Occurrence of antibiotic resistance genes and potentially pathogenic bacteria in the Yangtze River tributary (Nanjing section) and their correlation with environmental factors
Freshwater environments are vulnerable to emerging contaminants such as Antibiotic resistance genes (ARGs), and their occurrence is gaining more attention. However, the occurrence of ARGs along with potential pathogens is less explored. The current study aimed to evaluate the abundance of ARGs and e...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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IOP Publishing
2023-01-01
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| Series: | Environmental Research Communications |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/2515-7620/acbd8c |
| _version_ | 1797844650873061376 |
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| author | Adharsh Rajasekar Kiran Kumar Vadde Raphinos Tackmore Murava Mengru Qiu Shuaizhi Guo Tong Yu Ruoqing Wang Cailin Zhao |
| author_facet | Adharsh Rajasekar Kiran Kumar Vadde Raphinos Tackmore Murava Mengru Qiu Shuaizhi Guo Tong Yu Ruoqing Wang Cailin Zhao |
| author_sort | Adharsh Rajasekar |
| collection | DOAJ |
| description | Freshwater environments are vulnerable to emerging contaminants such as Antibiotic resistance genes (ARGs), and their occurrence is gaining more attention. However, the occurrence of ARGs along with potential pathogens is less explored. The current study aimed to evaluate the abundance of ARGs and explore bacterial communities for the presence of potential bacterial pathogens in water samples collected from a tributary to the Yangtze River in Nanjing. Twelve physico-chemical parameters were analyzed, followed by quantifying 10 ARGs targeting sulfonamide ( sul1, sul2 ), tetracycline ( tetG, tetM, tetQ ), erythromycin ( ermB ), vancomycin ( vanA, vanR ), and streptomycin ( strA, strB ) using real-time PCR and bacterial diversity characterization using high-throughput 16 S rRNA sequencing. The results indicated poor water quality and high-level eutrophication in most sampling locations. sul1 , sul2 , and strB were dominant in the study area with average concentrations of 6.8, 7.1, and 6.5 Log _10 gene copies/100 ml, respectively. Proteobacteria, Cyanobacteria, Bacteroidetes, and Actinobacteria were the main phyla detected in the study area, and genus-level analysis revealed the presence of eight potential pathogenic and ten fecal-associated bacterial genera at several locations in the study area. The distance-based Redundancy analysis indicated that total phosphorus, electrical conductivity, dissolved oxygen, total dissolved solids, ammonium-N (NH _4 ^+ -N), and chlorophyll a had significantly influenced the bacterial community composition in the monitored locations. Correlation analysis demonstrated that water temperature, pH, NH _4 ^+ -N, and total organic carbon were positively correlated with sul2 , tetG , and vanR genes, indicating that these environmental parameters significantly affected the ARGs distribution pattern. Overall, our results provide valuable information regarding the occurrence of ARGs and potential bacterial pathogens in the study area; however, their co-existence highlights increased human health risks. |
| first_indexed | 2024-04-09T17:25:43Z |
| format | Article |
| id | doaj.art-3895509959334ba19af5f23270f7a1a6 |
| institution | Directory Open Access Journal |
| issn | 2515-7620 |
| language | English |
| last_indexed | 2024-04-09T17:25:43Z |
| publishDate | 2023-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | Environmental Research Communications |
| spelling | doaj.art-3895509959334ba19af5f23270f7a1a62023-04-18T13:48:32ZengIOP PublishingEnvironmental Research Communications2515-76202023-01-015303500110.1088/2515-7620/acbd8cOccurrence of antibiotic resistance genes and potentially pathogenic bacteria in the Yangtze River tributary (Nanjing section) and their correlation with environmental factorsAdharsh Rajasekar0https://orcid.org/0000-0002-1917-0494Kiran Kumar Vadde1Raphinos Tackmore Murava2Mengru Qiu3Shuaizhi Guo4Tong Yu5Ruoqing Wang6Cailin Zhao7https://orcid.org/0000-0002-9236-8333Schoool of Geography and Environmental Sciences, University of Reading , Reading RG6 6AH , United Kingdom; Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control (AEMPC), Collaborative Innovation Center of Atmospheric Environment and Equipment Technology (CIC-AEET), Nanjing University of Information Science &Technology , Nanjing 210044, People’s Republic of ChinaSchool of Civil & Environmental Engineering, and Construction Management, University of Texas at San Antonio , San Antonio, TX 78249, United States of AmericaJiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control (AEMPC), Collaborative Innovation Center of Atmospheric Environment and Equipment Technology (CIC-AEET), Nanjing University of Information Science &Technology , Nanjing 210044, People’s Republic of ChinaJiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control (AEMPC), Collaborative Innovation Center of Atmospheric Environment and Equipment Technology (CIC-AEET), Nanjing University of Information Science &Technology , Nanjing 210044, People’s Republic of ChinaJiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control (AEMPC), Collaborative Innovation Center of Atmospheric Environment and Equipment Technology (CIC-AEET), Nanjing University of Information Science &Technology , Nanjing 210044, People’s Republic of ChinaJiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control (AEMPC), Collaborative Innovation Center of Atmospheric Environment and Equipment Technology (CIC-AEET), Nanjing University of Information Science &Technology , Nanjing 210044, People’s Republic of ChinaJiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control (AEMPC), Collaborative Innovation Center of Atmospheric Environment and Equipment Technology (CIC-AEET), Nanjing University of Information Science &Technology , Nanjing 210044, People’s Republic of ChinaJiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control (AEMPC), Collaborative Innovation Center of Atmospheric Environment and Equipment Technology (CIC-AEET), Nanjing University of Information Science &Technology , Nanjing 210044, People’s Republic of ChinaFreshwater environments are vulnerable to emerging contaminants such as Antibiotic resistance genes (ARGs), and their occurrence is gaining more attention. However, the occurrence of ARGs along with potential pathogens is less explored. The current study aimed to evaluate the abundance of ARGs and explore bacterial communities for the presence of potential bacterial pathogens in water samples collected from a tributary to the Yangtze River in Nanjing. Twelve physico-chemical parameters were analyzed, followed by quantifying 10 ARGs targeting sulfonamide ( sul1, sul2 ), tetracycline ( tetG, tetM, tetQ ), erythromycin ( ermB ), vancomycin ( vanA, vanR ), and streptomycin ( strA, strB ) using real-time PCR and bacterial diversity characterization using high-throughput 16 S rRNA sequencing. The results indicated poor water quality and high-level eutrophication in most sampling locations. sul1 , sul2 , and strB were dominant in the study area with average concentrations of 6.8, 7.1, and 6.5 Log _10 gene copies/100 ml, respectively. Proteobacteria, Cyanobacteria, Bacteroidetes, and Actinobacteria were the main phyla detected in the study area, and genus-level analysis revealed the presence of eight potential pathogenic and ten fecal-associated bacterial genera at several locations in the study area. The distance-based Redundancy analysis indicated that total phosphorus, electrical conductivity, dissolved oxygen, total dissolved solids, ammonium-N (NH _4 ^+ -N), and chlorophyll a had significantly influenced the bacterial community composition in the monitored locations. Correlation analysis demonstrated that water temperature, pH, NH _4 ^+ -N, and total organic carbon were positively correlated with sul2 , tetG , and vanR genes, indicating that these environmental parameters significantly affected the ARGs distribution pattern. Overall, our results provide valuable information regarding the occurrence of ARGs and potential bacterial pathogens in the study area; however, their co-existence highlights increased human health risks.https://doi.org/10.1088/2515-7620/acbd8cyangtze riverantibiotic-resistant genesbacterial diversitybacterial pathogens |
| spellingShingle | Adharsh Rajasekar Kiran Kumar Vadde Raphinos Tackmore Murava Mengru Qiu Shuaizhi Guo Tong Yu Ruoqing Wang Cailin Zhao Occurrence of antibiotic resistance genes and potentially pathogenic bacteria in the Yangtze River tributary (Nanjing section) and their correlation with environmental factors Environmental Research Communications yangtze river antibiotic-resistant genes bacterial diversity bacterial pathogens |
| title | Occurrence of antibiotic resistance genes and potentially pathogenic bacteria in the Yangtze River tributary (Nanjing section) and their correlation with environmental factors |
| title_full | Occurrence of antibiotic resistance genes and potentially pathogenic bacteria in the Yangtze River tributary (Nanjing section) and their correlation with environmental factors |
| title_fullStr | Occurrence of antibiotic resistance genes and potentially pathogenic bacteria in the Yangtze River tributary (Nanjing section) and their correlation with environmental factors |
| title_full_unstemmed | Occurrence of antibiotic resistance genes and potentially pathogenic bacteria in the Yangtze River tributary (Nanjing section) and their correlation with environmental factors |
| title_short | Occurrence of antibiotic resistance genes and potentially pathogenic bacteria in the Yangtze River tributary (Nanjing section) and their correlation with environmental factors |
| title_sort | occurrence of antibiotic resistance genes and potentially pathogenic bacteria in the yangtze river tributary nanjing section and their correlation with environmental factors |
| topic | yangtze river antibiotic-resistant genes bacterial diversity bacterial pathogens |
| url | https://doi.org/10.1088/2515-7620/acbd8c |
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