Serotypes and Stx2 subtyping of Shiga toxin producing Escherichia coli isolates from cattle carcasses and feces
Shiga toxin E. coli (STEC) is an important pathogen responsible for foodborne illness, this have been related with epidemic outbreaks in the past, mainly because of consumption of bovine meat. The objective of this study was identify the serotypes and Stx2 subtypes and associate them with their poss...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto Nacional del Investigaciones Forestales, Agrícolas y Pecuarias
2020-10-01
|
| Series: | Revista Mexicana de Ciencias Pecuarias |
| Subjects: | |
| Online Access: | https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5049/5105 |
| _version_ | 1797903717135024128 |
|---|---|
| author | Nydia Edith Reyes-Rodriguez Jeannette Barba-León Armando Navarro-Ocaña Vicente Vega-Sanchez Fabian Ricardo Gómez De Anda Juan Martin Talavera-Gonzalez Martin Talavera-Rojas |
| author_facet | Nydia Edith Reyes-Rodriguez Jeannette Barba-León Armando Navarro-Ocaña Vicente Vega-Sanchez Fabian Ricardo Gómez De Anda Juan Martin Talavera-Gonzalez Martin Talavera-Rojas |
| author_sort | Nydia Edith Reyes-Rodriguez |
| collection | DOAJ |
| description | Shiga toxin E. coli (STEC) is an important pathogen responsible for foodborne illness, this have been related with epidemic outbreaks in the past, mainly because of consumption of bovine meat. The objective of this study was identify the serotypes and Stx2 subtypes and associate them with their possible epidemiology. There were analyzed a total of 65 isolates from the collection of the Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia of the Universidad Autónoma del Estado de México, from carcasses and feces of bovines at three different Municipal slaughterhouses. The identification of Stx2 gene by PCR at final point, sequencing and analyzed with the help of BLAST software. There were found O157:H7, O70:H16, O91:H10, O112ac:H2, O128ac:H26 serotypes, which have been reported to be present at infectious outbreaks previously by foodborne worldwide; 63.07% (41/65) of the Escherichia coli strains got amplified for Stx2 and after BLAS analysis it was confirmed its presence and a hypothetic protein. The presence of this serotypes in combination with different subtype’s, Stx2a, Stx2c, Stx2d, in carcasses and feces of bovine in must be considered as a potential risk for diseases an important problem of the public health. |
| first_indexed | 2024-04-10T09:37:22Z |
| format | Article |
| id | doaj.art-38933c620aed4035a29cc9a29319836a |
| institution | Directory Open Access Journal |
| issn | 2007-1124 2448-6698 |
| language | English |
| last_indexed | 2024-04-10T09:37:22Z |
| publishDate | 2020-10-01 |
| publisher | Instituto Nacional del Investigaciones Forestales, Agrícolas y Pecuarias |
| record_format | Article |
| series | Revista Mexicana de Ciencias Pecuarias |
| spelling | doaj.art-38933c620aed4035a29cc9a29319836a2023-02-17T17:28:52ZengInstituto Nacional del Investigaciones Forestales, Agrícolas y PecuariasRevista Mexicana de Ciencias Pecuarias2007-11242448-66982020-10-0111410301044https://doi.org/10.22319/rmcp.v11i4.5049Serotypes and Stx2 subtyping of Shiga toxin producing Escherichia coli isolates from cattle carcasses and fecesNydia Edith Reyes-Rodriguez0Jeannette Barba-León1Armando Navarro-Ocaña2Vicente Vega-Sanchez3Fabian Ricardo Gómez De Anda4Juan Martin Talavera-Gonzalez5Martin Talavera-Rojas6https://orcid.org/0000-0003-0908-985XUniversidad Autónoma del Estado de Hidalgo. Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia. Tulancingo de Bravo, Estado de Hidalgo, México.Universidad de Guadalajara. Centro Universitario de Ciencias Biológicas y Agropecuarias, Departamento de Salud Pública. Zapopan, Jalisco, México.Universidad Nacional Autónoma de México. Facultad de Medicina, Departamento de Salud Pública. Ciudad Universitaria, México.Universidad Autónoma del Estado de Hidalgo. Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia. Tulancingo de Bravo, Estado de Hidalgo, México.Universidad Autónoma del Estado de Hidalgo. Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia. Tulancingo de Bravo, Estado de Hidalgo, México. Universidad Autónoma del Estado de México. Facultad de Medicina Veterinaria y Zootecnia, Centro de Investigación y Estudios Avanzados en Salud Animal. Toluca, Estado de México, México. Universidad Autónoma del Estado de México. Facultad de Medicina Veterinaria y Zootecnia, Centro de Investigación y Estudios Avanzados en Salud Animal. Toluca, Estado de México, México.Shiga toxin E. coli (STEC) is an important pathogen responsible for foodborne illness, this have been related with epidemic outbreaks in the past, mainly because of consumption of bovine meat. The objective of this study was identify the serotypes and Stx2 subtypes and associate them with their possible epidemiology. There were analyzed a total of 65 isolates from the collection of the Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia of the Universidad Autónoma del Estado de México, from carcasses and feces of bovines at three different Municipal slaughterhouses. The identification of Stx2 gene by PCR at final point, sequencing and analyzed with the help of BLAST software. There were found O157:H7, O70:H16, O91:H10, O112ac:H2, O128ac:H26 serotypes, which have been reported to be present at infectious outbreaks previously by foodborne worldwide; 63.07% (41/65) of the Escherichia coli strains got amplified for Stx2 and after BLAS analysis it was confirmed its presence and a hypothetic protein. The presence of this serotypes in combination with different subtype’s, Stx2a, Stx2c, Stx2d, in carcasses and feces of bovine in must be considered as a potential risk for diseases an important problem of the public health.https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5049/5105cattle carcassesescherichia coliserotypingstx2 |
| spellingShingle | Nydia Edith Reyes-Rodriguez Jeannette Barba-León Armando Navarro-Ocaña Vicente Vega-Sanchez Fabian Ricardo Gómez De Anda Juan Martin Talavera-Gonzalez Martin Talavera-Rojas Serotypes and Stx2 subtyping of Shiga toxin producing Escherichia coli isolates from cattle carcasses and feces Revista Mexicana de Ciencias Pecuarias cattle carcasses escherichia coli serotyping stx2 |
| title | Serotypes and Stx2 subtyping of Shiga toxin producing Escherichia coli isolates from cattle carcasses and feces |
| title_full | Serotypes and Stx2 subtyping of Shiga toxin producing Escherichia coli isolates from cattle carcasses and feces |
| title_fullStr | Serotypes and Stx2 subtyping of Shiga toxin producing Escherichia coli isolates from cattle carcasses and feces |
| title_full_unstemmed | Serotypes and Stx2 subtyping of Shiga toxin producing Escherichia coli isolates from cattle carcasses and feces |
| title_short | Serotypes and Stx2 subtyping of Shiga toxin producing Escherichia coli isolates from cattle carcasses and feces |
| title_sort | serotypes and stx2 subtyping of shiga toxin producing escherichia coli isolates from cattle carcasses and feces |
| topic | cattle carcasses escherichia coli serotyping stx2 |
| url | https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5049/5105 |
| work_keys_str_mv | AT nydiaedithreyesrodriguez serotypesandstx2subtypingofshigatoxinproducingescherichiacoliisolatesfromcattlecarcassesandfeces AT jeannettebarbaleon serotypesandstx2subtypingofshigatoxinproducingescherichiacoliisolatesfromcattlecarcassesandfeces AT armandonavarroocana serotypesandstx2subtypingofshigatoxinproducingescherichiacoliisolatesfromcattlecarcassesandfeces AT vicentevegasanchez serotypesandstx2subtypingofshigatoxinproducingescherichiacoliisolatesfromcattlecarcassesandfeces AT fabianricardogomezdeanda serotypesandstx2subtypingofshigatoxinproducingescherichiacoliisolatesfromcattlecarcassesandfeces AT juanmartintalaveragonzalez serotypesandstx2subtypingofshigatoxinproducingescherichiacoliisolatesfromcattlecarcassesandfeces AT martintalaverarojas serotypesandstx2subtypingofshigatoxinproducingescherichiacoliisolatesfromcattlecarcassesandfeces |