Production and verification of novel Osmanthus hybrids
Osmanthus fragrans Lour. is prized for its fragrant, cool-season blooms, smooth leaves, and compact tree form; however, the species is limited in production and use to USDA Hardiness Zones 7 and higher. Increasing the total amount of genetic variability available for evaluation, selection, and breed...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Horticulture |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fhort.2024.1382450/full |
| _version_ | 1797214270104010752 |
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| author | Lisa Alexander Xingbo Wu Fred Gouker Fred Gouker |
| author_facet | Lisa Alexander Xingbo Wu Fred Gouker Fred Gouker |
| author_sort | Lisa Alexander |
| collection | DOAJ |
| description | Osmanthus fragrans Lour. is prized for its fragrant, cool-season blooms, smooth leaves, and compact tree form; however, the species is limited in production and use to USDA Hardiness Zones 7 and higher. Increasing the total amount of genetic variability available for evaluation, selection, and breeding is foundational to the development of novel Osmanthus cultivars with increased cold-hardiness and improved ornamental characteristics such as high rooting rates, faster growth, fragrant flowers, and an evergreen or semi-evergreen habit. Hybridization among Osmanthus species should generate novel phenotypes and allow trait introgression among populations and breeding lines. The objectives of this study were to produce, verify, and evaluate Osmanthus hybrids by making controlled and open-pollinated crosses among Osmanthus species, using SSR markers to determine parentage of cross progeny, and comparing flowering, leaf shape, and form of hybrids offspring with parents. Fifty-eight of 66 progenies (88%) were assigned paternity at the 95% confidence level or above and 47 progenies (71%) were assigned paternity at the 99% confidence level. Novel Osmanthus hybrids were produced including O. armatus × O. fortunei, O. armatus × O. heterophyllus, and O. armatus × O. fragrans. Open-pollinated families increased in height an average of 204% and 159% per year in the first and second growing seasons, respectively. Male flower type was significantly associated with progeny flower type (χ2 = 17.5, p < 0.0001) while leaf margin was not associated with cross family (χ2 = 0.83, p = 0.84). Evaluation of more Osmanthus accessions and subsequent hybridizations will be necessary to introgress favorable genes and generate the variation necessary to expand the genus into new markets. |
| first_indexed | 2024-04-24T11:11:30Z |
| format | Article |
| id | doaj.art-389ebb62c4394a44bed17a6a1c9848f6 |
| institution | Directory Open Access Journal |
| issn | 2813-3595 |
| language | English |
| last_indexed | 2024-04-24T11:11:30Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Horticulture |
| spelling | doaj.art-389ebb62c4394a44bed17a6a1c9848f62024-04-11T13:09:09ZengFrontiers Media S.A.Frontiers in Horticulture2813-35952024-04-01310.3389/fhort.2024.13824501382450Production and verification of novel Osmanthus hybridsLisa Alexander0Xingbo Wu1Fred Gouker2Fred Gouker3Floral and Nursery Plants Research Unit, Agricultural Research Service, U.S. Department of Agriculture, McMinnville, TN, United StatesTropical Research and Education Center, Department of Environmental Horticulture, University of Florida, Homestead, FL, United StatesFloral and Nursery Plants Research Unit, Agricultural Research Service, U.S. Department of Agriculture, McMinnville, TN, United StatesGrapes Genetics Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Geneva, NY, United StatesOsmanthus fragrans Lour. is prized for its fragrant, cool-season blooms, smooth leaves, and compact tree form; however, the species is limited in production and use to USDA Hardiness Zones 7 and higher. Increasing the total amount of genetic variability available for evaluation, selection, and breeding is foundational to the development of novel Osmanthus cultivars with increased cold-hardiness and improved ornamental characteristics such as high rooting rates, faster growth, fragrant flowers, and an evergreen or semi-evergreen habit. Hybridization among Osmanthus species should generate novel phenotypes and allow trait introgression among populations and breeding lines. The objectives of this study were to produce, verify, and evaluate Osmanthus hybrids by making controlled and open-pollinated crosses among Osmanthus species, using SSR markers to determine parentage of cross progeny, and comparing flowering, leaf shape, and form of hybrids offspring with parents. Fifty-eight of 66 progenies (88%) were assigned paternity at the 95% confidence level or above and 47 progenies (71%) were assigned paternity at the 99% confidence level. Novel Osmanthus hybrids were produced including O. armatus × O. fortunei, O. armatus × O. heterophyllus, and O. armatus × O. fragrans. Open-pollinated families increased in height an average of 204% and 159% per year in the first and second growing seasons, respectively. Male flower type was significantly associated with progeny flower type (χ2 = 17.5, p < 0.0001) while leaf margin was not associated with cross family (χ2 = 0.83, p = 0.84). Evaluation of more Osmanthus accessions and subsequent hybridizations will be necessary to introgress favorable genes and generate the variation necessary to expand the genus into new markets.https://www.frontiersin.org/articles/10.3389/fhort.2024.1382450/fulltea olivehybrid breedingmicrosatelliteSSRpaternity analysisgermplasm |
| spellingShingle | Lisa Alexander Xingbo Wu Fred Gouker Fred Gouker Production and verification of novel Osmanthus hybrids Frontiers in Horticulture tea olive hybrid breeding microsatellite SSR paternity analysis germplasm |
| title | Production and verification of novel Osmanthus hybrids |
| title_full | Production and verification of novel Osmanthus hybrids |
| title_fullStr | Production and verification of novel Osmanthus hybrids |
| title_full_unstemmed | Production and verification of novel Osmanthus hybrids |
| title_short | Production and verification of novel Osmanthus hybrids |
| title_sort | production and verification of novel osmanthus hybrids |
| topic | tea olive hybrid breeding microsatellite SSR paternity analysis germplasm |
| url | https://www.frontiersin.org/articles/10.3389/fhort.2024.1382450/full |
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