Synthesis and Characterization of Ciprofloxacin-loaded Chitosan Nanoparticles for Eradication of Pseudomonas aeroginosa Biofilm

Background: Pseudomonas aeruginosa biofilm is one of the problems in antibiotic treatment of infections. Nanomedicines, such as chitosan (CS) can carry multiple drugs and improve the therapeutic effects of antibiotics. Objectives: This study aimed at the synthesis and characterization of ciprofloxac...

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Main Authors: Foroogh Faridi, Nima Bahador, Saeed Shoja, Sahar Abbasi
Format: Article
Language:English
Published: Mazandaran University of Medical Sciences 2023-07-01
Series:Pharmaceutical and Biomedical Research
Subjects:
Online Access:http://pbr.mazums.ac.ir/article-1-494-en.pdf
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author Foroogh Faridi
Nima Bahador
Saeed Shoja
Sahar Abbasi
author_facet Foroogh Faridi
Nima Bahador
Saeed Shoja
Sahar Abbasi
author_sort Foroogh Faridi
collection DOAJ
description Background: Pseudomonas aeruginosa biofilm is one of the problems in antibiotic treatment of infections. Nanomedicines, such as chitosan (CS) can carry multiple drugs and improve the therapeutic effects of antibiotics. Objectives: This study aimed at the synthesis and characterization of ciprofloxacin-loaded chitosan nanoparticles for eradication of P. aeroginosa biofilm. Methods: Cipro-CS microparticles were prepared by ionic gelation method and their size, zeta potential, and drug release pattern were determined. MBEC and MBIC of different groups of antibiotics (ciprofloxacin, ciprofloxacin-PAβN, CS ciprofloxacin, and CS ciprofloxacin-PAβN) were performed on biofilm samples of P. aeroginosa.  Results: Ciprofloxacin loading efficiency was 35.51%, and encapsulation efficiency was 55.06%. Released ciprofloxacin from CS nanoparticles was 80% after 24 hours. Biofilm production was positive in 96.7% of the isolates while 30.1% of the samples had strong biofilm. The best result for MBIC was CS ciprofloxacin, CS ciprofloxacin-PAβN, ciprofloxacin- PAβN, and ciprofloxacin, respectively. For MBEC the result was slightly different and from the best to better CS Ciprofloxacin-PAβN, CS Ciprofloxacin, Ciprofloxacin-PAβN, and ciprofloxacin. Conclusion: Today, with increasing antibiotic resistance, there are many challenges in treating infections. Due to the role of biofilm in antibiotic resistance, researchers are looking for new antibiotics to treat infections.
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spelling doaj.art-38a263c7a8d3439da51c83d70758566d2023-09-14T07:45:55ZengMazandaran University of Medical SciencesPharmaceutical and Biomedical Research2423-44942023-07-0193243258Synthesis and Characterization of Ciprofloxacin-loaded Chitosan Nanoparticles for Eradication of Pseudomonas aeroginosa BiofilmForoogh Faridi0Nima Bahador1Saeed Shoja2Sahar Abbasi3 Department of Microbiology, Faculty of Science, Agriculture and New Technologies, Shiraz Branch, Islamic Azad University, Shiraz, Iran. Department of Microbiology, Faculty of Science, Agriculture and New Technologies, Shiraz Branch, Islamic Azad University, Shiraz, Iran. Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran. Department of Pharmaceutics, Faculty of Pharmacy, Hormozgan University of Medical Sciences, Bandar Abbas, Iran. Background: Pseudomonas aeruginosa biofilm is one of the problems in antibiotic treatment of infections. Nanomedicines, such as chitosan (CS) can carry multiple drugs and improve the therapeutic effects of antibiotics. Objectives: This study aimed at the synthesis and characterization of ciprofloxacin-loaded chitosan nanoparticles for eradication of P. aeroginosa biofilm. Methods: Cipro-CS microparticles were prepared by ionic gelation method and their size, zeta potential, and drug release pattern were determined. MBEC and MBIC of different groups of antibiotics (ciprofloxacin, ciprofloxacin-PAβN, CS ciprofloxacin, and CS ciprofloxacin-PAβN) were performed on biofilm samples of P. aeroginosa.  Results: Ciprofloxacin loading efficiency was 35.51%, and encapsulation efficiency was 55.06%. Released ciprofloxacin from CS nanoparticles was 80% after 24 hours. Biofilm production was positive in 96.7% of the isolates while 30.1% of the samples had strong biofilm. The best result for MBIC was CS ciprofloxacin, CS ciprofloxacin-PAβN, ciprofloxacin- PAβN, and ciprofloxacin, respectively. For MBEC the result was slightly different and from the best to better CS Ciprofloxacin-PAβN, CS Ciprofloxacin, Ciprofloxacin-PAβN, and ciprofloxacin. Conclusion: Today, with increasing antibiotic resistance, there are many challenges in treating infections. Due to the role of biofilm in antibiotic resistance, researchers are looking for new antibiotics to treat infections.http://pbr.mazums.ac.ir/article-1-494-en.pdfchitosannanoparticlebiofilmpseudomonas aeruginosa
spellingShingle Foroogh Faridi
Nima Bahador
Saeed Shoja
Sahar Abbasi
Synthesis and Characterization of Ciprofloxacin-loaded Chitosan Nanoparticles for Eradication of Pseudomonas aeroginosa Biofilm
Pharmaceutical and Biomedical Research
chitosan
nanoparticle
biofilm
pseudomonas aeruginosa
title Synthesis and Characterization of Ciprofloxacin-loaded Chitosan Nanoparticles for Eradication of Pseudomonas aeroginosa Biofilm
title_full Synthesis and Characterization of Ciprofloxacin-loaded Chitosan Nanoparticles for Eradication of Pseudomonas aeroginosa Biofilm
title_fullStr Synthesis and Characterization of Ciprofloxacin-loaded Chitosan Nanoparticles for Eradication of Pseudomonas aeroginosa Biofilm
title_full_unstemmed Synthesis and Characterization of Ciprofloxacin-loaded Chitosan Nanoparticles for Eradication of Pseudomonas aeroginosa Biofilm
title_short Synthesis and Characterization of Ciprofloxacin-loaded Chitosan Nanoparticles for Eradication of Pseudomonas aeroginosa Biofilm
title_sort synthesis and characterization of ciprofloxacin loaded chitosan nanoparticles for eradication of pseudomonas aeroginosa biofilm
topic chitosan
nanoparticle
biofilm
pseudomonas aeruginosa
url http://pbr.mazums.ac.ir/article-1-494-en.pdf
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