Production of Ligninolytic Enzymes by Coptotermes curvignathus Gut Bacteria
Maximum utilization of lignocellulosic biomass is contingent upon degrading the recalcitrant lignin polymer. Conventional methods employed in delignification require high inputs of energy and chemicals, resulting in the release of highly toxic effluents. The ability of gut flora of Coptotermes curvi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2019-01-01
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| Series: | Environmental and Climate Technologies |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/rtuect-2019-0008 |
| _version_ | 1819090232410112000 |
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| author | Ayeronfe Fadilah Kassim Angzzas Hung Patricia Ishak Nadiah Syarifah Sharfina Aripin Ashuvila |
| author_facet | Ayeronfe Fadilah Kassim Angzzas Hung Patricia Ishak Nadiah Syarifah Sharfina Aripin Ashuvila |
| author_sort | Ayeronfe Fadilah |
| collection | DOAJ |
| description | Maximum utilization of lignocellulosic biomass is contingent upon degrading the recalcitrant lignin polymer. Conventional methods employed in delignification require high inputs of energy and chemicals, resulting in the release of highly toxic effluents. The ability of gut flora of Coptotermes curvignathus in lignin degradation was investigated in this study. Production of ligninolytic enzymes was done in an aerated submerged fermentation system with kraft lignin as sole carbon source. The degradation experiment was carried out for 7 days at 30 °C, pH 7. Three potential lignin degraders identified as Bacillus sp., Lysinibacillus sp. and Acinetobacter sp. were successfully isolated. The bacterial growth and secretion of extracellular ligninolytic enzymes confirmed metabolism of kraft lignin by the identified strains. Lysinibacillus sp., a novel lignin degrader showed highest manganese peroxidase (76.36 ± 15.74 U/L) and laccase activity (70.67 ± 16.82 U/L) after 7 and 6 days of incubation respectively, while maximal activity of lignin peroxidase (262.49 ± 0.92 U/L) was recorded after 7 days in culture supernatants of Bacillus sp. With respect to the activity of the secreted enzymes, the lignin degrading potential of these bacterial strains can be explored in the valorisations of lignocellulosic biomass in industrial processes such as pulping, bioethanol production, fine chemicals and materials synthesis. |
| first_indexed | 2024-12-21T22:20:33Z |
| format | Article |
| id | doaj.art-38907a3ca71c4c83b1c182cdc2e2ad30 |
| institution | Directory Open Access Journal |
| issn | 2255-8837 |
| language | English |
| last_indexed | 2024-12-21T22:20:33Z |
| publishDate | 2019-01-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Environmental and Climate Technologies |
| spelling | doaj.art-38907a3ca71c4c83b1c182cdc2e2ad302022-12-21T18:48:20ZengSciendoEnvironmental and Climate Technologies2255-88372019-01-0123111112110.2478/rtuect-2019-0008rtuect-2019-0008Production of Ligninolytic Enzymes by Coptotermes curvignathus Gut BacteriaAyeronfe Fadilah0Kassim Angzzas1Hung Patricia2Ishak Nadiah3Syarifah Sharfina4Aripin Ashuvila5Department of Chemical Engineering Technology, Faculty of Engineering Technology, University Tun Hussein Onn Malaysia, Batu Pahat, Johor, 86400, MalaysiaDepartment of Chemical Engineering Technology, Faculty of Engineering Technology, University Tun Hussein Onn Malaysia, Batu Pahat, Johor, 86400, MalaysiaDepartment of Crop Science, Faculty of Agriculture and Food Sciences, University Putra Malaysia, Bintulu, Sarawak, 97008, MalaysiaDepartment of Chemical Engineering Technology, Faculty of Engineering Technology, University Tun Hussein Onn Malaysia, Batu Pahat, Johor, 86400, MalaysiaDepartment of Chemical Engineering Technology, Faculty of Engineering Technology, University Tun Hussein Onn Malaysia, Batu Pahat, Johor, 86400, MalaysiaDepartment of Chemical Engineering Technology, Faculty of Engineering Technology, University Tun Hussein Onn Malaysia, Batu Pahat, Johor, 86400, MalaysiaMaximum utilization of lignocellulosic biomass is contingent upon degrading the recalcitrant lignin polymer. Conventional methods employed in delignification require high inputs of energy and chemicals, resulting in the release of highly toxic effluents. The ability of gut flora of Coptotermes curvignathus in lignin degradation was investigated in this study. Production of ligninolytic enzymes was done in an aerated submerged fermentation system with kraft lignin as sole carbon source. The degradation experiment was carried out for 7 days at 30 °C, pH 7. Three potential lignin degraders identified as Bacillus sp., Lysinibacillus sp. and Acinetobacter sp. were successfully isolated. The bacterial growth and secretion of extracellular ligninolytic enzymes confirmed metabolism of kraft lignin by the identified strains. Lysinibacillus sp., a novel lignin degrader showed highest manganese peroxidase (76.36 ± 15.74 U/L) and laccase activity (70.67 ± 16.82 U/L) after 7 and 6 days of incubation respectively, while maximal activity of lignin peroxidase (262.49 ± 0.92 U/L) was recorded after 7 days in culture supernatants of Bacillus sp. With respect to the activity of the secreted enzymes, the lignin degrading potential of these bacterial strains can be explored in the valorisations of lignocellulosic biomass in industrial processes such as pulping, bioethanol production, fine chemicals and materials synthesis.https://doi.org/10.2478/rtuect-2019-0008enzymeskraft ligninlaccaseperoxidasestermites |
| spellingShingle | Ayeronfe Fadilah Kassim Angzzas Hung Patricia Ishak Nadiah Syarifah Sharfina Aripin Ashuvila Production of Ligninolytic Enzymes by Coptotermes curvignathus Gut Bacteria Environmental and Climate Technologies enzymes kraft lignin laccase peroxidases termites |
| title | Production of Ligninolytic Enzymes by Coptotermes curvignathus Gut Bacteria |
| title_full | Production of Ligninolytic Enzymes by Coptotermes curvignathus Gut Bacteria |
| title_fullStr | Production of Ligninolytic Enzymes by Coptotermes curvignathus Gut Bacteria |
| title_full_unstemmed | Production of Ligninolytic Enzymes by Coptotermes curvignathus Gut Bacteria |
| title_short | Production of Ligninolytic Enzymes by Coptotermes curvignathus Gut Bacteria |
| title_sort | production of ligninolytic enzymes by coptotermes curvignathus gut bacteria |
| topic | enzymes kraft lignin laccase peroxidases termites |
| url | https://doi.org/10.2478/rtuect-2019-0008 |
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