HBME-1 immunostaining in reactive mesothelial versus metastatic adenocarcinoma cells in serous fluid
Background: The cytological diagnoses of serous effusions are usually made by routine cytomorphology with certainty. However, overlapping cases sometimes exist between reactive mesothelial and adenocarcinoma cells. We tried to evaluate the diagnostic utility of HBME-1 in distinguishing between react...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2011-01-01
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| Series: | Indian Journal of Pathology and Microbiology |
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| Online Access: | http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2011;volume=54;issue=3;spage=460;epage=463;aulast=Rahmani |
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| author | Alireza Rahmani Mohsen Zahedi Dehghani Noushin Moghaddam Afshar Hamidreza Heidarian Reza Tahririan |
| author_facet | Alireza Rahmani Mohsen Zahedi Dehghani Noushin Moghaddam Afshar Hamidreza Heidarian Reza Tahririan |
| author_sort | Alireza Rahmani |
| collection | DOAJ |
| description | Background: The cytological diagnoses of serous effusions are usually made by routine cytomorphology with certainty. However, overlapping cases sometimes exist between reactive mesothelial and adenocarcinoma cells. We tried to evaluate the diagnostic utility of HBME-1 in distinguishing between reactive mesothelial cells and adenocarcinoma in serous effusions. Materials and Methods: Fifty-two cytologic specimens processed by cell-block technique were retrieved from the archive and were assigned to two groups: Group I: 26 effusions containing reactive/benign mesothelial cells; Group II: 26 effusions containing carcinoma cells. Immunostaining with HBME-1 was performed using an Envision technique. The staining intensity of cells, according to proportion of immunoreactive cells, was scored as: 0 (negative), 1+ (<10%), 2+ (10-50%), and 3+ (≥50%); and the predominant staining pattern of positive cells were determined. Statistical analysis and tests of significance were performed using SPSS software. Results: The calculated mean values (in percentile) for stained cells in adenocarcinoma and reactive mesothelial cells were 25.57 and 67.88, respectively ( P = 0.001). Thin membranous, thick membranous, thick and thin membranous, cytoplasmic and combined patterns of staining in adenocarcinoma cells were respectively 4 cases (21.1%), 0 case, 0 case, 8 cases (42.1%), and 7 cases (36.8%), and in reactive mesothelial cells, these were 7 cases (26.9%), 1 case (3.8%), 18 cases (69.2%), 0 case, and 0 case, respectively. The intensity of staining in majority (88.5%) of reactive mesothelial cells was scored 3+, but the distribution varied in the other group. Conclusions: The staining pattern and intensity for HBME-1 is a useful panel for differentiation of adenocarcinoma and mesothelial cells. |
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| id | doaj.art-388e21ddd4d14d93ad3f821c138d97e3 |
| institution | Directory Open Access Journal |
| issn | 0377-4929 |
| language | English |
| last_indexed | 2024-12-12T01:38:46Z |
| publishDate | 2011-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Pathology and Microbiology |
| spelling | doaj.art-388e21ddd4d14d93ad3f821c138d97e32022-12-22T00:42:46ZengWolters Kluwer Medknow PublicationsIndian Journal of Pathology and Microbiology0377-49292011-01-0154346046310.4103/0377-4929.85075HBME-1 immunostaining in reactive mesothelial versus metastatic adenocarcinoma cells in serous fluidAlireza RahmaniMohsen Zahedi DehghaniNoushin Moghaddam AfsharHamidreza HeidarianReza TahririanBackground: The cytological diagnoses of serous effusions are usually made by routine cytomorphology with certainty. However, overlapping cases sometimes exist between reactive mesothelial and adenocarcinoma cells. We tried to evaluate the diagnostic utility of HBME-1 in distinguishing between reactive mesothelial cells and adenocarcinoma in serous effusions. Materials and Methods: Fifty-two cytologic specimens processed by cell-block technique were retrieved from the archive and were assigned to two groups: Group I: 26 effusions containing reactive/benign mesothelial cells; Group II: 26 effusions containing carcinoma cells. Immunostaining with HBME-1 was performed using an Envision technique. The staining intensity of cells, according to proportion of immunoreactive cells, was scored as: 0 (negative), 1+ (<10%), 2+ (10-50%), and 3+ (≥50%); and the predominant staining pattern of positive cells were determined. Statistical analysis and tests of significance were performed using SPSS software. Results: The calculated mean values (in percentile) for stained cells in adenocarcinoma and reactive mesothelial cells were 25.57 and 67.88, respectively ( P = 0.001). Thin membranous, thick membranous, thick and thin membranous, cytoplasmic and combined patterns of staining in adenocarcinoma cells were respectively 4 cases (21.1%), 0 case, 0 case, 8 cases (42.1%), and 7 cases (36.8%), and in reactive mesothelial cells, these were 7 cases (26.9%), 1 case (3.8%), 18 cases (69.2%), 0 case, and 0 case, respectively. The intensity of staining in majority (88.5%) of reactive mesothelial cells was scored 3+, but the distribution varied in the other group. Conclusions: The staining pattern and intensity for HBME-1 is a useful panel for differentiation of adenocarcinoma and mesothelial cells.http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2011;volume=54;issue=3;spage=460;epage=463;aulast=RahmaniCytologyHBME-1 antibodymetastatic adenocarcinomareactive mesothelial cellserous effusion |
| spellingShingle | Alireza Rahmani Mohsen Zahedi Dehghani Noushin Moghaddam Afshar Hamidreza Heidarian Reza Tahririan HBME-1 immunostaining in reactive mesothelial versus metastatic adenocarcinoma cells in serous fluid Indian Journal of Pathology and Microbiology Cytology HBME-1 antibody metastatic adenocarcinoma reactive mesothelial cell serous effusion |
| title | HBME-1 immunostaining in reactive mesothelial versus metastatic adenocarcinoma cells in serous fluid |
| title_full | HBME-1 immunostaining in reactive mesothelial versus metastatic adenocarcinoma cells in serous fluid |
| title_fullStr | HBME-1 immunostaining in reactive mesothelial versus metastatic adenocarcinoma cells in serous fluid |
| title_full_unstemmed | HBME-1 immunostaining in reactive mesothelial versus metastatic adenocarcinoma cells in serous fluid |
| title_short | HBME-1 immunostaining in reactive mesothelial versus metastatic adenocarcinoma cells in serous fluid |
| title_sort | hbme 1 immunostaining in reactive mesothelial versus metastatic adenocarcinoma cells in serous fluid |
| topic | Cytology HBME-1 antibody metastatic adenocarcinoma reactive mesothelial cell serous effusion |
| url | http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2011;volume=54;issue=3;spage=460;epage=463;aulast=Rahmani |
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