Efek Imunomodulator Ekstrak Etanol Spons Melophlus sarasinorum Terhadap Aktivitas Fagositosis Sel Makrofag Pada Mencit Jantan Balb/C
Immunomodulator is an ingredient or drug that can modulate immune system functions and activities. This study was conducted to determine the effect of ethanol extract of Melophlus sarasinorum sponge on macrophage phagocytosis activity. Twenty four male mice balb/c were divided into six groups. The f...
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| Format: | Article |
| Language: | English |
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Universitas Tadulako
2019-10-01
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| Series: | Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) |
| Subjects: | |
| Online Access: | http://jurnal.untad.ac.id/jurnal/index.php/Galenika/article/view/13611 |
| _version_ | 1811295881450749952 |
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| author | Wahyuni Wahyuni Muhammad Ilyas Yusuf Fadhliyah Malik Adryan Fristiohady Lubis Astrid Indalifiany I Sahidin |
| author_facet | Wahyuni Wahyuni Muhammad Ilyas Yusuf Fadhliyah Malik Adryan Fristiohady Lubis Astrid Indalifiany I Sahidin |
| author_sort | Wahyuni Wahyuni |
| collection | DOAJ |
| description | Immunomodulator is an ingredient or drug that can modulate immune system functions and activities. This study was conducted to determine the effect of ethanol extract of Melophlus sarasinorum sponge on macrophage phagocytosis activity. Twenty four male mice balb/c were divided into six groups. The first group received 100 mg/kg of ethanol extract of Melophlus Sarasinorum sponge, the second group received 200 mg/kg of ethanol extract of Melophlus sarasinorum sponge, the third group received 300 mg/kg of ethanol extract of Melophlus Sarasinorum sponge and the fourth group received 400 mg/kg of ethanol extract of Melophlus Sarasinorum sponge. The positive control group received Phyllanthus niruri linn extract (Stimuno®) 0,13 mg/g and the negative control group received NaCMC 0,5%. The extract was orally administered from first day to seventh day. On the eighth day, each of the mice was injected Staphylococcus aureus bacteria (SA) 0.5 mL intraperitoneally. Macrophage cell activity is calculated from smears of peritoneal fluid of mice. Increased doses of ethanol extract of Melophlus sarasinorum sponge increase the amount of macrophage phagocytosis activity that are 25,25% (negative control), 61,5% (positive control), 55,75% (100 mg/kg), 60,75% (200 mg/kg), 62,25% (300 mg/kg) dan 66,25% (400 mg/kg). The results showed that the ethanol extract of Melophlus sarasinorum sponge has the potential as immunomodulator at a doses of 300 mg/kgBB and 400 mg/kg with no significantly different effectiveness with positive control in increasing macrophage cell phagocytosis activity based on the result of post-hoc statistical test of Tukey (sig.> 0,05). |
| first_indexed | 2024-04-13T05:40:09Z |
| format | Article |
| id | doaj.art-38949c08392648dea620b58bba08fd80 |
| institution | Directory Open Access Journal |
| issn | 2442-7284 2442-8744 |
| language | English |
| last_indexed | 2024-04-13T05:40:09Z |
| publishDate | 2019-10-01 |
| publisher | Universitas Tadulako |
| record_format | Article |
| series | Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) |
| spelling | doaj.art-38949c08392648dea620b58bba08fd802022-12-22T03:00:08ZengUniversitas TadulakoJurnal Farmasi Galenika (Galenika Journal of Pharmacy)2442-72842442-87442019-10-015214715710.22487/j24428744.2019.v5.i2.1361110638Efek Imunomodulator Ekstrak Etanol Spons Melophlus sarasinorum Terhadap Aktivitas Fagositosis Sel Makrofag Pada Mencit Jantan Balb/CWahyuni Wahyuni0Muhammad Ilyas Yusuf1Fadhliyah Malik2Adryan Fristiohady Lubis3Astrid Indalifiany4I Sahidin5Jurusan Farmasi Fakultas Farmasi Universitas Halu Oleo, Kendari, Jl. H.E.A Mokodompit Kampus Hijau Bumi Tridharma, Kendari, Sulawesi TenggaraJurusan Farmasi Fakultas Farmasi Universitas Halu Oleo, Kendari, Jl. H.E.A