fishes8030136Dietary Selenium-Rich <i>Lactobacillus plantarum</i> Alleviates Cadmium-Induced Oxidative Stress and Inflammation in <i>Bulatmai barbel Luciobarbus capito</i>Qing Zhang0Xinchi Shang1Longwu Geng2Xinghua Che3Haijun Wei4Shizhan Tang5Wei Xu6Key Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of Heilongjiang Province, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, ChinaKey Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of Heilongjiang Province, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, ChinaKey Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of Heilongjiang Province, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, ChinaKey Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of Heilongjiang Province, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, ChinaKey Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of Heilongjiang Province, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, ChinaKey Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of Heilongjiang Province, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, ChinaKey Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of Heilongjiang Province, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, ChinaCadmium (Cd) poses a great threat to the breeding of aquatic economic animals. The present study aimed to explain the antagonistic effects of selenium-enriched <i>Lactobacillus plantarum</i> (SL) on Cd toxicity through the expression of oxidative and inflammatory factors. A total of 225 <i>Bulatmai barbel Luciobarbus capito</i> (<i>L. capito</i>) were divided into 3 groups, namely, the control group, the Cd group (Cd, 0.05 mg·L<sup>−1</sup>), and the SL + Cd group (Cd, 0.05 mg·L<sup>−1</sup>; Nano Se, 5 mg·kg<sup>−1</sup>; <i>L. plantarum</i>, 10<sup>5</sup> CFU·g<sup>−1</sup>). The experiment lasted for 28 d, Sampling at 14 and 28 d, respectively. The results showed that Cd exposure caused obvious pathological damage to the liver and kidney, and the serum parameter ALT increased significantly (<i>p</i> < 0.05). In the Cd group, the concentration of Cd in the kidney was significantly increased (<i>p</i> < 0.05), and the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in the kidney and gill were significantly decreased; malonaldehyde (MDA) increased significantly (<i>p</i> < 0.05) Then the mRNA expression levels in the kidney and liver were measured. Cd exposure significantly decreased the mRNA expressions of <i>SOD</i>, <i>CAT</i>, <i>GSH-Px</i>, <i>HO-1</i>, <i>I-κBα,</i> and <i>Nrf2</i> (<i>p</i> < 0.05). The Cd-treated group showed significantly increased <i>NF-kBp65</i>, <i>TGF-β</i>, <i>IL-8</i>, <i>IL-1</i>, <i>Keap1,</i> and <i>TNF-α</i> expression levels. SL significantly alleviated the changes in the above indicators. The results of this study suggest that SL can trigger the Nrf2 signalling pathway and NF-kB signalling pathway to alleviate Cd toxicity. SL might be a potential drug for the treatment of Cd poisoning.https://www.mdpi.com/2410-3888/8/3/136Se-enriched <i>Lactobacillus plantarum</i>Cadmium<i>Luciobarbus capito</i>Oxidative stressInflammatory reactionBioaccumulation |
| spellingShingle | Qing Zhang Xinchi Shang Longwu Geng Xinghua Che Haijun Wei Shizhan Tang Wei Xu Dietary Selenium-Rich <i>Lactobacillus plantarum</i> Alleviates Cadmium-Induced Oxidative Stress and Inflammation in <i>Bulatmai barbel Luciobarbus capito</i> Fishes Se-enriched <i>Lactobacillus plantarum</i> Cadmium <i>Luciobarbus capito</i> Oxidative stress Inflammatory reaction Bioaccumulation |
| title | Dietary Selenium-Rich <i>Lactobacillus plantarum</i> Alleviates Cadmium-Induced Oxidative Stress and Inflammation in <i>Bulatmai barbel Luciobarbus capito</i> |
| title_full | Dietary Selenium-Rich <i>Lactobacillus plantarum</i> Alleviates Cadmium-Induced Oxidative Stress and Inflammation in <i>Bulatmai barbel Luciobarbus capito</i> |
| title_fullStr | Dietary Selenium-Rich <i>Lactobacillus plantarum</i> Alleviates Cadmium-Induced Oxidative Stress and Inflammation in <i>Bulatmai barbel Luciobarbus capito</i> |
| title_full_unstemmed | Dietary Selenium-Rich <i>Lactobacillus plantarum</i> Alleviates Cadmium-Induced Oxidative Stress and Inflammation in <i>Bulatmai barbel Luciobarbus capito</i> |
| title_short | Dietary Selenium-Rich <i>Lactobacillus plantarum</i> Alleviates Cadmium-Induced Oxidative Stress and Inflammation in <i>Bulatmai barbel Luciobarbus capito</i> |
| title_sort | dietary selenium rich i lactobacillus plantarum i alleviates cadmium induced oxidative stress and inflammation in i bulatmai barbel luciobarbus capito i |
| topic | Se-enriched <i>Lactobacillus plantarum</i> Cadmium <i>Luciobarbus capito</i> Oxidative stress Inflammatory reaction Bioaccumulation |
| url | https://www.mdpi.com/2410-3888/8/3/136 |
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