Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies
The Mediterranean fruit fly (medfly), <i>Ceratitis capitata</i>, is an agricultural pest of a wide range of fruits. The advent of high-throughput sequencing has boosted the discovery of RNA viruses infecting insects. In this article, we aim to characterize the RNA virome and viral sRNA p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/3/623 |
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| author | Luis Hernández-Pelegrín Ángel Llopis-Giménez Cristina Maria Crava Félix Ortego Pedro Hernández-Crespo Vera I. D. Ros Salvador Herrero |
| author_facet | Luis Hernández-Pelegrín Ángel Llopis-Giménez Cristina Maria Crava Félix Ortego Pedro Hernández-Crespo Vera I. D. Ros Salvador Herrero |
| author_sort | Luis Hernández-Pelegrín |
| collection | DOAJ |
| description | The Mediterranean fruit fly (medfly), <i>Ceratitis capitata</i>, is an agricultural pest of a wide range of fruits. The advent of high-throughput sequencing has boosted the discovery of RNA viruses infecting insects. In this article, we aim to characterize the RNA virome and viral sRNA profile of medfly. By means of transcriptome mining, we expanded the medfly RNA virome to 13 viruses, including two novel positive ssRNA viruses and the first two novel dsRNA viruses reported for medfly. Our analysis across multiple laboratory-reared and field-collected medfly samples showed the presence of a core RNA virome comprised of Ceratitis capitata iflavirus 2 and Ceratitis capitata negev-like virus 1. Furthermore, field-collected flies showed a higher viral diversity in comparison to the laboratory-reared flies. Based on the small RNA sequencing, we detected small interfering RNAs mapping to all the viruses present in each sample, except for Ceratitis capitata nora virus. Although the identified RNA viruses do not cause obvious symptoms in medflies, the outcome of their interaction may still influence the medfly’s fitness and ecology, becoming either a risk or an opportunity for mass-rearing and SIT applications. |
| first_indexed | 2024-03-09T12:15:39Z |
| format | Article |
| id | doaj.art-3888c5e40735414c91d346e9a2684e71 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T12:15:39Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-3888c5e40735414c91d346e9a2684e712023-11-30T22:47:14ZengMDPI AGViruses1999-49152022-03-0114362310.3390/v14030623Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived MedfliesLuis Hernández-Pelegrín0Ángel Llopis-Giménez1Cristina Maria Crava2Félix Ortego3Pedro Hernández-Crespo4Vera I. D. Ros5Salvador Herrero6Department of Genetics and University Institute of Biotechnology and Biomedicine (BIOTECMED), Universitat de València, 46100 Valencia, SpainDepartment of Genetics and University Institute of Biotechnology and Biomedicine (BIOTECMED), Universitat de València, 46100 Valencia, SpainDepartment of Genetics and University Institute of Biotechnology and Biomedicine (BIOTECMED), Universitat de València, 46100 Valencia, SpainDepartment of Microbial and Plant Biotechnology, Centro de Investigaciones Biológicas Margarita Salas, CSIC, 28040 Madrid, SpainDepartment of Microbial and Plant Biotechnology, Centro de Investigaciones Biológicas Margarita Salas, CSIC, 28040 Madrid, SpainLaboratory of Virology, Wageningen University and Research, Droevendaalsesteeg 1, 6708 PB Wageningen, The NetherlandsDepartment of Genetics and University Institute of Biotechnology and Biomedicine (BIOTECMED), Universitat de València, 46100 Valencia, SpainThe Mediterranean fruit fly (medfly), <i>Ceratitis capitata</i>, is an agricultural pest of a wide range of fruits. The advent of high-throughput sequencing has boosted the discovery of RNA viruses infecting insects. In this article, we aim to characterize the RNA virome and viral sRNA profile of medfly. By means of transcriptome mining, we expanded the medfly RNA virome to 13 viruses, including two novel positive ssRNA viruses and the first two novel dsRNA viruses reported for medfly. Our analysis across multiple laboratory-reared and field-collected medfly samples showed the presence of a core RNA virome comprised of Ceratitis capitata iflavirus 2 and Ceratitis capitata negev-like virus 1. Furthermore, field-collected flies showed a higher viral diversity in comparison to the laboratory-reared flies. Based on the small RNA sequencing, we detected small interfering RNAs mapping to all the viruses present in each sample, except for Ceratitis capitata nora virus. Although the identified RNA viruses do not cause obvious symptoms in medflies, the outcome of their interaction may still influence the medfly’s fitness and ecology, becoming either a risk or an opportunity for mass-rearing and SIT applications.https://www.mdpi.com/1999-4915/14/3/623Tephritidaesmall RNAnarnavirusnodavirusreovirustotivirus |
| spellingShingle | Luis Hernández-Pelegrín Ángel Llopis-Giménez Cristina Maria Crava Félix Ortego Pedro Hernández-Crespo Vera I. D. Ros Salvador Herrero Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies Viruses Tephritidae small RNA narnavirus nodavirus reovirus totivirus |
| title | Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies |
| title_full | Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies |
| title_fullStr | Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies |
| title_full_unstemmed | Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies |
| title_short | Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies |
| title_sort | expanding the medfly virome viral diversity prevalence and srna profiling in mass reared and field derived medflies |
| topic | Tephritidae small RNA narnavirus nodavirus reovirus totivirus |
| url | https://www.mdpi.com/1999-4915/14/3/623 |
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