Mokodompit Kampus Hijau Bumi Tridharma, Kendari, Sulawesi Tenggara/Politeknik Bina Husada Kendari, Jl. Sorumba No.17 Kendari-Sulawesi TenggaraJurusan Farmasi Fakultas Farmasi Universitas Halu Oleo, Kendari, Jl. H.E.A Mokodompit Kampus Hijau Bumi Tridharma, Kendari, Sulawesi TenggaraJurusan Farmasi Fakultas Farmasi Universitas Halu Oleo, Kendari, Jl. H.E.A Mokodompit Kampus Hijau Bumi Tridharma, Kendari, Sulawesi TenggaraJurusan Farmasi Fakultas Farmasi Universitas Halu Oleo, Kendari, Jl. H.E.A Mokodompit Kampus Hijau Bumi Tridharma, Kendari, Sulawesi TenggaraJurusan Farmasi Fakultas Farmasi Universitas Halu Oleo, Kendari, Jl. H.E.A Mokodompit Kampus Hijau Bumi Tridharma, Kendari, Sulawesi TenggaraImmunomodulator is an ingredient or drug that can modulate immune system functions and activities. This study was conducted to determine the effect of ethanol extract of Melophlus sarasinorum sponge on macrophage phagocytosis activity. Twenty four male mice balb/c were divided into six groups. The first group received 100 mg/kg of ethanol extract of Melophlus Sarasinorum sponge, the second group received 200 mg/kg of ethanol extract of Melophlus sarasinorum sponge, the third group received 300 mg/kg of ethanol extract of Melophlus Sarasinorum sponge and the fourth group received 400 mg/kg of ethanol extract of Melophlus Sarasinorum sponge. The positive control group received Phyllanthus niruri linn extract (Stimuno®) 0,13 mg/g and the negative control group received NaCMC 0,5%. The extract was orally administered from first day to seventh day. On the eighth day, each of the mice was injected Staphylococcus aureus bacteria (SA) 0.5 mL intraperitoneally. Macrophage cell activity is calculated from smears of peritoneal fluid of mice. Increased doses of ethanol extract of Melophlus sarasinorum sponge increase the amount of macrophage phagocytosis activity that are 25,25% (negative control), 61,5% (positive control), 55,75% (100 mg/kg), 60,75% (200 mg/kg), 62,25% (300 mg/kg) dan 66,25% (400 mg/kg). The results showed that the ethanol extract of Melophlus sarasinorum sponge has the potential as immunomodulator at a doses of 300 mg/kgBB and 400 mg/kg with no significantly different effectiveness with positive control in increasing macrophage cell phagocytosis activity based on the result of post-hoc statistical test of Tukey (sig.> 0,05).http://jurnal.untad.ac.id/jurnal/index.php/Galenika/article/view/13611sponge melophlus sarasinorum., extract, macrophage, immunomodulator |
| spellingShingle | Wahyuni Wahyuni Muhammad Ilyas Yusuf Fadhliyah Malik Adryan Fristiohady Lubis Astrid Indalifiany I Sahidin Efek Imunomodulator Ekstrak Etanol Spons Melophlus sarasinorum Terhadap Aktivitas Fagositosis Sel Makrofag Pada Mencit Jantan Balb/C Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) sponge melophlus sarasinorum., extract, macrophage, immunomodulator |
| title | Efek Imunomodulator Ekstrak Etanol Spons Melophlus sarasinorum Terhadap Aktivitas Fagositosis Sel Makrofag Pada Mencit Jantan Balb/C |
| title_full | Efek Imunomodulator Ekstrak Etanol Spons Melophlus sarasinorum Terhadap Aktivitas Fagositosis Sel Makrofag Pada Mencit Jantan Balb/C |
| title_fullStr | Efek Imunomodulator Ekstrak Etanol Spons Melophlus sarasinorum Terhadap Aktivitas Fagositosis Sel Makrofag Pada Mencit Jantan Balb/C |
| title_full_unstemmed | Efek Imunomodulator Ekstrak Etanol Spons Melophlus sarasinorum Terhadap Aktivitas Fagositosis Sel Makrofag Pada Mencit Jantan Balb/C |
| title_short | Efek Imunomodulator Ekstrak Etanol Spons Melophlus sarasinorum Terhadap Aktivitas Fagositosis Sel Makrofag Pada Mencit Jantan Balb/C |
| title_sort | efek imunomodulator ekstrak etanol spons melophlus sarasinorum terhadap aktivitas fagositosis sel makrofag pada mencit jantan balb c |
| topic | sponge melophlus sarasinorum., extract, macrophage, immunomodulator |
| url | http://jurnal.untad.ac.id/jurnal/index.php/Galenika/article/view/13611 |
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