Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection
Abstract Background Live anticoccidial vaccines have been a tremendous success for disease prevention. The establishment of the reverse genetic manipulation platform has enabled the development of Eimeria parasites, the live anticoccidial vaccine strains, as vaccine vectors. In our previous study, r...
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| Format: | Article |
| Language: | English |
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BMC
2019-07-01
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| Series: | Parasites & Vectors |
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| Online Access: | http://link.springer.com/article/10.1186/s13071-019-3605-6 |
| _version_ | 1819229045098807296 |
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| author | Xinming Tang Chaoyue Wang Lin Liang Dandan Hu Sixin Zhang Chunhui Duan Jingxia Suo Xianyong Liu Xun Suo Shangjin Cui |
| author_facet | Xinming Tang Chaoyue Wang Lin Liang Dandan Hu Sixin Zhang Chunhui Duan Jingxia Suo Xianyong Liu Xun Suo Shangjin Cui |
| author_sort | Xinming Tang |
| collection | DOAJ |
| description | Abstract Background Live anticoccidial vaccines have been a tremendous success for disease prevention. The establishment of the reverse genetic manipulation platform has enabled the development of Eimeria parasites, the live anticoccidial vaccine strains, as vaccine vectors. In our previous study, recombinant E. tenella expressing a single immunodominant antigen of E. maxima (Et-EmIMP1) was able to protect chickens against challenge infection with E. maxima. This promising result encouraged us to further explore strategies to improve the protection efficacy of recombinant Eimeria and develop it as a vaccine vector. Results We constructed a novel recombinant Eimeria line expressing apical membrane antigen 1 of E. maxima (Et-EmAMA1) and then immunized chickens with Et-EmAMA1 and/or Et-EmIMP1. We found that the E. maxima soluble antigen-specific cell-mediated immunity was much stronger in the birds that were co-immunized with Et-EmAMA1 and Et-EmIMP1 than in those that were immunized with Et-EmAMA1 or Et-EmIMP1 alone. The oocyst production after E. maxima infection was significantly reduced in the recombinant Eimeria-immunized birds compared with the wild-type-immunized and naïve birds. The oocyst production in the birds co-immunized with Et-EmAMA1 and Et-EmIMP1 was consistently the lowest among the treatment groups after E. maxima infection. Conclusions These results demonstrated that Eimeria is an effective vaccine vector that can carry and deliver heterologous Eimeria antigens to the host immune system and trigger specific immune responses. Our results also suggested that increasing the number of recombinant Eimeria lines is an effective approach to enhance protective immunity against infections with heterologous pathogens. |
| first_indexed | 2024-12-23T11:06:55Z |
| format | Article |
| id | doaj.art-3896e36d594542869b8321614596c5b4 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-23T11:06:55Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-3896e36d594542869b8321614596c5b42022-12-21T17:49:28ZengBMCParasites & Vectors1756-33052019-07-011211810.1186/s13071-019-3605-6Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infectionXinming Tang0Chaoyue Wang1Lin Liang2Dandan Hu3Sixin Zhang4Chunhui Duan5Jingxia Suo6Xianyong Liu7Xun Suo8Shangjin Cui9Institute of Animal Science, Chinese Academy of Agricultural SciencesKey Laboratory of Zoonosis of Ministry of Agriculture & National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural UniversityInstitute of Animal Science, Chinese Academy of Agricultural SciencesKey Laboratory of Zoonosis of Ministry of Agriculture & National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural UniversityKey Laboratory of Zoonosis of Ministry of Agriculture & National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural UniversityKey Laboratory of Zoonosis of Ministry of Agriculture & National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural UniversityKey Laboratory of Zoonosis of Ministry of Agriculture & National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural UniversityKey Laboratory of Zoonosis of Ministry of Agriculture & National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural UniversityKey Laboratory of Zoonosis of Ministry of Agriculture & National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural UniversityInstitute of Animal Science, Chinese Academy of Agricultural SciencesAbstract Background Live anticoccidial vaccines have been a tremendous success for disease prevention. The establishment of the reverse genetic manipulation platform has enabled the development of Eimeria parasites, the live anticoccidial vaccine strains, as vaccine vectors. In our previous study, recombinant E. tenella expressing a single immunodominant antigen of E. maxima (Et-EmIMP1) was able to protect chickens against challenge infection with E. maxima. This promising result encouraged us to further explore strategies to improve the protection efficacy of recombinant Eimeria and develop it as a vaccine vector. Results We constructed a novel recombinant Eimeria line expressing apical membrane antigen 1 of E. maxima (Et-EmAMA1) and then immunized chickens with Et-EmAMA1 and/or Et-EmIMP1. We found that the E. maxima soluble antigen-specific cell-mediated immunity was much stronger in the birds that were co-immunized with Et-EmAMA1 and Et-EmIMP1 than in those that were immunized with Et-EmAMA1 or Et-EmIMP1 alone. The oocyst production after E. maxima infection was significantly reduced in the recombinant Eimeria-immunized birds compared with the wild-type-immunized and naïve birds. The oocyst production in the birds co-immunized with Et-EmAMA1 and Et-EmIMP1 was consistently the lowest among the treatment groups after E. maxima infection. Conclusions These results demonstrated that Eimeria is an effective vaccine vector that can carry and deliver heterologous Eimeria antigens to the host immune system and trigger specific immune responses. Our results also suggested that increasing the number of recombinant Eimeria lines is an effective approach to enhance protective immunity against infections with heterologous pathogens.http://link.springer.com/article/10.1186/s13071-019-3605-6Recombinant EimeriaVaccine vectorApical membrane antigen 1Immune mapped protein 1Immune responses |
| spellingShingle | Xinming Tang Chaoyue Wang Lin Liang Dandan Hu Sixin Zhang Chunhui Duan Jingxia Suo Xianyong Liu Xun Suo Shangjin Cui Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection Parasites & Vectors Recombinant Eimeria Vaccine vector Apical membrane antigen 1 Immune mapped protein 1 Immune responses |
| title | Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection |
| title_full | Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection |
| title_fullStr | Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection |
| title_full_unstemmed | Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection |
| title_short | Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection |
| title_sort | co immunization with two recombinant eimeria tenella lines expressing immunoprotective antigens of e maxima elicits enhanced protection against e maxima infection |
| topic | Recombinant Eimeria Vaccine vector Apical membrane antigen 1 Immune mapped protein 1 Immune responses |
| url | http://link.springer.com/article/10.1186/s13071-019-3605-6 |
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