Different factors are operative in shaping the epiphytic grapevine microbiome across different geographical scales: Biogeography, cultivar or vintage?
Abstract Introduction The composition of the grapevine microbiome is controlled by a range of factors all contributing to the establishment of microbial terroir. Most studies have focused on the grape must microbiome, while less is known about the assemblage mechanisms of the epiphytic grapevine mic...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-12-01
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| Series: | Journal of Sustainable Agriculture and Environment |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/sae2.12030 |
| _version_ | 1811207406727725056 |
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| author | Elena Papadopoulou Fotios Bekris Sotirios Vasileiadis Kalliope K. Papadopoulou Dimitrios G. Karpouzas |
| author_facet | Elena Papadopoulou Fotios Bekris Sotirios Vasileiadis Kalliope K. Papadopoulou Dimitrios G. Karpouzas |
| author_sort | Elena Papadopoulou |
| collection | DOAJ |
| description | Abstract Introduction The composition of the grapevine microbiome is controlled by a range of factors all contributing to the establishment of microbial terroir. Most studies have focused on the grape must microbiome, while less is known about the assemblage mechanisms of the epiphytic grapevine microbiome. We aimed to disentangle the role of geographic location, cultivar and vintage on the composition of the epiphytic fungal and bacterial communities of grapevine: within (regional scale) and across (national scale) viticultural zones in Greece. We hypothesised that: (i) the influence of all three factors varies at different geographical scales; (ii) fungi and bacteria respond differently to the factors shaping the grapevine microbiome. Materials and Methods We analysed leaves and berries collected from Greek cultivars (i) Vidiano and Agiorgitiko located in three geographically distant regions (from 100 to >600 km), (ii) Roditis and Sideritis from different terroir units of the viticultural zone of Aigialeia. Results Amplicon sequencing analysis identified strong regional signatures on the epiphytic microbiome across viticultural zones, whereas cultivar becomes a strong determinant at the viticultural zone scale. Fungal communities were more responsive to all studied factors compared to bacterial communities. Differential abundance (DA) analysis identified dominant fungal (Alternaria, Cladosporium, Nigrospora, Aureobasidium, Vishniacozyma) and bacterial (Sphingomonas, Masillia, Streptococcus, Staphylococcus, Enterobacterales) amplicon sequence variants (ASVs) exhibiting geographical and cultivar‐specific patterns. Conclusion Our data suggest that different factors are operative in shaping the epiphytic grapevine microbiome at different geographical scales with bacterial and fungal communities showing different responses to the tested structural factors. |
| first_indexed | 2024-04-12T04:02:29Z |
| format | Article |
| id | doaj.art-389d39a7e4ba4c1c8e2ed21166f93617 |
| institution | Directory Open Access Journal |
| issn | 2767-035X |
| language | English |
| last_indexed | 2024-04-12T04:02:29Z |
| publishDate | 2022-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Sustainable Agriculture and Environment |
| spelling | doaj.art-389d39a7e4ba4c1c8e2ed21166f936172022-12-22T03:48:40ZengWileyJournal of Sustainable Agriculture and Environment2767-035X2022-12-011428730110.1002/sae2.12030Different factors are operative in shaping the epiphytic grapevine microbiome across different geographical scales: Biogeography, cultivar or vintage?Elena Papadopoulou0Fotios Bekris1Sotirios Vasileiadis2Kalliope K. Papadopoulou3Dimitrios G. Karpouzas4Department of Biochemistry and Biotechnology, Laboratory of Plant and Environmental Biotechnology University of Thessaly Viopolis Larissa GreeceDepartment of Biochemistry and Biotechnology, Laboratory of Plant and Environmental Biotechnology University of Thessaly Viopolis Larissa GreeceDepartment of Biochemistry and Biotechnology, Laboratory of Plant and Environmental Biotechnology University of Thessaly Viopolis Larissa GreeceDepartment of Biochemistry and Biotechnology, Laboratory of Plant and Environmental Biotechnology University of Thessaly Viopolis Larissa GreeceDepartment of Biochemistry and Biotechnology, Laboratory of Plant and Environmental Biotechnology University of Thessaly Viopolis Larissa GreeceAbstract Introduction The composition of the grapevine microbiome is controlled by a range of factors all contributing to the establishment of microbial terroir. Most studies have focused on the grape must microbiome, while less is known about the assemblage mechanisms of the epiphytic grapevine microbiome. We aimed to disentangle the role of geographic location, cultivar and vintage on the composition of the epiphytic fungal and bacterial communities of grapevine: within (regional scale) and across (national scale) viticultural zones in Greece. We hypothesised that: (i) the influence of all three factors varies at different geographical scales; (ii) fungi and bacteria respond differently to the factors shaping the grapevine microbiome. Materials and Methods We analysed leaves and berries collected from Greek cultivars (i) Vidiano and Agiorgitiko located in three geographically distant regions (from 100 to >600 km), (ii) Roditis and Sideritis from different terroir units of the viticultural zone of Aigialeia. Results Amplicon sequencing analysis identified strong regional signatures on the epiphytic microbiome across viticultural zones, whereas cultivar becomes a strong determinant at the viticultural zone scale. Fungal communities were more responsive to all studied factors compared to bacterial communities. Differential abundance (DA) analysis identified dominant fungal (Alternaria, Cladosporium, Nigrospora, Aureobasidium, Vishniacozyma) and bacterial (Sphingomonas, Masillia, Streptococcus, Staphylococcus, Enterobacterales) amplicon sequence variants (ASVs) exhibiting geographical and cultivar‐specific patterns. Conclusion Our data suggest that different factors are operative in shaping the epiphytic grapevine microbiome at different geographical scales with bacterial and fungal communities showing different responses to the tested structural factors.https://doi.org/10.1002/sae2.12030biogeographycultivargrapevine microbiometerroirvintage |
| spellingShingle | Elena Papadopoulou Fotios Bekris Sotirios Vasileiadis Kalliope K. Papadopoulou Dimitrios G. Karpouzas Different factors are operative in shaping the epiphytic grapevine microbiome across different geographical scales: Biogeography, cultivar or vintage? Journal of Sustainable Agriculture and Environment biogeography cultivar grapevine microbiome terroir vintage |
| title | Different factors are operative in shaping the epiphytic grapevine microbiome across different geographical scales: Biogeography, cultivar or vintage? |
| title_full | Different factors are operative in shaping the epiphytic grapevine microbiome across different geographical scales: Biogeography, cultivar or vintage? |
| title_fullStr | Different factors are operative in shaping the epiphytic grapevine microbiome across different geographical scales: Biogeography, cultivar or vintage? |
| title_full_unstemmed | Different factors are operative in shaping the epiphytic grapevine microbiome across different geographical scales: Biogeography, cultivar or vintage? |
| title_short | Different factors are operative in shaping the epiphytic grapevine microbiome across different geographical scales: Biogeography, cultivar or vintage? |
| title_sort | different factors are operative in shaping the epiphytic grapevine microbiome across different geographical scales biogeography cultivar or vintage |
| topic | biogeography cultivar grapevine microbiome terroir vintage |
| url | https://doi.org/10.1002/sae2.12030 |
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