Reciprocal Effect of Copper and Iron Regulation on the Proteome of Synechocystis sp. PCC 6803
Cyanobacteria can acclimate to changing copper and iron concentrations in the environment via metal homeostasis, but a general mechanism for interpreting their dynamic relationships is sparse. In this study, we assessed growth and chlorophyll fluorescence of Synechocystis sp. PCC 6803 and investigat...
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Frontiers Media S.A.
2021-05-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.673402/full |
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| author | Zhang-He Zhen Zhang-He Zhen Song Qin Song Qin Qing-Min Ren Qing-Min Ren Yu Wang Yu-Ying Ma Yin-Chu Wang Yin-Chu Wang |
| author_facet | Zhang-He Zhen Zhang-He Zhen Song Qin Song Qin Qing-Min Ren Qing-Min Ren Yu Wang Yu-Ying Ma Yin-Chu Wang Yin-Chu Wang |
| author_sort | Zhang-He Zhen |
| collection | DOAJ |
| description | Cyanobacteria can acclimate to changing copper and iron concentrations in the environment via metal homeostasis, but a general mechanism for interpreting their dynamic relationships is sparse. In this study, we assessed growth and chlorophyll fluorescence of Synechocystis sp. PCC 6803 and investigated proteomic responses to copper and iron deductions. Results showed that copper and iron exerted reciprocal effect on the growth and photosynthesis of Synechocystis sp. PCC 6803 at combinations of different concentrations. And some proteins involved in the uptake of copper and iron and the photosynthetic electron transport system exhibit Cu–Fe proteomic association. The protein abundance under copper and iron deduction affected the photosynthetic electronic activity of Synechocystis sp. PCC 6803 and eventually affected the growth and photosynthesis. Based on these results, we hypothesize that the Cu–Fe proteomic association of Synechocystis sp. PCC 6803 can be elucidated via the uptake system of outer membrane-periplasmic space-inner plasma membrane-thylakoid membrane, and this association is mainly required to maintain electron transfer. This study provides a broader view regarding the proteomic association between Cu and Fe in cyanobacteria, which will shed light on the role of these two metal elements in cyanobacterial energy metabolism and biomass accumulation. |
| first_indexed | 2024-12-17T08:31:59Z |
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| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-17T08:31:59Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-3899097147424ceb9d93d082c05789e52022-12-21T21:56:34ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-05-01910.3389/fbioe.2021.673402673402Reciprocal Effect of Copper and Iron Regulation on the Proteome of Synechocystis sp. PCC 6803Zhang-He Zhen0Zhang-He Zhen1Song Qin2Song Qin3Qing-Min Ren4Qing-Min Ren5Yu Wang6Yu-Ying Ma7Yin-Chu Wang8Yin-Chu Wang9Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaYantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaYantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaYantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaYantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaYantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaCyanobacteria can acclimate to changing copper and iron concentrations in the environment via metal homeostasis, but a general mechanism for interpreting their dynamic relationships is sparse. In this study, we assessed growth and chlorophyll fluorescence of Synechocystis sp. PCC 6803 and investigated proteomic responses to copper and iron deductions. Results showed that copper and iron exerted reciprocal effect on the growth and photosynthesis of Synechocystis sp. PCC 6803 at combinations of different concentrations. And some proteins involved in the uptake of copper and iron and the photosynthetic electron transport system exhibit Cu–Fe proteomic association. The protein abundance under copper and iron deduction affected the photosynthetic electronic activity of Synechocystis sp. PCC 6803 and eventually affected the growth and photosynthesis. Based on these results, we hypothesize that the Cu–Fe proteomic association of Synechocystis sp. PCC 6803 can be elucidated via the uptake system of outer membrane-periplasmic space-inner plasma membrane-thylakoid membrane, and this association is mainly required to maintain electron transfer. This study provides a broader view regarding the proteomic association between Cu and Fe in cyanobacteria, which will shed light on the role of these two metal elements in cyanobacterial energy metabolism and biomass accumulation.https://www.frontiersin.org/articles/10.3389/fbioe.2021.673402/fullcyanobacteriacopperironproteomededuction |
| spellingShingle | Zhang-He Zhen Zhang-He Zhen Song Qin Song Qin Qing-Min Ren Qing-Min Ren Yu Wang Yu-Ying Ma Yin-Chu Wang Yin-Chu Wang Reciprocal Effect of Copper and Iron Regulation on the Proteome of Synechocystis sp. PCC 6803 Frontiers in Bioengineering and Biotechnology cyanobacteria copper iron proteome deduction |
| title | Reciprocal Effect of Copper and Iron Regulation on the Proteome of Synechocystis sp. PCC 6803 |
| title_full | Reciprocal Effect of Copper and Iron Regulation on the Proteome of Synechocystis sp. PCC 6803 |
| title_fullStr | Reciprocal Effect of Copper and Iron Regulation on the Proteome of Synechocystis sp. PCC 6803 |
| title_full_unstemmed | Reciprocal Effect of Copper and Iron Regulation on the Proteome of Synechocystis sp. PCC 6803 |
| title_short | Reciprocal Effect of Copper and Iron Regulation on the Proteome of Synechocystis sp. PCC 6803 |
| title_sort | reciprocal effect of copper and iron regulation on the proteome of synechocystis sp pcc 6803 |
| topic | cyanobacteria copper iron proteome deduction |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.673402/full |
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